Il2rgtm2.1Vlcg
Targeted Allele Detail
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| Symbol: |
Il2rgtm2.1Vlcg |
| Name: |
interleukin 2 receptor, gamma chain; targeted mutation 2.1, Velocigene |
| MGI ID: |
MGI:5544783 |
| Synonyms: |
Il2rge1REVCOIN |
| Gene: |
Il2rg Location: ChrX:100307991-100311861 bp, - strand Genetic Position: ChrX, 43.9 cM
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| Alliance: |
Il2rgtm2.1Vlcg page
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| Allele Type: |
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Targeted (Conditional ready, No functional change) |
| Mutation: |
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Insertion
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Mutation details: A conditional by inversion (COIN) intron consisting of a reverse orientation lox66_SA-Egpf-polyA_lox71 was inserted in the antisense orientation into exon 1 via homologous recombination, splitting exon 1 into 2 new exons (89 and 112 bp in length). The two lox sites are in reverse complementation orientation, thus cre-mediated recombination will irreversibly invert the inserted sequence and rescue endogenous expression. In addition, an FRT flanked drug selection cassette (hygromycin- or neomycin-delta-thymidine kinase cassette was inserted after the COIN intron. Flp-mediated recombination removed the drug selection cassette.
(J:200671)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Il2rg Mutation: |
175 strains or lines available
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| Original: |
J:200671 Economides AN, et al., Conditionals by inversion provide a universal method for the generation of conditional alleles. Proc Natl Acad Sci U S A. 2013 Aug 20;110(34):E3179-88 |
| All: |
1 reference(s) |
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Analysis Tools
