Gene Expression Data
Assay Details
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Assay
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Reference:
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J:84447
Cui XM, et al., TGF-beta3-dependent SMAD2 phosphorylation and inhibition of MEE proliferation during palatal fusion. Dev Dyn. 2003 Jul;227(3):387-94
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5527188
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Gene symbol:
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Smad3
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Gene name:
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SMAD family member 3
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Antibody:
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Anti-Smad3
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin
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Results
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Specimen
4A:
embryonic day 14.0
(more  )
Specimen
4A:
(close  )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 14.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: oral epithelium |
Present |
Regionally restricted |
4A
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Expressed in murine medial edge epithelium and oral epithelium prior to the opposing palatal shelves contacted in the midline.
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| TS22: palatal shelf epithelium |
Present |
Regionally restricted |
4A
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Expressed in murine medial edge epithelium and oral epithelium prior to the opposing palatal shelves contacted in the midline.
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Specimen
4B:
embryonic day 14.5
(more )
Specimen
4B:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: palatal shelf epithelium |
Present |
Regionally restricted |
4B
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Expression remained in the midline epithelial seam of palate as palatal fusion process.
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Specimen
4C:
embryonic day 15.0
(more )
Note: Late E15.
Specimen
4C:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 15.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Late E15.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: oral epithelium |
Present |
Regionally restricted |
4C
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After palate fused and mesenchyme confluence, the expression is not found in the midline except in a murine medial edge epithelium island but persists in the oral epithelium.
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| TS23: primary palate epithelium |
Present |
Regionally restricted |
4C
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After palate fused and mesenchyme confluence, the expression is not found in the midline except in a murine medial edge epithelium island but persists in the oral epithelium.
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