Antibody:	beta-Dystroglycan MANDAG-2
Detection system:	Secondary antibody coupled to Alexa Fluor 594
Assay notes:	Fixative was 0.2% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS19: cerebellum primordium	Strong		1A
TS19: forebrain	Strong		1A
TS19: future pituitary	Strong		1A
TS19: hindbrain	Strong		1A
TS19: midbrain	Strong		1A
TS19: surface ectoderm	Strong		1A
TS19: nasal pit epithelium	Strong	Regionally restricted	1A	Expression is in neuroepithelium.

Structure	Level	Pattern	Image	Note
TS15: future brain floor plate	Present	Regionally restricted	1B	Expression is in the basement membrane delineating the neuroepithelium.
TS15: future forebrain	Present	Regionally restricted	1B	Expression is in the basement membrane delineating the neuroepithelium.
TS15: optic vesicle	Present	Regionally restricted	1B	Expression is in the basement membrane delineating the neuroepithelium.
TS15: otocyst	Present	Regionally restricted	1B	Expression is in the basement membrane delineating the neuroepithelium.
TS15: Rathke's pouch	Present		1B

Structure	Level	Pattern	Image	Note
TS15: future brain floor plate	Present	Regionally restricted	1C	Expression is in the basement membrane delineating the neuroepithelium.
TS15: future hindbrain	Present	Regionally restricted	1C	Expression is in the basement membrane delineating the neuroepithelium.
TS15: optic vesicle	Present	Regionally restricted	1C	Expression is in the basement membrane delineating the neuroepithelium.
TS15: Rathke's pouch	Present		1C

Structure	Level	Pattern	Image	Note
TS15: lens placode	Present		1D
TS15: optic cup	Present	Regionally restricted	1D	Expression is in the outer layer.

Structure	Level	Pattern	Image	Note
TS17: lens pit	Present		1E
TS17: optic cup outer layer	Present		1E
TS17: optic stalk	Present		1E

Structure	Level	Pattern	Image	Note
TS19: cornea epithelium	Present		1F
TS19: lens vesicle	Present		1F
TS19: optic stalk	Present		1F
TS19: retina	Present		1F

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	1G	Expression is in the basement membrane.
TS17: neural tube lateral wall	Present	Regionally restricted	1G	Expression is in motoneuron projections.
TS17: notochord	Present		1G
TS17: neural tube floor plate	Present	Regionally restricted	1G	Expression is at the thoracic level extending to the hindbrain, absent in the posterior neural tube.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	1H	Expression is in the basement membrane.
TS15: neural tube lateral wall	Present	Regionally restricted	1H	Expression is in motoneuron projections.
TS15: neural tube floor plate	Present	Regionally restricted	1H	Expression is at the thoracic level extending to the hindbrain, absent in the posterior neural tube.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	1I	Expression is in the basement membrane.
TS17: neural tube lateral wall	Present	Regionally restricted	1I	Expression is in motoneuron projections.
TS17: neural tube floor plate	Present	Regionally restricted	1I	Expression is at the thoracic level extending to the hindbrain, absent in the posterior neural tube.

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present	Regionally restricted	1J	Expression is in the basement membrane.
TS19: neural tube basal columns	Present	Regionally restricted	1J	Expression is in motoneuron projections.
TS19: neural tube floor plate	Present	Regionally restricted	1J	Expression is at the thoracic level extending to the hindbrain, absent in the posterior neural tube.

Structure	Level	Pattern	Image	Note
TS17: hindbrain floor plate	Present		1K
TS17: optic cup	Present		1K

Structure	Level	Pattern	Image	Note
TS15: branchial arch ectoderm	Present		2A
TS15: heart	Present	Regionally restricted	2A	Expression is in the pericardium.
TS15: cardiac muscle tissue	Present	Regionally restricted	2A	Expression is in the trabeculated myocardium.

Structure	Level	Pattern	Image	Note
TS15: foregut	Strong	Regionally restricted	2B	Expression is in the basement membrane.
TS15: cardiac muscle tissue	Present	Regionally restricted	2B	Expression is in the trabeculated myocardium.

Structure	Level	Pattern	Image	Note
TS15: hindgut	Strong	Regionally restricted	2C	Expression is in the basement membrane.

