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Gene Expression Data
Assay Details
Assay
Reference: J:97081 Lees-Murdock DJ, et al., DNA methyltransferase expression in the mouse germ line during periods of de novo methylation. Dev Dyn. 2005 Apr;232(4):992-1002
Assay type: RT-PCR
MGI Accession ID: MGI:5545654
Gene symbol: Dnmt3a
Gene name: DNA methyltransferase 3A
Probe: Dnmt3a-F1, Dnmt3a-R1
Assay notes: This blot contains the results of a multiplex reaction; other bands are for beta-actin as control. Quantification of these results is reported in Table 1.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure 383 bp (a) Amount Genetic BackgroundMutant Allele(s)Sex Note
- Mol. Wt. Marker Lane
- Lane not used in this assay
Male e12.5 E12.5 TS20: germ cell of testis
TS20: testis 		Present 1 µg; total RNA HsdOla:TO Male Germ cells were purified without culture (purity 85%).
Male e17.5 E17.5 TS25: germ cell of testis
TS25: testis 		Present 1 µg; total RNA HsdOla:TO Male Germ cells were purified without culture (purity 85%).
Male adult P adult TS28: testis Present 1 µg; total RNA HsdOla:TO Male
Female e12.5 E12.5 TS20: germ cell of ovary
TS20: ovary 		Present 1 µg; total RNA HsdOla:TO Female Germ cells were purified without culture (purity 85%).
Female e17.5 E17.5 TS25: germ cell of ovary
TS25: ovary 		Present 1 µg; total RNA HsdOla:TO Female Germ cells were purified without culture (purity 85%).
Female adult P adult TS28: ovary Present 1 µg; total RNA HsdOla:TO Female
lung P adult TS28: lung Weak 1 µg; total RNA HsdOla:TO Not Specified
e8.5 embryo E8.5 TS13: embryo Strong 1 µg; total RNA HsdOla:TO Not Specified
- Lane not used in this assay
- Mol. Wt. Marker Lane
Notes:
(a) Transcripts were detected at uniform levels at all stages.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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