Probe:	Six9 probe1
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	Vibratome sections.

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Strong	Regionally restricted	2b	Strong in the ventral suprachiasmatic nucleus.
TS22: thalamus	Present	Regionally restricted	2b	Expression is in the ventral thalamus.

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Strong	Regionally restricted	2d	Strong in the ventral suprachiasmatic nucleus.

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Strong	Regionally restricted	2f	Strong in the ventral suprachiasmatic nucleus, expanding caudally into the dorsomedial and ventromedial hypothalamic regions.

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Strong	Regionally restricted	2h	Strong in the ventral suprachiasmatic nucleus, expanding caudally into the dorsomedial and ventromedial hypothalamic regions as a stream of dorsally migrating cells.
TS22: thalamus	Weak	Regionally restricted	2h	Expression is ventral.

Structure	Level	Pattern	Image	Note
TS25: hypothalamus	Present	Regionally restricted	3b	Expression is in the most anterior and ventral portion of the hypothalamus corresponding to the suprachiasmatic nucleus and in the paraventricular, anterodorsal, and anterobasal hypothalamic nuclei.

Structure	Level	Pattern	Image	Note
TS25: hypothalamus	Present	Regionally restricted	3d	Expression is in the most anterior and ventral portion of the hypothalamus corresponding to the suprachiasmatic nucleus and in the paraventricular, anterodorsal, and anterobasal hypothalamic nuclei.
TS25: thalamus	Present		3d	Expression is in the reticular thalamic nucleus.

Structure	Level	Pattern	Image	Note
TS27: hypothalamus	Present		3f
TS27: reticular thalamic nucleus	Present		3f
TS27: suprachiasmatic nucleus	Present		3f
TS27: zona incerta	Present		3f

Structure	Level	Pattern	Image	Note
TS27: paraventricular hypothalamic nucleus	Present		3h
TS27: reticular thalamic nucleus	Present		3h
TS27: suprachiasmatic nucleus	Present		3h
TS27: zona incerta	Present		3h
TS27: hypothalamus	Present	Regionally restricted	3h	Expression is in the most anterior and ventral portion of the hypothalamus corresponding to the suprachiasmatic nucleus and in the paraventricular, anterodorsal, and anterobasal hypothalamic nuclei.

Structure	Level	Pattern	Image	Note
TS28: hypothalamus	Present	Regionally restricted	5b	Expression is in the most anterior and ventral portion of the hypothalamus corresponding to the suprachiasmatic nucleus and in the paraventricular, anterodorsal, and anterobasal hypothalamic nuclei.
TS28: paraventricular hypothalamic nucleus	Present		5b
TS28: suprachiasmatic nucleus	Present		5b

Structure	Level	Pattern	Image	Note
TS28: hypothalamus	Weak	Regionally restricted	5d	Expression is in the most anterior and ventral portion of the hypothalamus corresponding to the suprachiasmatic nucleus and in the paraventricular, anterodorsal, and anterobasal hypothalamic nuclei.
TS28: paraventricular hypothalamic nucleus	Weak		5d
TS28: suprachiasmatic nucleus	Weak		5d
TS28: zona incerta	Weak		5d
TS28: reticular thalamic nucleus	Present		5d

Structure	Level	Pattern	Image	Note
TS28: suprachiasmatic nucleus	Moderate		5f
TS28: reticular thalamic nucleus	Weak		5f
TS28: zona incerta	Weak		5f