Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(Sp7/mCherry)2Pmay
Name:	transgene insertion 2, Peter Maye
MGI ID:	MGI:5556386
Synonyms:	Osterix-Cherry, Osterix-mCherry
Transgene:	Tg(Sp7/mCherry)2Pmay  Location: unknown
Alliance:	Tg(Sp7/mCherry)2Pmay page

Strain of Origin:

CD-1

Transgene Type:    Transgenic (Reporter) Mutation:    Insertion   Mutation details: Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a 39 kb BAC (#RP24-362M3) containing the entire mouse Sp7 gene. This BAC was modified to fuse the first 17 amino acids of Sp7 to the N-terminal of the red fluorescent protein (Cherry) sequence upstream of the second translation start site. This transgene contains two translational start sites; the first is located in exon 1b and results in a long protein isoform containing 428 amino acids. The second start site is in-frame with upstream start site located at the beginning of exon 2 and results in a short protein isoform containing 410 amino acids. The Cherry reporter can be used to detect both isoforms. Strong reporter expression is observed in mature osteoblasts and osteocytes; weak expression is observed in bone marrow. (J:195100)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:195100 Strecker S, et al., Generation and characterization of Osterix-Cherry reporter mice. Genesis. 2013 Apr;51(4):246-58
All:	11 reference(s)