Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:93087
Bajanca F, et al., Integrins in the mouse myotome: developmental changes and differences between the epaxial and hypaxial lineage. Dev Dyn. 2004 Oct;231(2):402-15
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:5527424
|
Gene symbol:
|
Itga4
|
Gene name:
|
integrin alpha 4
|
Probe:
|
alpha-4 probe1
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
|
Results
|
Specimen
not shown:
embryonic day 8.5-9.0
(more )
Specimen
not shown:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 8.5-9.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1F:
embryonic day 9.0
(more )
Note: Early E9.0 embryo.
Specimen
1F:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Early E9.0 embryo.
|
Specimen
1G:
embryonic day 9.0
(more )
Note: Early E9.0 embryo.
Specimen
1G:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Early E9.0 embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: myotome |
Present |
Regionally restricted |
1G
|
Expression was detected in the central region (on a medial to lateral axis) of rostral myotomes. The dorsomedial-most region of the myotome was negative for expression.
|
Specimen
1K:
embryonic day 9.5
(more )
Note: Late E9.5 embryo.
Specimen
1K:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Late E9.5 embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: somite |
Present |
Regionally restricted |
1K
|
Expression was detected in younger somites and is concentrated in the hypaxial region.
|
Specimen
1R:
embryonic day 10.5
(more )
Specimen
1R:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: dermomyotome |
Present |
Regionally restricted |
1R
|
Expression was detected in epaxial dermomyotomal lips.
|
Specimen
1T:
embryonic day 10.5
(more )
Specimen
1T:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: somite |
Present |
Regionally restricted |
1T
|
Expression was detected in younger somites and is concentrated in the hypaxial region.
|
TS17: myotome |
Present |
Regionally restricted |
1T
|
Expression was detected in interlimb level hypaxial myotomes and in intercalated myotome.
|
TS17: dermomyotome |
Present |
Regionally restricted |
1T
|
Rostrally, expression was detected in epaxial dermomyotomal lips.
|
|