Gene Expression Data
Assay Details
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Assay
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Reference:
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J:102271
Ding L, et al., Short-term lineage analysis of dorsally derived Olig3 cells in the developing spinal cord. Dev Dyn. 2005 Nov;234(3):622-32
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5551310
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Gene symbol:
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Olig3
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Gene name:
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oligodendrocyte transcription factor 3
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
2D:
embryonic day 9.25; Olig3tm1Ono/Olig3 +
(more  )
Specimen
2D:
(close  )
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Genetic Background:
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involves: BALB/c * C57BL/6 * C57BL/6NCrj * CBA
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Mutant Allele(s):
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Olig3tm1Ono/Olig3+
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Age:
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embryonic day 9.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future hindbrain |
Present |
Regionally restricted |
2D
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Staining was detected in the paramedian dorsal portion.
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| TS15: future spinal cord |
Present |
Regionally restricted |
2D
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Staining was detected in the paramedian dorsal portion.
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Specimen
4A:
embryonic day 9.5; Olig3tm1Ono/Olig3 +
(more )
Specimen
4A:
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6 * C57BL/6NCrj * CBA
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Mutant Allele(s):
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Olig3tm1Ono/Olig3+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural tube |
Present |
Regionally restricted |
4A
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Staining was intense in the dorsal-most portion of the neural tube.
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| TS15: future spinal cord |
Present |
Regionally restricted |
4A
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A few positive cells were sporadically observed in the subpial portion in the dorsolateral and lateral parts of the spinal cord.
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Specimen
4B:
embryonic day 10.5; Olig3tm1Ono/Olig3 +
(more )
Specimen
4B:
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6 * C57BL/6NCrj * CBA
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Mutant Allele(s):
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Olig3tm1Ono/Olig3+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Present |
Regionally restricted |
4B
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Dense staining was observed in the dorsal-most spinal cord. A dorsal-to-ventral stand of cells forms from the dorsal domain to the ventral-most level. In the strand, dorsal cells are subpial, while ventral cells course medially to the ventral horn.
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Specimen
4C,D:
embryonic day 11.5; Olig3tm1Ono/Olig3 +
(more )
Specimen
4C,D:
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6 * C57BL/6NCrj * CBA
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Mutant Allele(s):
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Olig3tm1Ono/Olig3+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
Present |
Regionally restricted |
4C
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Expression in the dorsal-to-ventral strand of cells was thicker with a higher number of labeled cells at E11.5 compared to E10.5. Positive cells in the most ventral level were positioned just dorsal to the floor plate and lateral to the ventricular zone.
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| TS19: neural tube ventricular layer |
Weak |
Regionally restricted |
4C
,
4D
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Weak staining was detected in cells in the ventral ventricular zone.
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Specimen
4E,F:
embryonic day 12.5; Olig3tm1Ono/Olig3 +
(more )
Specimen
4E,F:
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6 * C57BL/6NCrj * CBA
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Mutant Allele(s):
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Olig3tm1Ono/Olig3+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
4F
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4E
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Positive cells were aligned along the outer edge of the ventricular zone and accumulated in the intermediate level of the spinal cord.
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| TS20: spinal cord roof plate |
Present |
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4E
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Specimen
4G:
embryonic day 14.5; Olig3tm1Ono/Olig3 +
(more )
Specimen
4G:
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6 * C57BL/6NCrj * CBA
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Mutant Allele(s):
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Olig3tm1Ono/Olig3+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord |
Present |
Regionally restricted |
4G
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There were three clusters of staining: the posterior median septum, the mediolateral cell cluster, and the ventromedial cells group.
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Specimen
4H:
embryonic day 18.5; Olig3tm1Ono/Olig3 +
(more )
Specimen
4H:
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6 * C57BL/6NCrj * CBA
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Mutant Allele(s):
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Olig3tm1Ono/Olig3+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: spinal cord |
Present |
Regionally restricted |
4H
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Staining continued in three clusters: the dorsal midline, the intermediate level, and the ventromedial part. However, the number of labeled cells decreased.
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Specimen
4L:
embryonic day 10.5; Olig3tm1Ono/Olig3 +
(more )
Note: Double labeled: light blue - Olig3 reporter; dark blue - Atoh1 (assay MGI:5551388).
Specimen
4L:
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6 * C57BL/6NCrj * CBA
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Mutant Allele(s):
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Olig3tm1Ono/Olig3+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: light blue - Olig3 reporter; dark blue - Atoh1 (assay MGI:5551388).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Present |
Regionally restricted |
4L
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Staining overlapped with expression of Math1 (Atoh1) in the dorsal part of the spinal cord, indicating expression in dorsal interneurons (dI1).
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Specimen
4M:
embryonic day 10.5; Olig3tm1Ono/Olig3 +
(more )
Note: Double labeled: light blue - Olig3 reporter; dark blue - Pou4f1 (assay MGI:5551389).
Specimen
4M:
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6 * C57BL/6NCrj * CBA
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Mutant Allele(s):
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Olig3tm1Ono/Olig3+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: light blue - Olig3 reporter; dark blue - Pou4f1 (assay MGI:5551389).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Present |
Regionally restricted |
4M
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Staining overlapped with expression of Brn3a (Pou4f1) in the dorsal part of the spinal cord, indicating expression in dorsal interneurons (dI1, dI2, dI3).
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