Probe:	Zac1 cDNA5
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: central nervous system	Present	Widespread	1A	Expression was detected in a regionalized pattern throughout the neural tube.
TS15: diencephalon	Present	Regionally restricted	1A	Higher expression levels were observed dorsally.
TS15: future midbrain	Present	Regionally restricted	1A	Higher expression levels were observed dorsally.
TS15: telencephalon	Present	Regionally restricted	1A	Higher expression levels were observed dorsally.
TS15: future hindbrain	Present	Regionally restricted	1A	Higher expression levels were observed ventrally in the hindbrain.
TS15: future spinal cord	Present	Regionally restricted	1A	Higher expression levels were observed dorsally in the caudal spinal cord.
TS15: branchial arch mesenchyme derived from neural crest	Present		1A
TS15: pharynx	Present		1A
TS15: nose	Present	Regionally restricted	1A	Expression was detected in the nasal cavities.
TS15: oral region	Present	Regionally restricted	1A	Expression was detected in the mouth.
TS15: embryo	Present	Regionally restricted	1A	Expression was detected in the larynx and neck.
TS15: heart atrium	Present		1A
TS15: outflow tract	Present		1A
TS15: primitive ventricle	Present		1A
TS15: trunk	Present	Regionally restricted	1A	Expression was detected in the intersomitic regions.
TS15: gut	Present	Regionally restricted	1A	Expression was detected in the lining of the developing gut and stomach.
TS15: forelimb bud	Present	Regionally restricted	1A	Expression was detected in the center of the forelimb bud.
TS15: optic cup	Present		1A

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	1D	Higher expression levels were observed dorsally.
TS15: future midbrain	Present	Regionally restricted	1D	Higher expression levels were observed dorsally.
TS15: telencephalon	Present	Regionally restricted	1D	Higher expression levels were observed dorsally.
TS15: future hindbrain	Present	Regionally restricted	1D	Higher expression levels were observed ventrally in the hindbrain.
TS15: olfactory placode	Present		1D

Structure	Level	Pattern	Image	Note
TS15: future spinal cord	Present	Regionally restricted	1G	Higher expression levels were observed dorsally in the caudal spinal cord.
TS15: trunk	Present	Regionally restricted	1G	Expression was detected in the intersomitic regions.
TS15: gut	Present	Regionally restricted	1G	Expression was detected in the lining of the developing gut and stomach.
TS15: forelimb bud	Present	Regionally restricted	1G	Expression was detected in the center of the forelimb bud.

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Present	Widespread	2A	Expression was detected in a regionalized pattern throughout the neural tube.
TS17: diencephalon	Present	Regionally restricted	2A	Higher expression levels were observed dorsally.
TS17: midbrain	Present	Regionally restricted	2A	Higher expression levels were observed dorsally.
TS17: telencephalon	Present	Regionally restricted	2A	Higher expression levels were observed dorsally.
TS17: hindbrain	Present	Regionally restricted	2A	Higher expression levels were observed ventrally in the hindbrain.
TS17: future spinal cord	Present	Regionally restricted	2A	Higher expression levels were observed dorsally in the caudal spinal cord.
TS17: branchial arch mesenchyme derived from neural crest	Present		2A
TS17: optic cup	Present		2A
TS17: pharynx	Present		2A
TS17: oral region	Present	Regionally restricted	2A	Expression was detected in the mouth.
TS17: embryo	Present	Regionally restricted	2A	Expression was detected in the larynx and neck.
TS17: heart atrium	Present		2A
TS17: outflow tract	Present		2A
TS17: primitive ventricle	Present		2A
TS17: somite	Present	Regionally restricted	2A	Expression was high in the rostral compartments of the somites.
TS17: gut	Present	Regionally restricted	2A	Expression was detected in the lining of the gut and stomach.
TS17: stomach	Present	Regionally restricted	2A	Expression was detected in the lining of the gut and stomach.
TS17: forelimb bud	Present	Regionally restricted	2A	Expression was detected in the center of the forelimb bud.

Structure	Level	Pattern	Image	Note
TS17: branchial arch mesenchyme derived from neural crest	Present		2D
TS17: head mesenchyme	Present	Regionally restricted	2D	Expression was restricted to the non-neural mesenchyme surrounding the olfactory pit.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	2G	Higher expression levels were observed dorsally.
TS17: midbrain	Present	Regionally restricted	2G	Higher expression levels were observed dorsally.
TS17: telencephalon	Present	Regionally restricted	2G	Higher expression levels were observed dorsally.
TS17: hindbrain	Present	Regionally restricted	2G	Higher expression levels were observed ventrally in the hindbrain.
TS17: optic cup	Present		2G

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	2J	Higher expression levels were observed dorsally in the caudal spinal cord.
TS17: heart atrium	Present		2J
TS17: outflow tract	Present		2J
TS17: primitive ventricle	Present		2J
TS17: somite	Present	Regionally restricted	2J	Expression was high in the rostral compartments of the somites.
TS17: forelimb bud	Present	Regionally restricted	2J	Expression was detected in the center of the forelimb bud.

