Probe:	Gbx2 cDNA2
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS12: embryo	Present	Regionally restricted	2B	Expression is adjacent to the presumptive midbrain in the posterior part of the embryo.
TS12: midbrain-hindbrain junction	Present		2B

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present		2D
TS12: midbrain-hindbrain junction	Present		2D	There is a sharp anterior border at the midbrain/hindbrain boundary.
TS12: embryo	Present	Regionally restricted	2D	Expression is in the posterior region.
TS12: foregut diverticulum	Present	Regionally restricted	2D	Expression is in the foregut at the level of the first pharyngeal pocket.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present		2I
TS13: midbrain-hindbrain junction	Present	Regionally restricted	2I	There is a sharp anterior border at the midbrain/hindbrain boundary.
TS13: embryo	Present	Regionally restricted	2I	Expression is in the posterior region.
TS13: foregut diverticulum	Present	Regionally restricted	2I	Expression is in the foregut at the level of the first pharyngeal pocket.

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Present		3B
TS15: future spinal cord	Present		3B
TS15: midbrain-hindbrain junction	Present		3B
TS15: otocyst	Present		3B
TS15: rhombomere 1	Present		3B
TS15: rhombomere 2	Present		3B
TS15: rhombomere 3	Present		3B
TS15: rhombomere 4	Present		3B
TS15: rhombomere 7	Present		3B
TS15: rhombomere 8	Present		3B

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Present		4E
TS17: limb	Present		4E
TS17: otocyst	Present		4E

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present		4F	Expression is in the medial ganglionic eminence and the corpus striatum.
TS17: diencephalon	Present	Regionally restricted	4F	Expression is in the thalamus.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		4G	Expression is dorsal.

Structure	Level	Pattern	Image	Note
TS20: medial ganglionic eminence	Present		4H