About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:171409 GUDMAP Consortium, GUDMAP: the GenitoUrinary Development Molecular Anatomy Project. www.gudmap.org. 2004;
Assay type: RNA in situ
MGI Accession ID: MGI:5538830
Gene symbol: Spp1
Gene name: secreted phosphoprotein 1
Probe: MGC clone 6613
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen GUDMAP:10914: postnatal adult (more )
Note: This animal was sexually mature. Labeling was visualized using BM purple.
Structure Level Pattern Image Note
TS28: urinary system Present GUDMAP:10914
TS28: metanephros Present Regionally restricted GUDMAP:10914
TS28: renal cortex Present Regionally restricted GUDMAP:10914
TS28: cortical renal tubule Present Regionally restricted GUDMAP:10914
TS28: renal distal tubule Present Regionally restricted GUDMAP:10914
TS28: distal straight tubule of renal cortex Present GUDMAP:10914
TS28: postmacula segment of distal straight tubule Present GUDMAP:10914
TS28: premacula segment of distal straight tubule of renal cortex Present GUDMAP:10914
TS28: distal convoluted tubule Present Regionally restricted GUDMAP:10914 expression likely restricted to a subset of the distal convoluted tubule adjacent to the distal straight tubule
TS28: renal medulla Present Regionally restricted GUDMAP:10914
TS28: outer renal medulla Present Regionally restricted GUDMAP:10914
TS28: outer stripe of outer medulla Present Regionally restricted GUDMAP:10914
TS28: loop of Henle of outer stripe of outer medulla Present Regionally restricted GUDMAP:10914
TS28: distal straight tubule of outer stripe of outer medulla Present GUDMAP:10914
TS28: inner stripe of outer medulla Present Regionally restricted GUDMAP:10914
TS28: loop of Henle of inner stripe of outer medulla Present Regionally restricted GUDMAP:10914
TS28: premacula segment of distal straight tubule of inner stripe of inner medulla Present GUDMAP:10914
TS28: inner renal medulla Present Regionally restricted GUDMAP:10914
TS28: loop of Henle, inner medullary portion Present Regionally restricted GUDMAP:10914
TS28: thin ascending limb of inner medulla Present GUDMAP:10914
TS28: thin descending limb of inner stripe of outer medulla Present Regionally restricted GUDMAP:10914 distal expression; expression likely absent from a subset of thin descending limb adjacent to proximal tubule
TS28: thin descending limb of inner medulla Present Regionally restricted GUDMAP:10914 distal expression; expression likely absent from a subset of thin descending limb adjacent to the proximal tubule
TS28: cortical collecting duct Present Regionally restricted GUDMAP:10914 moderate Spp1 expression in a very small number of collecting duct tubules in the cortex. These tubules co-expressed the collecting duct marker protein, Aqp2. These collecting ducts represented a very-small subset of mature cortical nephrons of the adult kidney.
TS28: renal pelvis Present GUDMAP:10914
TS28: pelvic urothelial lining Present GUDMAP:10914
TS28: mature renal corpuscle Absent GUDMAP:10914
TS28: renal proximal tubule Absent GUDMAP:10914
TS28: renal connecting tubule Absent GUDMAP:10914
TS28: renal cortical interstitium Absent GUDMAP:10914
TS28: renal cortical vasculature Absent GUDMAP:10914
TS28: proximal straight tubule of outer stripe of outer medulla Absent GUDMAP:10914
TS28: outer medullary collecting duct Absent GUDMAP:10914
TS28: outer medullary interstitium Absent GUDMAP:10914
TS28: inner medullary collecting duct Absent GUDMAP:10914
TS28: inner medullary interstitium Absent GUDMAP:10914
TS28: renal medullary vasculature Absent GUDMAP:10914
Specimen GUDMAP:10915: postnatal adult (more )
Note: This animal was sexually mature. This assay's probe was visualized using BM purple; it is blue. The section was also subjected to immunohistochemistry with an anti-Aqp1 antibody; that labeling is orange.
