Smad9 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:163739 Leung AW, et al., Loss of procollagen IIA from the anterior mesendoderm disrupts the development of mouse embryonic forebrain. Dev Dyn. 2010 Sep;239(9):2319-29
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5554577
Gene symbol:	Smad9
Gene name:	SMAD family member 9

Antibody:	Anti-Phospho-Smad1/5/8
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	The antibody recognizes the phosphorylated form of the protein.

Results

Specimen S4 +/- whole mount: embryonic day 8.0; Col2a1^tm1.1Ksec/Col2a1⁺ (more

)
Note: 3 somites.

Structure	Level	Pattern	Image	Note
TS12: yolk sac	Present		S4_+/- whole mount
TS12: foregut diverticulum	Present	Regionally restricted	S4_+/- whole mount	Expression was detected in foregut epithelium.

Specimen S4 -/- whole mount: embryonic day 8.0; Col2a1^tm1.1Ksec/Col2a1^tm1.1Ksec (more

)
Note: 3 somites.

Structure	Level	Pattern	Image	Note
TS12: yolk sac	Present		S4_-/- whole mount	No change in the population of positive cells was noted in the mutant compared to wild type.
TS12: foregut diverticulum	Present	Regionally restricted	S4_-/- whole mount	Expression was detected in foregut epithelium. No change in the population of positive cells was noted in the mutant compared to wild type.

Specimen S4 +/- section: embryonic day 8.0; Col2a1^tm1.1Ksec/Col2a1⁺ (more

)
Note: 3 somites.

Structure	Level	Pattern	Image	Note
TS12: yolk sac	Present		S4_+/- section
TS12: foregut diverticulum	Present	Regionally restricted	S4_+/- section	Expression was detected in foregut epithelium.

Specimen S4 -/- section: embryonic day 8.0; Col2a1^tm1.1Ksec/Col2a1^tm1.1Ksec (more

)
Note: 3 somites.

Structure	Level	Pattern	Image	Note
TS12: yolk sac	Present		S4_-/- section	No change in the population of positive cells was noted in the mutant compared to wild type.
TS12: foregut diverticulum	Present	Regionally restricted	S4_-/- section	Expression was detected in foregut epithelium. No change in the population of positive cells was noted in the mutant compared to wild type.

Specimen not shown late headfold: embryonic day 8.0 (more

)
Note: Late headfold stage. Table 2.

Structure	Level	Pattern	Note
TS11: mesendoderm	Present	Regionally restricted	Expression was detected in anterior mesendoderm.
TS11: head mesenchyme	Present
TS11: neural ectoderm	Present

Specimen not shown late headfold -/-: embryonic day 8.0; Col2a1^tm1.1Ksec/Col2a1^tm1.1Ksec (more

)
Note: Late headfold stage. Table 2.

Structure	Level	Pattern	Note
TS11: mesendoderm	Present	Regionally restricted	Expression was detected in anterior mesendoderm. No change in the population of positive cells was noted in the mutant compared to wild type.
TS11: head mesenchyme	Present		No change in the population of positive cells was noted in the mutant compared to wild type.
TS11: neural ectoderm	Present		No change in the population of positive cells was noted in the mutant compared to wild type.

Specimen not shown 4s: embryonic day 8.0 (more

)
Note: 4 somites. Table 2.

Structure	Level	Pattern	Note
TS12: embryo	Present	Regionally restricted	Expression was detected in anterior mesendoderm.
TS12: future prosencephalon floor plate	Present
TS12: head mesenchyme	Present
TS12: neural ectoderm	Present

Specimen not shown 4s -/-: embryonic day 8.0; Col2a1^tm1.1Ksec/Col2a1^tm1.1Ksec (more

)
Note: 4 somites. Table 2.

Structure	Level	Pattern	Note
TS12: embryo	Present	Regionally restricted	Expression was detected in anterior mesendoderm. No change in the population of positive cells was noted in the mutant compared to wild type.
TS12: future prosencephalon floor plate	Present		No change in the population of positive cells was noted in the mutant compared to wild type.
TS12: head mesenchyme	Present		No change in the population of positive cells was noted in the mutant compared to wild type.
TS12: neural ectoderm	Present		No change in the population of positive cells was noted in the mutant compared to wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24