Probe:	Sost probe2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: heart	Absent		1A/E

Structure	Level	Pattern	Image	Note
TS19: heart	Absent		1B/F

Structure	Level	Pattern	Image	Note
TS21: heart	Absent		1C/G

Structure	Level	Pattern	Image	Note
TS22: heart	Absent		1D/H

Structure	Level	Pattern	Image	Note
TS23: aorta	Present		1I/J/K
TS23: pulmonary trunk	Present		1I/J/K
TS23: heart	Absent		1I/J/K	No expression in the heart, including the valves and coronary arteries.
TS23: heart valve	Absent		1I/J/K
TS23: pulmonary artery	Absent		1I/J/K	No expression in the branching right and left pulmonary arteries.
TS23: common carotid artery	Present		1I/J/K	Both the right and left common carotids.
TS23: innominate artery	Present		1I/J/K	Brachiocephalic artery.
TS23: esophagus mesenchyme	Absent		1I/J/K	No expression in the visceral smooth muscle cells.
TS23: trachea mesenchyme	Absent		1I/J/K	No expression in the visceral smooth muscle cells.
TS23: interventricular septum	Absent		1I/J/K	No expression in the neural crest-derived outflow septum between the aortic and pulmonary valves.

Structure	Level	Pattern	Image	Note
TS23: arch of aorta	Present		1L/M
TS23: ascending aorta	Present		1L/M
TS23: descending thoracic aorta	Absent		1L/M

Structure	Level	Pattern	Image	Note
TS23: ductus arteriosus	Present		1N/O	Expressed in a lesser extent.
TS23: pulmonary artery	Absent		1N/O

Structure	Level	Pattern	Image	Note
TS25: common carotid artery	Present		1P/Q	Both the right and left common carotids.
TS25: innominate artery	Present		1P/Q	Brachiocephalic artery.
TS25: subclavian artery	Absent		1P/Q	The right and left subclavian artery were both negative.
TS25: vertebra	Present		1P/Q

Structure	Level	Pattern	Image	Note
TS23: pulmonary trunk	Present	Regionally restricted	1R/S	Expressed in the vessel wall with smooth muscle cells, while the first cell layer of endothelial cells is negative.

Structure	Level	Pattern	Image	Note
TS27: arch of aorta	Present	Regionally restricted	2A/B/C	Expressed in the medial vessel wall.
TS27: ascending aorta	Present	Regionally restricted	2A/B/C	Expressed in the medial vessel wall.
TS27: common carotid artery	Present	Regionally restricted	2A/B/C	Expressed in the medial vessel wall.
TS27: atrioventricular valve	Absent		2A/B/C
TS27: atrium cardiac muscle	Absent		2A/B/C
TS27: descending aorta	Absent		2A/B/C
TS27: endocardial tissue	Absent		2A/B/C
TS27: heart	Absent		2A/B/C
TS27: pulmonary artery	Absent		2A/B/C
TS27: semilunar valve	Absent		2A/B/C
TS27: ventricle cardiac muscle	Absent		2A/B/C

Structure	Level	Pattern	Image	Note
TS27: arch of aorta	Present	Regionally restricted	2D/E	Expressed in the medial vessel wall.
TS27: pulmonary trunk	Present	Regionally restricted	2D/E	Expressed in the medial vessel wall.
TS27: ductus arteriosus	Absent		2D/E

Structure	Level	Pattern	Image	Note
TS28: aortic valve	Absent		2F/G
TS28: ascending aorta	Absent		2F/G