Gene Expression Data
Assay Details
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Assay
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Reference:
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J:82215
Liu W, et al., Bone morphogenetic protein 4 (BMP4): A regulator of capsule chondrogenesis in the developing mouse inner ear. Dev Dyn. 2003 Mar;226(3):427-38
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5529119
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Gene symbol:
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Bmp4
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Gene name:
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bone morphogenetic protein 4
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Antibody:
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Anti-Bmp4
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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The fixative was methacarn.
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Results
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Specimen
1A:
embryonic day 10.0
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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involves: C57BL/6J * CBA/J
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Age:
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embryonic day 10.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
1B:
embryonic day 11.5
(more )
Specimen
1B:
(close )
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Genetic Background:
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involves: C57BL/6J * CBA/J
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: inner ear mesenchyme |
Present |
Regionally restricted |
1B
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Expression was detected in the condensing periotic mesenchyme that is dorsal and lateral to the horizontal semicircular duct and to the mesenchyme that is lateral to developing vestibular structures.
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Specimen
1C:
embryonic day 12.5
(more )
Specimen
1C:
(close )
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Genetic Background:
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involves: C57BL/6J * CBA/J
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: otic capsule |
Present |
Regionally restricted |
1C
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Expression was detected in condensing mesenchyme of the forming cartilaginous otic capsule.
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| TS20: inner ear mesenchyme |
Present |
Regionally restricted |
1C
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Expression was detected in the perilymphatic space mesenchyme adjacent to the utricle.
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| TS20: vestibular ganglion |
Present |
Regionally restricted |
1C
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Dense staining was detected in nerve fibers emerging from the vestibular ganglion.
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Specimen
1D:
embryonic day 12.5
(more )
Specimen
1D:
(close )
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Genetic Background:
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involves: C57BL/6J * CBA/J
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: otic capsule |
Present |
Regionally restricted |
1D
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Expression was detected in the condensing mesenchyme of the developing otic capsule.
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| TS20: inner ear mesenchyme |
Present |
Regionally restricted |
1D
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Expression was detected in the perilymphatic space mesenchyme adjacent to the developing cochlear duct.
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| TS20: cochlear ganglion |
Present |
Regionally restricted |
1D
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Expression was detected in fibers emerging from the acoustic ganglion toward the cochlear duct.
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Specimen
1E:
embryonic day 13.5
(more )
Specimen
1E:
(close )
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Genetic Background:
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involves: C57BL/6J * CBA/J
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: inner ear mesenchyme |
Present |
Regionally restricted |
1E
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Expression was detected in the perilymphatic space mesenchyme.
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| TS21: cochlear ganglion |
Present |
Regionally restricted |
1E
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Expression was detected in the emerging nerve fibers of the acoustic and vestibular ganglia.
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| TS21: vestibular ganglion |
Present |
Regionally restricted |
1E
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Expression was detected in the emerging nerve fibers of the acoustic and vestibular ganglia.
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| TS21: otic capsule |
Weak |
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1E
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Expression was detected in the chondrifying otic capsule.
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