Structure	Level	Pattern	Image	Note
TS15: midgut	Present	Regionally restricted		Expression is in the basement membrane.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch ectoderm	Present		2D
TS17: 2nd branchial arch ectoderm	Present		2D
TS17: 3rd branchial arch ectoderm	Present		2D

Structure	Level	Pattern	Image	Note
TS17: common atrial chamber cardiac muscle	Strong		2E
TS17: foregut	Strong		2E
TS17: primitive ventricle cardiac muscle	Strong		2E
TS17: future spinal cord	Present	Regionally restricted	2E	Expression is in the basement membrane.
TS17: notochord	Present		2E
TS17: endocardial lining	Absent		2E

Structure	Level	Pattern	Image	Note
TS19: ventricle cardiac muscle	Strong	Regionally restricted	2F	Expression is in the trabeculae.
TS19: atrium cardiac muscle	Strong		2F
TS19: stomach epithelium	Strong		2F

Structure	Level	Pattern	Image	Note
TS19: gallbladder	Present		2G
TS19: mesonephric tubule	Present		2G
TS19: nephric duct, mesonephric portion	Present		2G
TS19: stomach epithelium	Present		2G
TS19: urogenital ridge	Present		2G

Structure	Level	Pattern	Image	Note
TS19: atrium cardiac muscle	Present		2H
TS19: main bronchus	Present		2H
TS19: notochord	Present		2H
TS19: ventricle cardiac muscle	Present		2H
TS19: esophagus	Present	Regionally restricted	2H	Expression is in the basement membrane underlying the epithelial lining.
TS19: endocardial lining	Absent		2H

Structure	Level	Pattern	Image	Note
TS19: atrium cardiac muscle	Present		2I
TS19: mesonephric tubule	Present		2I
TS19: nephric duct, mesonephric portion	Present		2I
TS19: notochord	Present		2I
TS19: stomach epithelium	Present		2I
TS19: urogenital ridge	Present		2I
TS19: ventricle cardiac muscle	Present		2I
TS19: future spinal cord	Present	Regionally restricted	2I	Expression is in the basement membrane.

Structure	Level	Pattern	Image	Note
TS19: ventricle cardiac muscle	Present		2I'

Structure	Level	Pattern	Image	Note
TS19: ventricle cardiac muscle	Present		2I' inset	Expression is within the trabeculae and in the trabeculae walls.

Structure	Level	Pattern	Image	Note
TS15: mesonephros	Strong		3A

Structure	Level	Pattern	Image	Note
TS15: mesonephros	Strong		3A'

Structure	Level	Pattern	Image	Note
TS17: mesonephros	Present		3B
TS17: urogenital ridge	Weak		3B

Structure	Level	Pattern	Image	Note
TS17: mesonephros	Present		3B'
TS17: urogenital ridge	Weak		3B'

Structure	Level	Pattern	Image	Note
TS19: mesonephric tubule	Present		3C
TS19: nephric duct, mesonephric portion	Present		3C
TS19: urogenital ridge	Present		3C

Structure	Level	Pattern	Image	Note
TS19: mesonephric tubule	Present		3D
TS19: nephric duct, mesonephric portion	Present		3D
TS19: urogenital ridge	Present		3D

Structure	Level	Pattern	Image	Note
TS15: myotome	Present		4B	Expression is in myotomal cells and associated with the myotomal membrane.

Structure	Level	Pattern	Image	Note
TS15: myotome	Present		4C	Expression is in myotomal cells and associated with the myotomal membrane.

Structure	Level	Pattern	Image	Note
TS15: myotome	Present		4D	Expression is in myotomal cells and associated with the myotomal membrane.

Structure	Level	Pattern	Image	Note
TS15: somite	Weak		4E	Caudal somites.

Structure	Level	Pattern	Image	Note
TS15: myotome	Present	Regionally restricted	4F	Expression is pericellular, absent in the dorsal medial lip.
TS15: future spinal cord	Present	Regionally restricted	4F	Expression is in the basement membrane.

Structure	Level	Pattern	Image	Note
TS15: myotome	Present		4G	Expression is pericellular, absent in the dorsal medial lip.

Structure	Level	Pattern	Image	Note
TS15: somite	Present		4H

Structure	Level	Pattern	Image	Note
TS15: somite	Strong		4I

Structure	Level	Pattern	Image	Note
TS17: myotome	Strong		4J	Expression is also in the basement membrane in the epaxial myotome.

Structure	Level	Pattern	Image	Note
TS17: myotome	Strong		4K	Expression is also in the basement membrane in the epaxial myotome.

Structure	Level	Pattern	Image	Note
TS17: myotome	Present	Regionally restricted	4L	Expression is in the dorsomedial lip and myotomal basement membrane.

Structure	Level	Pattern	Image	Note
TS17: myotome	Present	Regionally restricted	4M	Expression is in the dorsomedial lip and myotomal basement membrane.

Structure	Level	Pattern	Image	Note
TS19: myotome	Present		4N	Expression is in the basement membrane in the epaxial and hypaxial myotome and is down-regulated in the central myotome of hindlimb somites.

Structure	Level	Pattern	Image	Note
TS19: myotome	Present		4O	Expression is in the basement membrane in the epaxial and hypaxial myotome and is down-regulated in the central myotome of hindlimb somites.

Structure	Level	Pattern	Image	Note
TS19: myotome	Present		4P	Expression is in the basement membrane in the epaxial and hypaxial myotome and is down-regulated in the central myotome of hindlimb somites.