Probe:	Gata2 probe3
Probe preparation:	Antisense RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: otocyst epithelium	Weak	Regionally restricted	1A	Expressed in the dorsolateral epithelium wall.

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present	Regionally restricted	1C	Expressed in separate dorsal (strong) and ventral (weaker) domains.
TS17: otocyst mesenchyme	Absent		1C

Structure	Level	Pattern	Image	Note
TS19: vestibular component epithelium	Present	Regionally restricted	1E	Highest expressionn was in the dorsal otic epithelium in the areas that will give rise to the semicircular ducts.
TS19: inner ear mesenchyme	Present	Regionally restricted	1E	Weaker expression in in the mesenchyme surrounding the future superior semicircular duct epithelium.
TS19: saccule	Present	Regionally restricted	1E	Expressed in the tip of the ventrally extending cochlear duct.
TS19: endolymphatic appendage	Absent		1E
TS19: saccule epithelium	Absent		1E
TS19: saccule mesenchyme	Absent		1E

Structure	Level	Pattern	Image	Note
TS20: utricle	Present	Regionally restricted	1G	Strongest expression was in the newly formed semicircular ducts, in the dorsal utricle and adjacent mesenchymal cells.
TS20: utricle mesenchyme	Present	Regionally restricted	1G	Strongest expression was in the mesenchymal cells adiacent to the dorsal utricle.
TS20: cochlear duct	Present	Regionally restricted	1G	Expressed in the lateral wall.
TS20: saccule epithelium	Absent		1G
TS20: saccule mesenchyme	Absent		1G

Structure	Level	Pattern	Image	Note
TS22: vestibular component epithelium	Strong	Regionally restricted	2A	Expressed in the vestibular epithelium except the saccule. Expression is strictly restricted to the nonsensory domains.
TS22: saccule epithelium	Absent		2A
TS22: inner ear vestibular component	Strong	Regionally restricted	2A	Expressed in the vestibular mesenchyme except the saccule.
TS22: cochlear duct	Present	Regionally restricted	2A	Expressed on one side.
TS22: semicircular canal epithelium	Present	Regionally restricted	2A	No expression in the crista of the semicircular ducts.
TS22: utricle epithelium	Present	Regionally restricted	2A	No expression in the macula of the utricle.
TS22: vestibular ganglion	Present	Uniform	2A
TS22: cochlear ganglion	Present	Regionally restricted	2A	Expressed in only a small subset of cells located on the outer edges.

Structure	Level	Pattern	Image	Note
TS22: cochlear duct	Present	Regionally restricted	2C	Expressed on one side.
TS22: cochlear ganglion	Present	Regionally restricted	2C	Expressed in only a small subset of cells located on the outer edges.

Structure	Level	Pattern	Image	Note
TS26: utricle epithelium	Present	Regionally restricted	2E	No expression in the crista of the semicircular ducts and the macula of the utricle.
TS26: vestibular component epithelium	Present	Regionally restricted	2E	Expression is strictly restricted to the nonsensory domains.

Structure	Level	Pattern	Image	Note
TS26: cochlear duct	Present		2G
TS26: organ of Corti	Absent		2G

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present		4A
TS17: otocyst epithelium	Present		4A

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Weak		4B
TS17: hindbrain	Present		4B	Same as in the wild-type.

Structure	Level	Pattern	Image	Note
TS19: vestibular component epithelium	Present		4C

Structure	Level	Pattern	Image	Note
TS19: vestibular component epithelium	Present	Regionally restricted	4D	Reduced expression in the most dorsal and ventral tips of the mutant otic epithelium, corresponding most likely to the remnants of vestibular and cochlear compartments.

Structure	Level	Pattern	Image	Note
TS17: endolymphatic appendage	Absent

Structure	Level	Pattern	Image	Note
TS20: endolymphatic appendage	Absent

Structure	Level	Pattern	Image	Note
TS26: vestibulo-cochlear ganglion	Present

Structure	Level	Pattern	Image	Note
TS15: otocyst	Absent