Smad9 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:163739 Leung AW, et al., Loss of procollagen IIA from the anterior mesendoderm disrupts the development of mouse embryonic forebrain. Dev Dyn. 2010 Sep;239(9):2319-29
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5554577
Gene symbol:	Smad9
Gene name:	SMAD family member 9

Antibody:	Anti-Phospho-Smad1/5/8
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	The antibody recognizes the phosphorylated form of the protein.

Results

Specimen S4 +/- whole mount: embryonic day 8.0; Col2a1^tm1.1Ksec/Col2a1⁺ (more

)
Note: 3 somites.

Structure	Level	Pattern	Image	Note
TS12: yolk sac	Present		S4_+/- whole mount
TS12: foregut diverticulum	Present	Regionally restricted	S4_+/- whole mount	Expression was detected in foregut epithelium.

Specimen S4 -/- whole mount: embryonic day 8.0; Col2a1^tm1.1Ksec/Col2a1^tm1.1Ksec (more

)
Note: 3 somites.

Structure	Level	Pattern	Image	Note
TS12: yolk sac	Present		S4_-/- whole mount	No change in the population of positive cells was noted in the mutant compared to wild type.
TS12: foregut diverticulum	Present	Regionally restricted	S4_-/- whole mount	Expression was detected in foregut epithelium. No change in the population of positive cells was noted in the mutant compared to wild type.

Specimen S4 +/- section: embryonic day 8.0; Col2a1^tm1.1Ksec/Col2a1⁺ (more

)
Note: 3 somites.

Structure	Level	Pattern	Image	Note
TS12: yolk sac	Present		S4_+/- section
TS12: foregut diverticulum	Present	Regionally restricted	S4_+/- section	Expression was detected in foregut epithelium.

Specimen S4 -/- section: embryonic day 8.0; Col2a1^tm1.1Ksec/Col2a1^tm1.1Ksec (more

)
Note: 3 somites.

Structure	Level	Pattern	Image	Note
TS12: yolk sac	Present		S4_-/- section	No change in the population of positive cells was noted in the mutant compared to wild type.
TS12: foregut diverticulum	Present	Regionally restricted	S4_-/- section	Expression was detected in foregut epithelium. No change in the population of positive cells was noted in the mutant compared to wild type.

Specimen not shown late headfold: embryonic day 8.0 (more

)
Note: Late headfold stage. Table 2.

Structure	Level	Pattern	Note
TS11: mesendoderm	Present	Regionally restricted	Expression was detected in anterior mesendoderm.
TS11: head mesenchyme	Present
TS11: neural ectoderm	Present

Specimen not shown late headfold -/-: embryonic day 8.0; Col2a1^tm1.1Ksec/Col2a1^tm1.1Ksec (more

)
Note: Late headfold stage. Table 2.

Structure	Level	Pattern	Note
TS11: mesendoderm	Present	Regionally restricted	Expression was detected in anterior mesendoderm. No change in the population of positive cells was noted in the mutant compared to wild type.
TS11: head mesenchyme	Present		No change in the population of positive cells was noted in the mutant compared to wild type.
TS11: neural ectoderm	Present		No change in the population of positive cells was noted in the mutant compared to wild type.

Specimen not shown 4s: embryonic day 8.0 (more

)
Note: 4 somites. Table 2.

Structure	Level	Pattern	Note
TS12: embryo	Present	Regionally restricted	Expression was detected in anterior mesendoderm.
TS12: future prosencephalon floor plate	Present
TS12: head mesenchyme	Present
TS12: neural ectoderm	Present

Specimen not shown 4s -/-: embryonic day 8.0; Col2a1^tm1.1Ksec/Col2a1^tm1.1Ksec (more

)
Note: 4 somites. Table 2.

Structure	Level	Pattern	Note
TS12: embryo	Present	Regionally restricted	Expression was detected in anterior mesendoderm. No change in the population of positive cells was noted in the mutant compared to wild type.
TS12: future prosencephalon floor plate	Present		No change in the population of positive cells was noted in the mutant compared to wild type.
TS12: head mesenchyme	Present		No change in the population of positive cells was noted in the mutant compared to wild type.
TS12: neural ectoderm	Present		No change in the population of positive cells was noted in the mutant compared to wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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