Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Il2rgtm2.1Vlcg
Name:	interleukin 2 receptor, gamma chain; targeted mutation 2.1, Velocigene
MGI ID:	MGI:5544783
Synonyms:	Il2rge1REVCOIN
Gene:	Il2rg  Location: ChrX:100307991-100311861 bp, - strand  Genetic Position: ChrX, 43.9 cM
Alliance:	Il2rgtm2.1Vlcg page

Germline Transmission:	Earliest citation of germline transmission: J:200671
Parent Cell Line:	F1H4 (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6NTac)F1

Allele Type:    Targeted (Conditional ready, No functional change) Mutation:    Insertion   Mutation details: A conditional by inversion (COIN) intron consisting of a reverse orientation lox66_SA-Egpf-polyA_lox71 was inserted in the antisense orientation into exon 1 via homologous recombination, splitting exon 1 into 2 new exons (89 and 112 bp in length). The two lox sites are in reverse complementation orientation, thus cre-mediated recombination will irreversibly invert the inserted sequence and rescue endogenous expression. In addition, an FRT flanked drug selection cassette (hygromycin- or neomycin-delta-thymidine kinase cassette was inserted after the COIN intron. Flp-mediated recombination removed the drug selection cassette. (J:200671)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Il2rg Mutation:	168 strains or lines available

Original:	J:200671 Economides AN, et al., Conditionals by inversion provide a universal method for the generation of conditional alleles. Proc Natl Acad Sci U S A. 2013 Aug 20;110(34):E3179-88
All:	1 reference(s)