Probe:	Zac1 cDNA4
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		1A

Structure	Level	Pattern	Image	Note
TS15: cardiac muscle tissue	Present		1B

Structure	Level	Pattern	Image	Note
TS19: atrium cardiac muscle	Very strong		1G , 1F
TS19: ventricle cardiac muscle	Very strong		1G , 1F
TS19: blood vessel	Present	Regionally restricted	1F , 1I	Expression is along the wall of umbilical vessel and intrahepatic vessel.
TS19: liver	Present	Regionally restricted	1I , 1F	Expression is at the surface, weak in the parenchyma.
TS19: vertebral pre-cartilage condensation	Present		1J , 1F	Expression is at the site of mesenchymal condensation, which later becomes vertebral bodies.
TS19: endocardial cushion	Absent		1G , 1F

Structure	Level	Pattern	Image	Note
TS19: neural tube	Very strong	Non-Uniform	1K	Expression is stronger adjacent to the lumen of the neural tube.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present		2A
TS21: ventricle cardiac muscle	Present	Regionally restricted	2B , 2A	Expression is in the trabeculated ventricular myocardium.
TS21: pulmonary artery	Present	Regionally restricted	2B , 2A	Expression is in the wall with increased expression in the medial layers.
TS21: metanephros	Present	Regionally restricted	2E , 2A	Expression is in the capsule.
TS21: skeletal muscle	Strong	Regionally restricted	2A , 2E	Expression is in paravertebral skeletal muscle.
TS21: vertebral cartilage condensation	Strong	Regionally restricted	2A , 2E	Expression is at the site of chondrogenesis.
TS21: gut	Present	Regionally restricted	2A , 2F	Expression is around the adventitia of the gut.
TS21: pancreas	Present		2F , 2A
TS21: hindbrain	Present	Regionally restricted	2G , 2A	Expression is at the luminal surface.
TS21: choroid plexus	Present		2A , 2G
TS21: hyoid cartilage primordium	Strong		2A , 2H
TS21: tongue muscle	Strong		2A , 2H
TS21: lung	Strong	Regionally restricted	2A , 2I	Expression is in the serous surface of the lung.
TS21: liver	Strong	Regionally restricted	2A , 2I	Expression is in the capsule of the liver.

Structure	Level	Pattern	Image	Note
TS21: pulmonary artery	Strong	Regionally restricted	2C	Increased expression in the medial layers.

Structure	Level	Pattern	Image	Note
TS21: stomach	Present	Regionally restricted	2J

Structure	Level	Pattern	Image	Note
TS23: vertebra	Present		3A
TS23: sternum	Strong		3B , 3A
TS23: ventricle cardiac muscle	Weak		3B , 3A
TS23: aorta	Absent		3C , 3A
TS23: visceral pleura	Weak		3D , 3A
TS23: lung	Absent		3A , 3D
TS23: choroid plexus	Absent		3A , 3E

Structure	Level	Pattern	Image	Note
TS27: femur	Present	Regionally restricted	4A	Expression is in the perichondrium and the periarticular chondrocytes of the epiphysial cartilage, and in the layer of prehypertrophic chondrocytes, absent in hypertrophic chondrocytes.

Structure	Level	Pattern	Image	Note
TS27: femur	Present	Regionally restricted	4B	Expression is in periarticular chondrocytes in the epiphysial cartilage and the periosteum of the metaphysis and diaphysis.