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Gt(ROSA)26Sortm9(EGFP/Rpl10a)Amc
Targeted Allele Detail
Summary
Symbol: Gt(ROSA)26Sortm9(EGFP/Rpl10a)Amc
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 9, Andrew P McMahon
MGI ID: MGI:5548082
Synonyms: EGFP-L10a, L10a, L10A-EGFP, R26Trap
Gene: Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance: Gt(ROSA)26Sortm9(EGFP/Rpl10a)Amc page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:206130
Parent Cell Line:  v6.5 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Mutation
description
Allele Type:    Targeted (Conditional ready, Reporter)
Mutation:    Insertion
 
Mutation details: A recombinase mediated exchange (RMCE) vector was designed to contain (from 5' to 3') a promoterless neomycin cassette followed by 3 SV40 polyadenylation sites (pA), 3xpA, and in reverse orientation a fragment containing 3xIns (400 bp core region of chicken beta-globin insulator), CAGGS (CMV-IE enhancer/chicken beta-actin/rabbit beta-globin) hybrid promoter, floxed-3xpA, and a cDNA encoding an enhanced green fluorescent protein (EGFP) fused to ribosomal protein unit L10a (Rpl10a). The loxP-flanked pA cassette is designed to block transcription of EGFP/Rpl10a. (J:206130)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  942 strains or lines available
Notes
The V6.5 embryonic stem (ES) cells used in the making of the mouse were targeted with a GT(ROSA)26Sor targeting vector containing an F3- and FRT-flanked ATG-F3-Puro-pA cassette followed by a PGK promoter driving expression of FLPo recombinase.
References
Original:  J:206130 Liu J, et al., Cell-specific translational profiling in acute kidney injury. J Clin Invest. 2014 Mar 3;124(3):1242-54
All:  37 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
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Send questions and comments to User Support. 		last database update
04/16/2024
MGI 6.23 		The Jackson Laboratory