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Gene Expression Data
Assay Details
Assay
Reference: J:171409 GUDMAP Consortium, GUDMAP: the GenitoUrinary Development Molecular Anatomy Project. www.gudmap.org. 2004;
Assay type: RNA in situ
MGI Accession ID: MGI:5541693
Gene symbol: Tmem45a
Gene name: transmembrane protein 45a
Probe: RIKEN clone C130020A02 subclone
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen GUDMAP:10012: embryonic day 10.5 (more )
Note: Labeling was visualized using NBT/BCIP.
Structure Level Pattern Image Note
TS17: genitourinary system Present GUDMAP:10012
TS17: genitourinary system mesenchyme Present GUDMAP:10012
TS17: mesonephros Present Regionally restricted GUDMAP:10012
TS17: mesonephros mesenchyme Present GUDMAP:10012
TS17: mesonephric tubule Absent GUDMAP:10012
TS17: nephric duct, mesonephric portion Absent GUDMAP:10012
TS17: nephric duct, metanephric portion Absent GUDMAP:10012
TS17: metanephric mesenchyme Ambiguous GUDMAP:10012 expression uncertain

Specimen GUDMAP:10338: embryonic day 13.5 (more )
Note: Labeling was visualized using NBT/BCIP.
Structure Level Pattern Image Note
TS21: male reproductive system Present GUDMAP:10338
TS21: mesonephros of male Present Regionally restricted GUDMAP:10338
TS21: mesonephric mesenchyme of male Present GUDMAP:10338
TS21: testis Present Ubiquitous GUDMAP:10338 stronger expression seen in the interstitium
TS21: interstitium of the testis Present GUDMAP:10338

Specimen GUDMAP:10339: embryonic day 13.5 (more )
Note: Labeling was visualized using NBT/BCIP.
Structure Level Pattern Image Note
TS21: female reproductive system Present GUDMAP:10339
TS21: mesonephros of female Present Regionally restricted GUDMAP:10339
TS21: ovary Present Ubiquitous GUDMAP:10339

Specimen GUDMAP:10340: embryonic day 13.5 (more )
Note: Labeling was visualized using NBT/BCIP. Male and female were examined; only the female is shown. Annotators reported the male expression pattern in the metanephros is identical to female.
Structure Level Pattern Image Note
TS21: urinary system Present GUDMAP:10340
TS21: metanephros Present Regionally restricted GUDMAP:10340
TS21: renal interstitium Present GUDMAP:10340
TS21: ureter Present GUDMAP:10340

Specimen GUDMAP:10341: embryonic day 13.5 (more )
Note: Labeling was visualized using NBT/BCIP. Male and female were examined; only the female is shown. Annotators reported the male expression pattern in the urogenital sinus is identical to female.
Structure Level Pattern Image Note
TS21: urinary system Present GUDMAP:10341
TS21: ureter Present GUDMAP:10341
TS21: urogenital sinus Present GUDMAP:10341
TS21: primitive bladder Present GUDMAP:10341 strong expression

Specimen GUDMAP:10769: embryonic day 15.5 (more )
Note: Labeling was visualized using BM purple. The low level expression control (Shh) probe's color developed for 140 hrs, but no expression was detected. Thus, it is possible that very weak levels of gene expression were not detectable in this experiment.
Structure Level Pattern Image Note
TS23: metanephros Absent GUDMAP:10769
TS23: ureter Absent GUDMAP:10769

Specimen GUDMAP:10770: embryonic day 15.5 (more )
Note: Labeling was visualized using BM purple. The low level expression control (Shh) probe's color developed for 140 hrs, but no expression was detected. Thus, it is possible that very weak levels of gene expression were not detectable in this experiment.
Structure Level Pattern Image Note
TS23: ureter Absent GUDMAP:10770


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/14/2024
MGI 6.23
The Jackson Laboratory