Pitx2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:116645 Cota CD, et al., Mice with mutations in Mahogunin ring finger-1 (Mgrn1) exhibit abnormal patterning of the left-right axis. Dev Dyn. 2006 Dec;235(12):3438-47
Assay type:	RNA in situ
MGI Accession ID:	MGI:5553471
Gene symbol:	Pitx2
Gene name:	paired-like homeodomain transcription factor 2

Probe:	Rieg probe
Probe preparation:	Antisense
Assay notes:	Data was shown in Table 4.

Results

Specimen +/+ 5-7: embryonic day 8.0 (more

)
Note: 5-7 somites.

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted		Asymmetrically expressed in the left lateral plate mesenchyme.

Specimen +/+ 8-12: embryonic day 8.5 (more

)
Note: 8-12 somites.

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Present	Regionally restricted		Asymmetrically expressed in the left lateral plate mesenchyme.

Specimen +/+ 13-21: embryonic day 9.0 (more

)
Note: 13-21 somites.

Structure	Level	Pattern	Image	Note
TS14: mesenchyme derived from lateral plate	Present	Regionally restricted		Asymmetrically expressed in the left lateral plate mesenchyme.

Specimen md-nc/md-nc 5-7 variant 1: embryonic day 8.0; Mgrn1^md-nc/Mgrn1^md-nc (more

)
Note: 5-7 somites.

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted		50% showed asymmetric expression in the left lateral plate mesenchyme. 8.3% showed right-side expression.

Specimen md-nc/md-nc 5-7 variant 2: embryonic day 8.0; Mgrn1^md-nc/Mgrn1^md-nc (more

)
Note: 5-7 somites.

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Absent			41.7% showed absent expression.

Specimen md-nc/md-nc 8-12 variant 1: embryonic day 8.5; Mgrn1^md-nc/Mgrn1^md-nc (more

)
Note: 8-12 somites.

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Present	Regionally restricted		50% showed asymmetric expression in the left lateral plate mesenchyme. 8.3% showed right-side expression.

Specimen md-nc/md-nc 8-12 variant 2: embryonic day 8.5; Mgrn1^md-nc/Mgrn1^md-nc (more

)
Note: 8-12 somites.

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Absent			41.7% showed absent expression.

Specimen md-nc/md-nc 13-21 variant 1: embryonic day 9.0; Mgrn1^md-nc/Mgrn1^md-nc (more

)
Note: 13-21 somites.

Structure	Level	Pattern	Image	Note
TS14: mesenchyme derived from lateral plate	Present	Regionally restricted		66.6% showed asymmetric expression in the left lateral plate mesenchyme. 16.7% showed right-side expression.

Specimen md-nc/md-nc 13-21 variant 2: embryonic day 9.0; Mgrn1^md-nc/Mgrn1^md-nc (more

)
Note: 13-21 somites.

Structure	Level	Pattern	Image	Note
TS14: mesenchyme derived from lateral plate	Absent			16.7% showed absent expression.

Specimen md-2J/md-2J 5-7 variant 1: embryonic day 8.0; Mgrn1^md-2J/Mgrn1^md-2J (more

)
Note: 5-7 somites.

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted		71.5% showed normal left-side expression. 7.1% showed bilateral expression.

Specimen md-2J/md-2J 5-7 variant 2: embryonic day 8.0; Mgrn1^md-2J/Mgrn1^md-2J (more

)
Note: 5-7 somites.

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Absent			21.4% showed absent expression.

Specimen md-2J/md-2J 8-12 variant 1: embryonic day 8.5; Mgrn1^md-2J/Mgrn1^md-2J (more

)
Note: 8-12 somites.

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Present	Regionally restricted		71.5% showed normal left-side expression. 7.1% showed bilateral expression.

Specimen md-2J/md-2J 8-12 variant 2: embryonic day 8.5; Mgrn1^md-2J/Mgrn1^md-2J (more

)
Note: 8-12 somites.

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Absent			21.4% showed absent expression.

Specimen md-2J/md-2J 13-21 variant 1: embryonic day 9.0; Mgrn1^md-2J/Mgrn1^md-2J (more

)
Note: 13-21 somites.

Structure	Level	Pattern	Image	Note
TS14: mesenchyme derived from lateral plate	Present	Regionally restricted		73.7% showed normal left-side expression. 10.5% showed bilateral expression.

Specimen md-2J/md-2J 13-21 variant 2: embryonic day 9.0; Mgrn1^md-2J/Mgrn1^md-2J (more

)
Note: 13-21 somites.

Structure	Level	Pattern	Image	Note
TS14: mesenchyme derived from lateral plate	Absent			15.8% showed absent expression.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 01/28/2026 MGI 6.24