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Gene Expression Data
Assay Details
Assay
Reference: J:202055 Siyanov V, et al., NHE1 is the sodium-hydrogen exchanger active in acute intracellular pH regulation in preimplantation mouse embryos. Biol Reprod. 2013 Jun;88(6):157
Assay type: RT-PCR
MGI Accession ID: MGI:5553338
Gene symbol: Slc9a3
Gene name: solute carrier family 9 (sodium/hydrogen exchanger), member 3
Probe: Slc9a3-pA, Slc9a3-pB
Visualized with: Ethidium bromide
Assay notes: These reactions were repeated on at least four separate oocyte or embryo collections. Each reaction contained an amount of cDNA template equal to 1.5 oocytes or embryos. Identities of products were confirmed by direct sequencing of sample bands.
Results Image: 1A Slc9a3
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
Lane 1 Mol. Wt. Marker Lane
[+] P TS28: liver Present Not Specified Not Specified Not Specified
[-] Control
GV P (a) TS28: primary oocyte Present Not Specified CF-1 Female germinal vesicle oocytes
E P (a) TS28: secondary oocyte Present Not Specified CF-1 Female metaphase II eggs
1C E0.5 (a) TS1: 1-cell stage conceptus Present Not Specified involves: C57BL/6 * CF-1 * DBA/2 Not Specified one-cell embryos
2C E1.0 (a) TS2: 2-cell stage conceptus Trace Not Specified involves: C57BL/6 * CF-1 * DBA/2 Not Specified two-cell embryos
4C E2.0 (a) TS3: 4-cell stage conceptus Absent Not Specified involves: C57BL/6 * CF-1 * DBA/2 Not Specified four-cell embryos
8C E2.5 (a) TS3: 8-cell stage conceptus Absent Not Specified involves: C57BL/6 * CF-1 * DBA/2 Not Specified eight-cell embryos
M E3.0 (a) TS3: morula-stage conceptus Absent Not Specified involves: C57BL/6 * CF-1 * DBA/2 Not Specified morulae
B E3.5 (a) TS4: blastocyst-stage conceptus Absent Not Specified involves: C57BL/6 * CF-1 * DBA/2 Not Specified blastocysts
Lane 12 Mol. Wt. Marker Lane
Notes:
(a) Age assigned by curator based on morphological criteria supplied by authors.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/09/2024
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