Structure	Level	Pattern	Image	Note
TS17: bulbus cordis	Present		2M
TS17: heart atrium	Present		2M
TS17: stomach	Present	Regionally restricted	2M	Expression was detected in the lining of the stomach.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Strong		3A	Expression was detected in dividing cortical progenitors.
TS20: cortical preplate	Present	Scattered	3A
TS20: cortical hem	Absent		3A	Expression was excluded from the cortical hem.
TS20: lateral ganglionic eminence	Present	Regionally restricted	3A	Expression was detected in the dorsal part of the lateral ganglionic eminence.
TS20: medial ganglionic eminence	Absent		3A

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex ventricular layer	Strong	Regionally restricted	3J	High expression was detected in progenitor cells in the ventricular zone.
TS23: cerebral cortex subventricular zone	Weak	Regionally restricted	3J	Much reduced expression was detected in progenitor cells in the subventricular zone.
TS23: cortical plate	Moderate	Regionally restricted	3J	Intermediate expression was detected in progenitor cells in a band of cells in the cortical plate.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	3M	High expression was detected in progenitor cells of the cortical ventricular zone, at much reduced levels in the subventricular zone, and at intermediate levels in a band of cells in the cortical plate.

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Present		4A' , 4A
TS27: cerebellum internal granule cell layer	Present	Regionally restricted	4A , 4A'	Expression was detected in the internal granular cell layer in posterior lobes.
TS27: cerebellum posterior lobe	Present	Regionally restricted	4A , 4A'	Expression was detected in the internal granular cell layer in posterior lobes.

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Present		4E' , 4E
TS28: cerebellum internal granule cell layer	Present	Regionally restricted	4E , 4E'	Expression was detected in the internal granular cell layer in posterior lobes.
TS28: cerebellum posterior lobe	Present	Regionally restricted	4E , 4E'	Expression was detected in the internal granular cell layer in posterior lobes.

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Present		4I
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	4I	Expression was detected in a subset of Purkinje cells in posterior lobes.
TS28: cerebellum posterior lobe	Present	Regionally restricted	4I	Expression was detected in a subset of Purkinje cells in posterior lobes.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	5A	Expression was high in a broad band of ventricular zone precursors in the dorsal half of the neural tube. A much smaller band of expression was detected in the ventral-most region. Expression overlapped with Mash1 in the dorsal spinal cord.
TS17: neural tube floor plate	Present		5A

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventricular layer	Present	Regionally restricted	5F	Expression was detected in the dorsal ventricular zone. Expression overlapped with Mash1 in the dorsal spinal cord.
TS20: spinal cord mantle layer	Absent		5F	Expression was excluded from postmitotic neurons in the mantle zone.
TS20: dorsal root ganglion	Absent		5F
TS20: sympathetic ganglion	Absent		5F

Structure	Level	Pattern	Image	Note
TS20: neural retinal epithelium	Present	Regionally restricted	6A	Expression was detected in dividing progenitor cells in the outer neuroblast layer of the retina. Expression was excluded from postmitotic precursors and differentiated cells in the inner neuroblast layer.

Structure	Level	Pattern	Image	Note
TS23: retina nuclear layer	Present	Regionally restricted	6D	Expression was detected in dividing progenitor cells in the outer neuroblast layer of the retina. Expression was excluded from postmitotic precursors and differentiated cells in the inner neuroblast layer.

Structure	Level	Pattern	Image	Note
TS27: retina outer nuclear layer	Present	Regionally restricted	6G	Expression was detected in dividing progenitor cells in the outer neuroblast layer of the retina. Expression was excluded from postmitotic precursors.
TS27: retina inner nuclear layer	Absent		6G

Structure	Level	Pattern	Image	Note
TS28: retina outer nuclear layer	Absent		6J
TS28: retina inner nuclear layer	Present	Regionally restricted	6J	Expression was detected in a subset of cells in the inner nuclear layer.
TS28: retina ganglion cell layer	Present	Regionally restricted	6J	Expression was detected in a subset of cells in the ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS28: retina outer nuclear layer	Absent		6M
TS28: retina inner nuclear layer	Present	Regionally restricted	6M	Expression was detected in a subset of cells in the inner nuclear layer.
TS28: retina ganglion cell layer	Present	Regionally restricted	6M	Expression was detected in a subset of cells in the ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS20: olfactory epithelium	Absent		7A
TS20: oral region	Present	Regionally restricted	7A	Expression was detected in the lamina propria underlying the olfactory epithelium.

Structure	Level	Pattern	Image	Note
TS23: oral lamina propria	Present	Regionally restricted	7F	Expression was detected in the lamina propria surrounding the olfactory epithelium.

Structure	Level	Pattern	Image	Note
TS20: pancreas	Present	Regionally restricted	8A	Expression was detected in exocrine pancreas.
TS20: midgut mesenchyme	Present	Regionally restricted	8A	Expression was high in the outer mesenchyme, but was excluded from the presumptive myenteric plexus.

Structure	Level	Pattern	Image	Note
TS20: midgut mesenchyme	Present	Regionally restricted	8F	Expression was high in the outer mesenchyme, but was excluded from the presumptive myenteric plexus.

Structure	Level	Pattern	Image	Note
TS20: pancreas	Present	Regionally restricted	8J	Expression was detected in exocrine pancreas.

Structure	Level	Pattern	Image	Note
TS17: trigeminal ganglion	Present	Scattered
TS17: acoustic ganglion	Absent
TS17: facial ganglion	Absent
TS17: glossopharyngeal ganglion	Absent
TS17: vagus ganglion	Absent