Pitx2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:116645 Cota CD, et al., Mice with mutations in Mahogunin ring finger-1 (Mgrn1) exhibit abnormal patterning of the left-right axis. Dev Dyn. 2006 Dec;235(12):3438-47
Assay type:	RNA in situ
MGI Accession ID:	MGI:5553471
Gene symbol:	Pitx2
Gene name:	paired-like homeodomain transcription factor 2

Probe:	Rieg probe
Probe preparation:	Antisense
Assay notes:	Data was shown in Table 4.

Results

Specimen +/+ 5-7: embryonic day 8.0 (more

)
Note: 5-7 somites.

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted		Asymmetrically expressed in the left lateral plate mesenchyme.

Specimen +/+ 8-12: embryonic day 8.5 (more

)
Note: 8-12 somites.

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Present	Regionally restricted		Asymmetrically expressed in the left lateral plate mesenchyme.

Specimen +/+ 13-21: embryonic day 9.0 (more

)
Note: 13-21 somites.

Structure	Level	Pattern	Image	Note
TS14: mesenchyme derived from lateral plate	Present	Regionally restricted		Asymmetrically expressed in the left lateral plate mesenchyme.

Specimen md-nc/md-nc 5-7 variant 1: embryonic day 8.0; Mgrn1^md-nc/Mgrn1^md-nc (more

)
Note: 5-7 somites.

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted		50% showed asymmetric expression in the left lateral plate mesenchyme. 8.3% showed right-side expression.

Specimen md-nc/md-nc 5-7 variant 2: embryonic day 8.0; Mgrn1^md-nc/Mgrn1^md-nc (more

)
Note: 5-7 somites.

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Absent			41.7% showed absent expression.

Specimen md-nc/md-nc 8-12 variant 1: embryonic day 8.5; Mgrn1^md-nc/Mgrn1^md-nc (more

)
Note: 8-12 somites.

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Present	Regionally restricted		50% showed asymmetric expression in the left lateral plate mesenchyme. 8.3% showed right-side expression.

Specimen md-nc/md-nc 8-12 variant 2: embryonic day 8.5; Mgrn1^md-nc/Mgrn1^md-nc (more

)
Note: 8-12 somites.

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Absent			41.7% showed absent expression.

Specimen md-nc/md-nc 13-21 variant 1: embryonic day 9.0; Mgrn1^md-nc/Mgrn1^md-nc (more

)
Note: 13-21 somites.

Structure	Level	Pattern	Image	Note
TS14: mesenchyme derived from lateral plate	Present	Regionally restricted		66.6% showed asymmetric expression in the left lateral plate mesenchyme. 16.7% showed right-side expression.

Specimen md-nc/md-nc 13-21 variant 2: embryonic day 9.0; Mgrn1^md-nc/Mgrn1^md-nc (more

)
Note: 13-21 somites.

Structure	Level	Pattern	Image	Note
TS14: mesenchyme derived from lateral plate	Absent			16.7% showed absent expression.

Specimen md-2J/md-2J 5-7 variant 1: embryonic day 8.0; Mgrn1^md-2J/Mgrn1^md-2J (more

)
Note: 5-7 somites.

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted		71.5% showed normal left-side expression. 7.1% showed bilateral expression.

Specimen md-2J/md-2J 5-7 variant 2: embryonic day 8.0; Mgrn1^md-2J/Mgrn1^md-2J (more

)
Note: 5-7 somites.

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Absent			21.4% showed absent expression.

Specimen md-2J/md-2J 8-12 variant 1: embryonic day 8.5; Mgrn1^md-2J/Mgrn1^md-2J (more

)
Note: 8-12 somites.

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Present	Regionally restricted		71.5% showed normal left-side expression. 7.1% showed bilateral expression.

Specimen md-2J/md-2J 8-12 variant 2: embryonic day 8.5; Mgrn1^md-2J/Mgrn1^md-2J (more

)
Note: 8-12 somites.

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Absent			21.4% showed absent expression.

Specimen md-2J/md-2J 13-21 variant 1: embryonic day 9.0; Mgrn1^md-2J/Mgrn1^md-2J (more

)
Note: 13-21 somites.

Structure	Level	Pattern	Image	Note
TS14: mesenchyme derived from lateral plate	Present	Regionally restricted		73.7% showed normal left-side expression. 10.5% showed bilateral expression.

Specimen md-2J/md-2J 13-21 variant 2: embryonic day 9.0; Mgrn1^md-2J/Mgrn1^md-2J (more

)
Note: 13-21 somites.

Structure	Level	Pattern	Image	Note
TS14: mesenchyme derived from lateral plate	Absent			15.8% showed absent expression.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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