Probe:	Tgfb2 probe6
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: cardiac muscle tissue	Strong	Regionally restricted	1C	Expression is in the dorsal myocardium.
TS15: arterial system	Weak	Regionally restricted	1C	Expression is in the walls of the great arteries.
TS15: dorsal aorta	Present	Regionally restricted	1C	Expression is at the medial side.
TS15: dorsal mesocardium	Present		1C
TS15: embryo endoderm	Present		1C
TS15: heart	Present	Regionally restricted	1C	Expression is in the proepicardial organ, including the epicardial cells that start to cover the ventricle.
TS15: common atrial chamber cardiac muscle	Weak		1C	Stronger expression in the ventricle wall of the left atrium.
TS15: primitive ventricle cardiac muscle	Weak		1C

Structure	Level	Pattern	Image	Note
TS15: atrioventricular canal	Strong	Regionally restricted	1F	Expression is in the endocardium.
TS15: cardiac muscle tissue	Strong	Regionally restricted	1F	Expression is in the myocradium underlying the cushions, the remainder is weakly positive (except future left atrium).
TS15: dorsal mesocardium	Present		1F
TS15: embryo endoderm	Present		1F
TS15: outflow tract	Present		1F
TS15: endocardial lining	Present	Regionally restricted	1F	Expression is in the endocardium and subendocardial cells in the atrio-ventricular segment.

Structure	Level	Pattern	Image	Note
TS15: outflow tract	Present		1I

Structure	Level	Pattern	Image	Note
TS17: heart	Present	Regionally restricted		Expression is in the developing epicardium.
TS17: endocardial cushion	Present	Single cells

Structure	Level	Pattern	Image	Note
TS19: blood vessel	Present	Regionally restricted	2C	Expression is in the media and adventitia of the vascular system.
TS19: atrioventricular canal	Present	Regionally restricted	2C	Expression is in the mesenchyme of the endocardial cushions of the atrioventricular canal and outflow tract.
TS19: endocardial cushion	Present	Regionally restricted	2C	Expression is in the mesenchyme of the endocardial cushions of the atrioventricular canal and outflow tract.
TS19: outflow tract	Present	Regionally restricted	2C	Expression is in the mesenchyme of the endocardial cushions of the atrioventricular canal and outflow tract.
TS19: cardiac muscle tissue	Weak	Regionally restricted	2C	The majority is weak, strong in areas of atrioventricular canal and outflow tract, moderate in atrial septum, absent in the compact myocardium which underlies the epicardium.
TS19: endocardial lining	Absent

Structure	Level	Pattern	Image	Note
TS19: blood vessel	Present	Regionally restricted	2F	Expression is in the media and adventitia of the vascular system.
TS19: atrioventricular canal	Present	Regionally restricted	2F	Expression is in the mesenchyme of the endocardial cushions of the atrioventricular canal and outflow tract.
TS19: endocardial cushion	Present	Regionally restricted	2F	Expression is in the mesenchyme of the endocardial cushions of the atrioventricular canal and outflow tract.
TS19: outflow tract	Present	Regionally restricted	2F	Expression is in the mesenchyme of the endocardial cushions of the atrioventricular canal and outflow tract.
TS19: endocardial lining	Absent		2F

Structure	Level	Pattern	Image	Note
TS19: cardiac muscle tissue	Weak	Regionally restricted	2I	The majority is weak, strong in areas of atrioventricular canal and outflow tract, moderate in atrial septum, absent in the compact myocardium which underlies the epicardium.
TS19: visceral pericardium	Present	Regionally restricted	2I	Expression is in the epicardium.

Structure	Level	Pattern	Image	Note
TS19: cardiac muscle tissue	Weak	Regionally restricted	2L	The majority is weak, strong in areas of atrioventricular canal and outflow tract, moderate in atrial septum, absent in the compact myocardium which underlies the epicardium.
TS19: visceral pericardium	Present	Regionally restricted	2L	Expression is in the epicardium.

Structure	Level	Pattern	Image	Note
TS20: heart	Present	Regionally restricted	3C	Expression is in the condensed mesenchyme, less in cushion mesenchyme, weak in the epicardium.
TS20: cardiac muscle tissue	Present	Regionally restricted	3C	Expression is in the compact myocardium underlying the epicardium.
TS20: atrioventricular canal	Present		3C

Structure	Level	Pattern	Image	Note
TS20: heart	Present	Regionally restricted	3G	Expression is in the condensed mesenchyme, less in cushion mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: visceral pericardium	Weak	Regionally restricted	3K	Expression is in the epicardium.

