Antibody:	anti-NF200 (N4142)
Detection system:	Secondary antibody coupled to Alexa Fluor 594

Structure	Level	Pattern	Image	Note
TS22: pelvic ganglion	Present		GUDMAP:20939
TS22: nerve of bladder	Present	Graded	GUDMAP:20939	lateral expression; Axons associated with surface (serosa/adventitia) and detrusor are primarily in thick bundles in dorsal aspect and thin bundles/single axons in ventral aspect (detrusor only). Axons in all areas are less prevalent than E16-18. Axons are scarce in lamina propria and not seen close to the urothelium.
TS22: nerve of lamina propria of bladder	Present		GUDMAP:20939	Axons are scarce in lamina propria and not seen close to the urothelium.
TS22: nerve of detrusor muscle of bladder	Present	Graded	GUDMAP:20939	dorsal expression; Axons associated with detrusor are primarily in thick bundles in dorsal aspect and thin bundles/single axons in ventral aspect.
TS22: nerve of outer layer of bladder	Present	Graded	GUDMAP:20939	dorsal expression; Axons associated with surface (serosa/adventitia) are primarily in thick bundles in dorsal aspect.
TS22: nerve of urothelium of bladder	Absent		GUDMAP:20939

Structure	Level	Pattern	Image	Note
TS26: pelvic ganglion	Present		GUDMAP:20943	Expression only in axons, not neuronal cell bodies.
TS26: nerve of urethra	Present		GUDMAP:20943
TS26: nerve of urothelium of bladder	Present		GUDMAP:20943
TS26: nerve of lamina propria of bladder	Present		GUDMAP:20943
TS26: nerve of detrusor muscle of bladder	Present	Graded	GUDMAP:20943	dorsal expression; Thicker bundles of axons more prevalent in dorsal tissue; thinner bundles/single axons more prevalent in ventral tissue.
TS26: nerve of outer layer of bladder	Present	Graded	GUDMAP:20943	dorsal expression; Thicker bundles of axons more prevalent in dorsal tissue; thinner bundles/single axons more prevalent in ventral tissue.

Structure	Level	Pattern	Image	Note
TS24: nerve of bladder	Present		GUDMAP:21003
TS24: nerve of lamina propria of bladder	Present		GUDMAP:21003
TS24: nerve of detrusor muscle of bladder	Present	Graded	GUDMAP:21003	Higher density of axons (especially thick axon bundles) on dorsal vs ventral aspect of detrusor.
TS24: nerve of outer layer of bladder	Present	Graded	GUDMAP:21003
TS24: nerve of urothelium of bladder	Present		GUDMAP:21003	Suburothelial axons are scarce