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Gene Expression Data
Assay Details
Assay
Reference: J:171409 GUDMAP Consortium, GUDMAP: the GenitoUrinary Development Molecular Anatomy Project. www.gudmap.org. 2004;
Assay type: RNA in situ
MGI Accession ID: MGI:5540892
Gene symbol: Tgfbi
Gene name: transforming growth factor, beta induced
Probe: RIKEN clone D230045E03 subclone
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen GUDMAP:7934: embryonic day 12.5 (more )
Note: Labeling was visualized using NBT/BCIP.
Structure Level Pattern Image Note
TS20: female reproductive system Present GUDMAP:7934
TS20: ovary Present Regionally restricted GUDMAP:7934 rostral and caudal expression; signal restricted to rostral and caudal tips only
TS20: mesonephros of female Present GUDMAP:7934
TS20: mesonephric mesenchyme of female Present GUDMAP:7934
TS20: mesonephric tubule of female Absent GUDMAP:7934
TS20: nephric duct of female, mesonephric portion Ambiguous GUDMAP:7934 signal is weaker than in adjacent mesenchyme and may represent expression in the overlying mesenchyme
TS20: paramesonephric duct of female, mesonephric portion Ambiguous GUDMAP:7934 signal absent from duct epithelium; there may be signal in the duct mesenchyme

Specimen GUDMAP:7935: embryonic day 12.5 (more )
Note: Labeling was visualized using NBT/BCIP.
Structure Level Pattern Image Note
TS20: male reproductive system Present GUDMAP:7935
TS20: testis Present GUDMAP:7935 weak ubiquitous signal with stronger signal at the rostral and caudal tips
TS20: mesonephros of male Present GUDMAP:7935
TS20: mesonephric mesenchyme of male Present GUDMAP:7935
TS20: mesonephric tubule of male Absent GUDMAP:7935
TS20: nephric duct of male, mesonephric portion Ambiguous GUDMAP:7935 signal is weaker than in adjacent mesenchyme and may represent expression in the overlying mesenchyme
TS20: paramesonephric duct of male, mesonephric portion Ambiguous GUDMAP:7935 signal absent from duct epithelium; there may be signal in the duct mesenchyme

Specimen GUDMAP:7936: embryonic day 12.5 (more )
Note: Labeling was visualized using NBT/BCIP.
Structure Level Pattern Image Note
TS20: urinary system Present GUDMAP:7936
TS20: metanephros Present Regionally restricted GUDMAP:7936
TS20: renal interstitium Present GUDMAP:7936
TS20: urogenital mesentery Present GUDMAP:7936
TS20: ureteric tip Absent GUDMAP:7936
TS20: cap mesenchyme Absent GUDMAP:7936
TS20: early nephron Absent GUDMAP:7936

Specimen GUDMAP:7937: embryonic day 12.5 (more )
Note: Labeling was visualized using NBT/BCIP.
Structure Level Pattern Image Note
TS20: urinary system Present GUDMAP:7937
TS20: metanephros Present Regionally restricted GUDMAP:7937
TS20: renal interstitium Present GUDMAP:7937
TS20: urogenital mesentery Present GUDMAP:7937
TS20: primitive bladder Present GUDMAP:7937 signal is weak when compared with the mesonephros

Specimen GUDMAP:7938: embryonic day 10.5 (more )
Note: Labeling was visualized using NBT/BCIP.
Structure Level Pattern Image Note
TS17: genitourinary system Present GUDMAP:7938
TS17: metanephric mesenchyme Present GUDMAP:7938
TS17: nephric duct, metanephric portion Present Regionally restricted GUDMAP:7938 signal restricted to the lumen of the duct
TS17: nephric duct, mesonephric portion Present GUDMAP:7938 signal may be in the duct epithelium or the surrounding mesenchyme
TS17: mesonephros Present GUDMAP:7938
TS17: mesonephros mesenchyme Present GUDMAP:7938
TS17: mesonephric tubule Ambiguous GUDMAP:7938
TS17: cranial mesonephric tubule Ambiguous GUDMAP:7938
TS17: rest of cranial mesonephric tubule Ambiguous GUDMAP:7938
TS17: caudal mesonephric tubule Absent GUDMAP:7938

Specimen GUDMAP:9663: embryonic day 10.5 (more )
Note: Labeling was visualized using NBT/BCIP.
Structure Level Pattern Image Note
TS17: genitourinary system Present Regionally restricted GUDMAP:9663
TS17: genitourinary system mesenchyme Present GUDMAP:9663
TS17: mesonephros Present Regionally restricted GUDMAP:9663
TS17: mesonephros mesenchyme Present GUDMAP:9663
TS17: metanephric mesenchyme Present GUDMAP:9663
TS17: mesonephric tubule Absent GUDMAP:9663
TS17: nephric duct, metanephric portion Absent GUDMAP:9663

Specimen GUDMAP:9664: embryonic day 13.5 (more )
Note: Labeling was visualized using NBT/BCIP.
Structure Level Pattern Image Note
TS21: male reproductive system Present Regionally restricted GUDMAP:9664
TS21: mesonephros of male Present Regionally restricted GUDMAP:9664
TS21: mesonephric mesenchyme of male Present GUDMAP:9664
TS21: nephric duct of male, mesonephric portion Present GUDMAP:9664 strong expression in the mesenchyme surrounding duct
TS21: nephric duct of male, rest of Present GUDMAP:9664
TS21: mesenchyme of nephric duct of male Present GUDMAP:9664
TS21: primary sex cord Absent GUDMAP:9664
TS21: germ cell of testis Absent GUDMAP:9664
TS21: developing vasculature of testis Absent GUDMAP:9664
TS21: mesonephric tubule of male Absent GUDMAP:9664 expression likely in the mesenchyme surrounding the tubules
TS21: paramesonephric duct of male, mesonephric portion Ambiguous GUDMAP:9664 structure mostly likely to be negative but uncertain due to the strong expression in the mesenchyme
TS21: interstitium of the testis Ambiguous GUDMAP:9664 expression possible but only apparent on the right testis
TS21: testis Ambiguous GUDMAP:9664

Specimen GUDMAP:9665: embryonic day 13.5 (more )
Note: Labeling was visualized using NBT/BCIP.
Structure Level Pattern Image Note
TS21: female reproductive system Present Regionally restricted GUDMAP:9665
TS21: mesonephros of female Present Regionally restricted GUDMAP:9665
TS21: mesonephric mesenchyme of female Present GUDMAP:9665
TS21: nephric duct of female, rest of Present GUDMAP:9665
TS21: mesenchyme of nephric duct of female Present GUDMAP:9665
TS21: paramesonephric duct of female, rest of Present GUDMAP:9665
TS21: mesenchyme of rest of paramesonephric duct of female Present GUDMAP:9665
TS21: ovary Present Regionally restricted GUDMAP:9665
TS21: mesenchymal stroma of ovary Present GUDMAP:9665
TS21: nephric duct of female, mesonephric portion Present GUDMAP:9665 strong expression in the mesenchyme surrounding the duct
TS21: mesonephric tubule of female Ambiguous GUDMAP:9665 expression possible
TS21: paramesonephric duct of female, mesonephric portion Ambiguous GUDMAP:9665 expression possible in mesenchyme surrounding epithelium of duct
TS21: epithelium of rest of paramesonephric duct of female Ambiguous GUDMAP:9665 expression most likely to be negative
TS21: epithelium of nephric duct of female Ambiguous GUDMAP:9665 expression mostly likely to be negative

Specimen GUDMAP:9666: embryonic day 13.5 (more )
Note: Labeling was visualized using NBT/BCIP. Male and female were examined; only the female is shown. Annotators reported the male expression pattern in the metanephros is identical to female.
Structure Level Pattern Image Note
TS21: urinary system Present Regionally restricted GUDMAP:9666
TS21: metanephros Present Regionally restricted GUDMAP:9666
TS21: nephrogenic interstitium Present GUDMAP:9666
TS21: renal interstitium Present GUDMAP:9666
TS21: cap mesenchyme Absent GUDMAP:9666
TS21: ureteric tip Absent GUDMAP:9666
TS21: ureter Ambiguous GUDMAP:9666 expression possible
TS21: urothelium of ureter Ambiguous GUDMAP:9666 expression possible
TS21: ureteral mesenchyme Ambiguous GUDMAP:9666 expression possible

Specimen GUDMAP:9667: embryonic day 13.5 (more )
Note: Labeling was visualized using NBT/BCIP.
Structure Level Pattern Image Note
TS21: urogenital sinus Present Regionally restricted GUDMAP:9667
TS21: primitive bladder Absent GUDMAP:9667
TS21: ureter Ambiguous GUDMAP:9667 expression possible
TS21: urothelium of ureter Ambiguous GUDMAP:9667 expression possible
TS21: ureteral mesenchyme Ambiguous GUDMAP:9667 expression possible

Specimen GUDMAP:10760: embryonic day 15.5 (more )
Note: Labeling was visualized using BM purple. The low level expression control (Shh) probe's color developed for 140 hrs, but no expression was detected. Thus, it is possible that very weak levels of gene expression were not detectable in this experiment.
Structure Level Pattern Image Note
TS23: urinary system Present GUDMAP:10760
TS23: metanephros Present Regionally restricted GUDMAP:10760
TS23: nephrogenic zone Present GUDMAP:10760
TS23: nephrogenic interstitium Present GUDMAP:10760
TS23: cap mesenchyme Present GUDMAP:10760
TS23: pretubular aggregate Present GUDMAP:10760
TS23: renal cortex Present Regionally restricted GUDMAP:10760
TS23: renal cortical interstitium Present Regionally restricted GUDMAP:10760
TS23: renal medulla Present Regionally restricted GUDMAP:10760
TS23: renal medullary interstitium Present GUDMAP:10760
TS23: ureter Present Regionally restricted GUDMAP:10760
TS23: ureteral mesenchyme outer layer Present GUDMAP:10760
TS23: ureteric tip Absent GUDMAP:10760
TS23: ureteric tree terminal branch excluding tip itself Absent GUDMAP:10760
TS23: renal vesicle Absent GUDMAP:10760
TS23: comma-shaped body Absent GUDMAP:10760
TS23: s-shaped body Absent GUDMAP:10760
TS23: capillary loop renal corpuscle Absent GUDMAP:10760
TS23: maturing renal corpuscle Absent GUDMAP:10760
TS23: cortical renal tubule Absent GUDMAP:10760
TS23: cortical collecting duct Absent GUDMAP:10760
TS23: renal cortical vasculature Absent GUDMAP:10760
TS23: immature loop of Henle Absent GUDMAP:10760
TS23: renal medullary vasculature Absent GUDMAP:10760
TS23: medullary collecting duct Absent GUDMAP:10760
TS23: pelvic urothelial lining Absent GUDMAP:10760
TS23: urothelium of ureter Absent GUDMAP:10760
TS23: ureteral vasculature Absent GUDMAP:10760
TS23: renal pelvis Ambiguous GUDMAP:10760
TS23: pelvic smooth muscle Ambiguous GUDMAP:10760 uncertain weak expression
TS23: perihilar interstitium Ambiguous GUDMAP:10760 uncertain weak expression
TS23: ureteral mesenchyme Ambiguous GUDMAP:10760 uncertain weak expression

Specimen GUDMAP:10761: embryonic day 15.5 (more )
Note: Labeling was visualized using BM purple. The low level expression control (Shh) probe's color developed for 140 hrs, but no expression was detected. Thus, it is possible that very weak levels of gene expression were not detectable in this experiment.
Structure Level Pattern Image Note
TS23: ureter Present GUDMAP:10761
TS23: ureteral mesenchyme outer layer Present GUDMAP:10761
TS23: urothelium of ureter Absent GUDMAP:10761
TS23: ureteral vasculature Absent GUDMAP:10761
TS23: ureteral mesenchyme Ambiguous GUDMAP:10761 uncertain weak expression

Specimen GUDMAP:10762: postnatal adult (more )
Note: This animal was sexually mature. Visualized using BM purple. The low level expression control (Shh) probe's color developed for 140 hrs, but no expression was detected. Thus, it is possible that very weak levels of gene expression were not detected.
Structure Level Pattern Image Note
TS28: metanephros Absent GUDMAP:10762


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last database update
05/14/2024
MGI 6.23
The Jackson Laboratory