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Gene Expression Data
Assay Details
Assay
Reference: J:97081 Lees-Murdock DJ, et al., DNA methyltransferase expression in the mouse germ line during periods of de novo methylation. Dev Dyn. 2005 Apr;232(4):992-1002
Assay type: RT-PCR
MGI Accession ID: MGI:5545797
Gene symbol: Dnmt3a
Gene name: DNA methyltransferase 3A
Probe: Dnmt3a2-F5, Dnmt3a-R5
Assay notes: This blot contains the results of a multiplex reaction; other bands, not produced by this primer set, are present on the blot.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
- Mol. Wt. Marker Lane
germ line e12.5 E12.5 TS20: germ cell of ovary
TS20: ovary 		Present 1 µg; total RNA HsdOla:TO Female Germ cells were purified without culture (purity 85%).
germ line e15.5 E15.5 TS23: germ cell of ovary
TS23: ovary 		Present 1 µg; total RNA HsdOla:TO Female Germ cells were purified without culture (purity 85%).
germ line e17.5 E17.5 TS25: germ cell of ovary
TS25: ovary 		Present 1 µg; total RNA HsdOla:TO Female Germ cells were purified without culture (purity 85%).
germ line 12dpp adult P12 TS28: ovary Present 1 µg; total RNA HsdOla:TO Female
germ line 24dpp P24 TS28: ovary Present 1 µg; total RNA HsdOla:TO Female
germ line adult P adult TS28: ovary Present 1 µg; total RNA HsdOla:TO Female
soma e8.5 embryo E8.5 TS13: embryo Present 1 µg; total RNA HsdOla:TO Female
soma lung P adult TS28: lung Weak 1 µg; total RNA HsdOla:TO Female
soma spleen P adult TS28: spleen Weak 1 µg; total RNA HsdOla:TO Female
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory