Probe:	Grn probe
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS11: primary trophoblast giant cell	Strong		1A
TS28: placenta	Strong	Regionally restricted	1A	Expressed in decidualizing stromal cells.

Structure	Level	Pattern	Image	Note
TS13: labyrinthine zone	Strong		1B
TS28: decidua	Present		1B	Expression declines throughout the decidual placenta.
TS13: spongiotrophoblast	Absent		1B
TS13: trophoblast giant cell	Absent		1B
TS13: neural ectoderm	Present	Regionally restricted	1B	Expressed in the proliferating neuroepithelium.
TS13: embryo ectoderm	Present		1B
TS13: embryo mesenchyme	Present		1B

Structure	Level	Pattern	Image	Note
TS13: labyrinthine zone	Strong		1C
TS28: decidua	Present		1C	Expression declines throughout the decidual placenta.
TS13: spongiotrophoblast	Absent		1C
TS13: trophoblast giant cell	Absent		1C

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Regionally restricted	1E	Expressed in epiblast cells and migratory profiles.
TS11: embryo mesenchyme	Present		1E
TS11: extraembryonic mesenchyme	Present		1E
TS11: allantois mesoderm	Present		1E
TS11: chorion mesoderm	Present		1E
TS11: yolk sac mesoderm	Present		1E
TS11: embryo endoderm	Absent		1E
TS11: yolk sac endoderm	Absent		1E
TS11: primary trophoblast giant cell	Present	Regionally restricted	1E	Expressed in trophoblast giant cells at the embryo-maternal interface.

Structure	Level	Pattern	Image	Note
TS23: visceral pericardium	Present	Regionally restricted	1F	Expressed in the active blood vessel formation sites in the epicardium of the heart wall.
TS23: blood	Present		1F

Structure	Level	Pattern	Image	Note
TS21: brain pia mater	Present	Regionally restricted	1G	Expressed in blood vessels of the pia mater of meninges.

Structure	Level	Pattern	Image	Note
TS22: rib cartilage condensation	Strong	Regionally restricted	2A	No expression in the precursor perichondrial cells. Expressed in the more central hypertrophied chondrocytes.

Structure	Level	Pattern	Image	Note
TS24: liver	Present	Regionally restricted	2B	Expressed in the mononuclear blast-like cells.
TS24: epidermis	Present	Regionally restricted	2B	Expressed in the basal epidermal cells and at least one layer higher into the suprabasal layers of the fetal epidermis.
TS24: dermis	Present	Regionally restricted	2B	Expressed in the abdominal wall associated with developing skeletal muscle layers in the dermis. Expression is weaker than in the epidermis.

Structure	Level	Pattern	Image	Note
TS23: epidermis	Strong	Regionally restricted	2D	Expressed in the origin of the bud.
TS23: dermis	Weak	Regionally restricted	2D	Expressed in the mesenchyme. The appendage-associated hypertrophied mesenchyme was distinctly negative from surrounding tissues.

Structure	Level	Pattern	Image	Note
TS23: epidermis	Strong	Regionally restricted	2E	At a later phase, strong expression in the original epithelial bud site, but not in the distal epithelial ingrowth.
TS23: dermis	Weak	Regionally restricted	2E	Expressed in the mesenchyme. The in-growth associated hypertrophied mesenchyme was distinctly negative from surrounding tissues.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: testis mesenchyme		Present		2F
TS24: Sertoli cell	Sertoli cell	Present		2F
TS24: germ cell of testis		Absent		2F

Structure	Level	Pattern	Image	Note
TS24: renal tubule	Present	Regionally restricted	2G	Expressed in renotubular epithelia.
TS24: thoracic ganglion	Present		2G	Expressed in the aortic sympathetic ganglion.

Structure	Level	Pattern	Image	Note
TS24: dorsal root ganglion	Present		2H
TS24: spinal cord	Present		2H
TS24: spinal cord lateral wall	Present	Regionally restricted	2H	Expressed in the ventral horn.

Structure	Level	Pattern	Image	Note
TS22: forebrain	Strong	Regionally restricted	2I	Expressed in the neopallial cortex but not the intermediate or ventricular zones.
TS22: forebrain ventricular layer	Absent		2I
TS22: neocortex	Strong	Widespread	2I
TS22: cerebral cortex intermediate zone	Absent		2I
TS22: cerebral cortex ventricular layer	Absent		2I

Structure	Level	Pattern	Image	Note
TS24: hair follicle	Ambiguous	Regionally restricted		Absent or low in epithelial cells of the hair follicles.

Structure	Level	Pattern	Image	Note
TS24: liver vascular element	Absent			No expression in the epithelial cells.
TS24: lung	Absent
TS24: intestine epithelium	Weak