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Gene Expression Data
Assay Details
Assay
Reference: J:97081 Lees-Murdock DJ, et al., DNA methyltransferase expression in the mouse germ line during periods of de novo methylation. Dev Dyn. 2005 Apr;232(4):992-1002
Assay type: RT-PCR
MGI Accession ID: MGI:5545791
Gene symbol: Dnmt3a
Gene name: DNA methyltransferase 3A
Probe: Dnmt3a-F9, Dnmt3a-R5
Assay notes: This blot contains the results of a multiplex reaction; other bands, not produced by this primer set, are present on the blot.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
- Mol. Wt. Marker Lane
germ line e12.5 E12.5 TS20: germ cell of testis
TS20: testis 		Present 1 µg; total RNA HsdOla:TO Male Germ cells were purified without culture (purity 85%).
germ line e15.5 E15.5 TS23: germ cell of testis
TS23: testis 		Present 1 µg; total RNA HsdOla:TO Male Germ cells were purified without culture (purity 85%).
germ line e17.5 E17.5 TS25: germ cell of testis
TS25: testis 		Present 1 µg; total RNA HsdOla:TO Male Germ cells were purified without culture (purity 85%).
germ line 12dpp adult P12 TS28: testis Present 1 µg; total RNA HsdOla:TO Male
germ line 24dpp P24 TS28: testis Present 1 µg; total RNA HsdOla:TO Male
germ line adult P adult TS28: testis Present 1 µg; total RNA HsdOla:TO Male
soma e8.5 embryo E8.5 TS13: embryo Ambiguous (a) 1 µg; total RNA HsdOla:TO Male
soma lung P adult TS28: lung Present 1 µg; total RNA HsdOla:TO Male
soma spleen P adult TS28: spleen Present 1 µg; total RNA HsdOla:TO Male
Notes:
(a) Undetectable.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory