Gene Expression Data
Assay Details
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Assay
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Reference:
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J:163739
Leung AW, et al., Loss of procollagen IIA from the anterior mesendoderm disrupts the development of mouse embryonic forebrain. Dev Dyn. 2010 Sep;239(9):2319-29
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5554578
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Gene symbol:
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Six3
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Gene name:
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sine oculis-related homeobox 3
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Probe:
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Six3 probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5E:
embryonic day 7.5
(more  )
Note: Neural plate stage.
Specimen
5E:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Neural plate stage.
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Specimen
5F:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Col2a1tm1.1Ksec/Col2a1tm1.1Ksec
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Neural plate stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: neural ectoderm |
Present |
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5F
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Normal expression was detected in the neural precursor tissues in the mutant.
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Specimen
5G:
embryonic day 8.0
(more )
Note: Early headfold stage.
Specimen
5G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Early headfold stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: head |
Present |
Regionally restricted |
5G
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Expression was detected in the head folds.
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Specimen
5H:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Col2a1tm1.1Ksec/Col2a1tm1.1Ksec
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Early headfold stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: head |
Present |
Regionally restricted |
5H
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The expression domain in the head folds was reduced in the mutant compared to wild type.
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Specimen
5I:
embryonic day 8.25
(more )
Note: Early somite stage.
Specimen
5I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Early somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: head |
Present |
Regionally restricted |
5I
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Expression was detected in the head folds.
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Specimen
5J:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Col2a1tm1.1Ksec/Col2a1tm1.1Ksec
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Age:
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embryonic day 8.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Early somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: head |
Present |
Regionally restricted |
5J
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The expression domain in the head folds was reduced in the mutant compared to wild type.
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Analysis Tools