Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:125514
Hartman BH, et al., Dll3 is expressed in developing hair cells in the mammalian cochlea. Dev Dyn. 2007 Oct;236(10):2875-83
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:5547528
|
Gene symbol:
|
Jag2
|
Gene name:
|
jagged 2
|
Probe:
|
MGC clone 11909
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
Assay notes:
|
Modified Carnoy's fixative: 60% ethanol/30% formaldehyde/10% acetic acid
|
|
Results
|
Specimen
1G,H,I:
embryonic day 15.5
(more )
Specimen
1G,H,I:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Carnoy's Fixative
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: cochlea |
Present |
Regionally restricted |
1I
,
1G
,
1H
|
Expression was detected in the presumptive inner hair cells in the basal turn (I) and the mid-basal turn (H).
|
TS23: cochlea epithelium |
Present |
Regionally restricted |
1H
,
1G
|
Expression was observed in columns extending through the thickness of the epithelium.
|
Specimen
3M,N:
embryonic day 16.5
(more )
Specimen
3M,N:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Carnoy's Fixative
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: cochlea |
Present |
Regionally restricted |
3M
,
3N
|
Expression was detected at a moderate level in inner hair cells and at a stronger level in outer hair cells. There was strong expression in the basal turns and low level expression in the middle turn.
|
|