Structure	Level	Pattern	Image	Note
TS20: visceral pericardium	Weak	Regionally restricted	3O	Expression is in the epicardium.
TS20: cardiac muscle tissue	Present	Regionally restricted	3O	Expression is in the compact myocardium underlying the epicardium.

Structure	Level	Pattern	Image	Note
TS21: cardiac muscle tissue	Present	Regionally restricted	4C	Expression is in the myocardium underlying the atrioventricular cushions.
TS21: visceral pericardium	Weak	Regionally restricted	4C	Expression is in the epicardium and is stronger over the atria, ventricles and atrioventricular sulcus.

Structure	Level	Pattern	Image	Note
TS21: ascending aorta	Present	Regionally restricted	4G	Expression is in the media and adventitia of the vessel wall.
TS21: visceral pericardium	Weak	Regionally restricted	4G	Expression is in the epicardium and is stronger over the atria, ventricles and atrioventricular sulcus.
TS21: outflow tract	Present	Regionally restricted	4G	Expression is in the cushion mesenchyme and in the area in which the myocardialization of the outflow tarct septum occurs.

Structure	Level	Pattern	Image	Note
TS21: ascending aorta	Present	Regionally restricted	4K	Expression is in the media and adventitia of the vessel wall.
TS21: outflow tract	Present	Regionally restricted	4K	Expression is in the outflow tract cushions and is weaker at the distal valvar end.
TS21: interatrial septum	Weak		4K
TS21: heart atrium	Weak	Regionally restricted	4K	Absent in the lateral wall, weak in the septum.

Structure	Level	Pattern	Image	Note
TS22: visceral pericardium	Present	Regionally restricted	5C	Expression is in the epicardium over the atrioventricular sulcus and covering the atria.
TS22: atrioventricular canal	Present	Regionally restricted	5C	Expression is in the mesenchyme.
TS22: outflow tract	Present	Regionally restricted	5C	Expression is in the mesenchyme.
TS22: heart valve	Strong	Regionally restricted	5C	Strongest staining at the free margins of the valve leaflets.
TS22: impulse conducting system	Present	Regionally restricted	5C	Expression is in the location of the developing His-Purkinje system.

Structure	Level	Pattern	Image	Note
TS22: dorsal aorta	Present	Regionally restricted	5G	Expression is in the media and adventitia of the vessel wall.
TS22: atrioventricular canal	Present	Regionally restricted	5G	Expression is in the mesenchyme.
TS22: outflow tract	Present	Regionally restricted	5G	Expression is in the mesenchyme.
TS22: heart	Present	Regionally restricted	5G	Expression is in the residual mesenchymal tissue localised immediately below aortic valve level.
TS22: heart valve	Strong	Regionally restricted	5G	Strongest staining at the free margins of the valve leaflets.

Structure	Level	Pattern	Image	Note
TS23: atrioventricular canal	Present	Regionally restricted	6C	Expression is in the mesenchyme.
TS23: cardiac valve leaflet	Strong	Regionally restricted	6C	Strongest staining at the free margins of the valve leaflets.
TS23: impulse conducting system	Present	Regionally restricted	6C	Expression is in the location of the developing His-Purkinje system.

Structure	Level	Pattern	Image	Note
TS23: descending aorta	Present	Regionally restricted	6G	Expression is in the media and adventitia of the vessel wall.
TS23: atrioventricular canal	Present	Regionally restricted	6G	Expression is in the mesenchyme.
TS23: heart	Present	Regionally restricted	6G	Expression is in the residual mesenchymal tissue localised immediately below aortic valve level.
TS23: heart valve	Present	Regionally restricted	6G	Expression is in the proximal part of the semilunar valves.
TS23: cardiac valve leaflet	Strong	Regionally restricted	6G	Strongest staining at the free margins of the valve leaflets.

Structure	Level	Pattern	Image	Note
TS23: descending aorta	Present	Regionally restricted	6K	Expression is in the media and adventitia of the vessel wall.

Structure	Level	Pattern	Image	Note
TS23: visceral pericardium	Present	Regionally restricted	6O	Weak expression in the atrioventricular sulcus, strong in the epicardium covering the atria.

Structure	Level	Pattern	Image	Note
TS23: visceral pericardium	Present	Regionally restricted	6S	Weak expression in the atrioventricular sulcus, strong in the epicardium covering the atria.