Structure Level Pattern Image Note
TS28: urinary system Present GUDMAP:10915
TS28: metanephros Present Regionally restricted GUDMAP:10915
TS28: renal cortex Present Regionally restricted GUDMAP:10915
TS28: cortical renal tubule Present Regionally restricted GUDMAP:10915
TS28: renal distal tubule Present Regionally restricted GUDMAP:10915
TS28: distal straight tubule of renal cortex Present GUDMAP:10915
TS28: postmacula segment of distal straight tubule Present GUDMAP:10915
TS28: premacula segment of distal straight tubule of renal cortex Present GUDMAP:10915
TS28: distal convoluted tubule Present Regionally restricted GUDMAP:10915 expression likely restricted to a subset of the distal convoluted tubule adjacent to the distal straight tubule
TS28: renal medulla Present Regionally restricted GUDMAP:10915
TS28: outer renal medulla Present Regionally restricted GUDMAP:10915
TS28: outer stripe of outer medulla Present Regionally restricted GUDMAP:10915
TS28: loop of Henle of outer stripe of outer medulla Present Regionally restricted GUDMAP:10915
TS28: distal straight tubule of outer stripe of outer medulla Present GUDMAP:10915
TS28: inner stripe of outer medulla Present Regionally restricted GUDMAP:10915
TS28: loop of Henle of inner stripe of outer medulla Present Regionally restricted GUDMAP:10915
TS28: premacula segment of distal straight tubule of inner stripe of inner medulla Present GUDMAP:10915
TS28: inner renal medulla Present Regionally restricted GUDMAP:10915
TS28: loop of Henle, inner medullary portion Present Regionally restricted GUDMAP:10915
TS28: thin ascending limb of inner medulla Present GUDMAP:10915
TS28: thin descending limb of inner stripe of outer medulla Present Regionally restricted GUDMAP:10915 distal expression; expression likely absent from a subset of thin descending limb adjacent to proximal tubule
TS28: thin descending limb of inner medulla Present Regionally restricted GUDMAP:10915 distal expression; expression likely absent from a subset of thin descending limb adjacent to the proximal tubule
TS28: cortical collecting duct Present Regionally restricted GUDMAP:10915 moderate Spp1 expression in a very small number of collecting duct tubules in the cortex. These tubules co-expressed the collecting duct marker protein, Aqp2. These collecting ducts represented a very-small subset of mature cortical nephrons of the adult kidney.
TS28: renal pelvis Present GUDMAP:10915
TS28: pelvic urothelial lining Present GUDMAP:10915
TS28: mature renal corpuscle Absent GUDMAP:10915
TS28: renal proximal tubule Absent GUDMAP:10915
TS28: renal connecting tubule Absent GUDMAP:10915
TS28: renal cortical interstitium Absent GUDMAP:10915
TS28: renal cortical vasculature Absent GUDMAP:10915
TS28: proximal straight tubule of outer stripe of outer medulla Absent GUDMAP:10915
TS28: outer medullary collecting duct Absent GUDMAP:10915
TS28: outer medullary interstitium Absent GUDMAP:10915
TS28: inner medullary collecting duct Absent GUDMAP:10915
TS28: inner medullary interstitium Absent GUDMAP:10915
TS28: renal medullary vasculature Absent GUDMAP:10915
Specimen GUDMAP:10916: embryonic day 15.5 (more )
Note: This assay's probe was visualized using BM purple; it is blue. The section was also subjected to immunohistochemistry with an anti-Aqp1 antibody; that labeling is orange.
Structure Level Pattern Image Note
TS23: urinary system Present GUDMAP:10916
TS23: metanephros Present Regionally restricted GUDMAP:10916
TS23: renal cortex Present Regionally restricted GUDMAP:10916
TS23: cortical renal tubule Present Regionally restricted GUDMAP:10916
TS23: cortical early proximal tubule Present Regionally restricted GUDMAP:10916 strong Spp1 mRNA expression in a small subset of early proximal tubules of maturing nephron stage IV only
TS23: cortical early distal tubule Present GUDMAP:10916
TS23: renal medulla Present Regionally restricted GUDMAP:10916
TS23: immature loop of Henle Present Regionally restricted GUDMAP:10916 expression in a subset of immature loop of Henle some with overlapping expression with Aqp1, some not overlapping with Aqp1 and some loop of Henle absent for both Aqp1 and Spp1.
TS23: medullary collecting duct Present Regionally restricted GUDMAP:10916 small number of nephrons expressed Spp1 in the collecting duct. We observed very-weak expression in single cells of some collecting duct tubules.
TS23: renal pelvis Present Regionally restricted GUDMAP:10916
TS23: pelvic urothelial lining Present GUDMAP:10916
TS23: renal capsule Absent GUDMAP:10916
TS23: nephrogenic zone Absent GUDMAP:10916
TS23: renal vesicle Absent GUDMAP:10916
TS23: comma-shaped body Absent GUDMAP:10916
TS23: s-shaped body Absent GUDMAP:10916
TS23: capillary loop renal corpuscle Absent GUDMAP:10916
TS23: maturing renal corpuscle Absent GUDMAP:10916
TS23: anlage of loop of Henle of cortical renal tubule Absent GUDMAP:10916
TS23: renal connecting tubule Absent GUDMAP:10916
TS23: cortical collecting duct Absent GUDMAP:10916
TS23: renal cortical interstitium Absent GUDMAP:10916
TS23: renal cortical vasculature Absent GUDMAP:10916
TS23: renal medullary interstitium Absent GUDMAP:10916
TS23: renal medullary vasculature Absent GUDMAP:10916
TS23: pelvic smooth muscle Absent GUDMAP:10916
TS23: perihilar interstitium Absent GUDMAP:10916
TS23: ureter Absent GUDMAP:10916
TS23: cortical renal tubule of maturing nephron group Present Regionally restricted GUDMAP:10916
TS23: early proximal tubule of maturing nephron Present Regionally restricted GUDMAP:10916
TS23: early distal tubule of maturing nephron Present GUDMAP:10916
TS23: cortical renal tubule of capillary loop nephron group Absent GUDMAP:10916
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory