Antibody:	CT-Neog
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS22: neural retinal epithelium	Present	Regionally restricted	1B	Expressed in the retinal ganglion cells and their axons in the nerve fibre layer.
TS22: lens equatorial epithelium	Strong	Regionally restricted	1B	Expressed in the equatorial regions of the developing lens where the anterior capsular epithelial cells are gradually incorporating into the lens proper.
TS22: lens epithelium	Present	Regionally restricted	1B	Expressed in all aspects of the lens epithelium, most highly concentrated on the exterior surface of the epithelium.

Structure	Level	Pattern	Image	Note
TS22: cochlear ganglion	Present		1C
TS22: cochlear duct epithelium	Strong	Regionally restricted	1C	Expressed in the basal surface of the columnar epithelium.

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present	Regionally restricted	1E	Expressed in the cycling secondary progenitors in the basal surface. Not expressed by the primary progenitors at the apical surface.
TS22: telencephalon	Strong	Regionally restricted	1E	Expressed in the lamina propria separating the olfactory epithelium from the border of the olfactory bulb in the rostralmost region.
TS22: nervous system	Present	Regionally restricted	1E	Expressed in large aggregates in the vicinity of the peripheral nervous system-central nervous system transition zone and in the migratory mass abutting the transition zone.

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present	Regionally restricted	1G	Expressed in the cycling secondary progenitors in the basal surface. Not expressed by the primary progenitors at the apical surface.
TS22: telencephalon	Strong	Regionally restricted	1G	Expressed in the lamina propria separating the olfactory epithelium from the border of the olfactory bulb in the rostralmost region.
TS22: nervous system	Present	Regionally restricted	1G	Expressed in large aggregates in the vicinity of the peripheral nervous system-central nervous system transition zone and in the migratory mass abutting the transition zone.

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Present	Non-Uniform	1H	Expressed in fibers projecting from the neurons. Low expression in cell body.
TS22: trigeminal ganglion	Present	Non-Uniform	1H	Expressed in fibers projecting from the neurons. Low expression in cell body.

Structure	Level	Pattern	Image	Note
TS22: trigeminal ganglion	Present	Non-Uniform	1I	Expressed in fibers projecting from the neurons. Low expression in cell body.
TS22: dorsal root ganglion	Present	Non-Uniform	1I	Expressed in fibers projecting from the neurons. Low expression in cell body. Expressed in sensory axons projecting from the dorsal root ganglia.

Structure	Level	Pattern	Image	Note
TS22: midgut epithelium	Strong		2A , 2C	More intense on the basolateral surface.
TS22: midgut	Strong	Regionally restricted	2A , 2C	Expressed in the epithelium and inner circular muscle. Expressed in discrete cells within the submucosa mesenchyme. No expression in the outer longitudinal muscle layer.

Structure	Level	Pattern	Image	Note
TS22: stomach epithelium	Absent		2D
TS22: stomach mesenchyme	Present	Regionally restricted	2D	Expressed in the smooth muscle layer. Expressed in individual cells throughout the stomach mesenchyme.
TS22: pancreas epithelium	Present	Regionally restricted	2D	Expressed in the basolateral surface of the epithelial lining of the pancreatic ducts and on the acinar cells bordering the lumen of the pancreatic buds.
TS22: pancreas mesenchyme	Weak	Regionally restricted	2D	Expressed in the mesenchyme adjacent to the simple epithelia.

Structure	Level	Pattern	Image	Note
TS22: pancreas epithelium	Present	Regionally restricted	2F	Expressed in the basolateral surface of the epithelial lining of the pancreatic ducts and on the acinar cells bordering the lumen of the pancreatic buds.
TS22: pancreas mesenchyme	Weak	Regionally restricted	2F	Expressed in the mesenchyme adjacent to the simple epithelia.

Structure	Level	Pattern	Image	Note
TS22: lung epithelium	Absent		2G , 2H
TS22: lobar bronchus mesenchyme	Present	Regionally restricted	2H , 2G	Expressed in the mesenchymal cells surround the epithelium of the terminal bronchioles and the segmental bronchi.

Structure	Level	Pattern	Image	Note
TS22: mesenchyme of metanephros	Present	Regionally restricted	2J	Expressed in mesenchymal cells surrounding the epithelium of the primitive glomeruli and metanephric tubules.
TS22: adrenal gland	Present	Regionally restricted	2J	Expressed in mesenchymal cells.
TS22: metanephros	Weak		2J

Structure	Level	Pattern	Image	Note
TS22: mesenchyme of metanephros	Present	Regionally restricted	2K	Expressed in mesenchymal cells surrounding the epithelium of the primitive glomeruli and metanephric tubules.

Structure	Level	Pattern	Image	Note
TS22: mandible	Present	Regionally restricted	3A	Expressed in the condensing cartilage at high to intermediate levels.
TS22: maxilla	Present	Regionally restricted	3A	Expressed in the condensing cartilage at high to intermediate levels.
TS22: head mesenchyme	Present	Regionally restricted	3A	Expressed in the condensing cartilage at high to intermediate levels. Also expressed in the surrounding mesenchyme at a lower level.
TS22: nasal mesenchyme	Present	Regionally restricted	3A	Expressed in the condensing cartilage at high to intermediate levels. Also expressed in the surrounding mesenchyme at a lower level.
TS22: nasal septum mesenchyme	Present	Regionally restricted	3A	Expressed in the condensing cartilage at high to intermediate levels. Also expressed in the surrounding mesenchyme at a lower level.

Structure	Level	Pattern	Image	Note
TS22: hindlimb cartilage condensation	Present		3C
TS22: hindlimb musculature	Present		3C

Structure	Level	Pattern	Image	Note
TS22: forelimb cartilage condensation	Present		3D
TS22: forelimb musculature	Present		3D

Structure	Level	Pattern	Image	Note
TS22: mesenteric artery	Present	Regionally restricted	3F	Expressed in smooth muscle cells surrounding the superior mesenteric artery.
TS22: umbilical artery	Present	Regionally restricted	3F	Expressed in smooth muscle cells surrounding the artery.

Structure	Level	Pattern	Image	Note
TS22: cartilage	Present	Widespread		Expressed throughout the embryo.
TS22: musculature	Present	Widespread		Expressed throughout the embryo.
TS22: arterial system	Strong	Regionally restricted		Expressed in vascular smooth muscle cells.
TS22: blood vessel	Strong	Regionally restricted		Expressed in vascular smooth muscle cells.
TS22: cranial muscle	Present
TS22: cranium	Present
TS22: vertebral cartilage condensation	Present