About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:90276 Ota MS, et al., Twist is required for patterning the cranial nerves and maintaining the viability of mesodermal cells. Dev Dyn. 2004 Jun;230(2):216-28
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5527082
Gene symbol: Nefm
Gene name: neurofilament, medium polypeptide
Antibody: anti-neurofilament clone 2H3
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes: The authors state that no differences in phenotype were seen between +/+ and +/- embryos and they were pooled as "normal" (+/+) for this study.
Results
Specimen 1J: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: acoustic ganglion Present 1J
TS18: facial ganglion Present 1J
TS18: glossopharyngeal ganglion Present 1J
TS18: inferior vagus ganglion Present 1J
TS18: superior vagus ganglion Present 1J
TS18: trigeminal ganglion Present 1J
Specimen 1K: embryonic day 11.0; Twist1tm1Bhr/Twist1tm1Bhr (more )
Structure Level Pattern Image Note
TS18: cranial ganglion Present 1K There are abnormal cranial ganglia and nerve patterns in this mutant.
Specimen 1L: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: acoustic ganglion Present 1L
TS18: facial ganglion Present 1L
TS18: trigeminal ganglion Present 1L
Specimen 1M: embryonic day 11.0; Twist1tm1Bhr/Twist1tm1Bhr (more )
Structure Level Pattern Image Note
TS18: cranial ganglion Present 1M There are abnormal cranial ganglia and nerve patterns in this mutant.
Specimen 1N: embryonic day 11.0; Twist1tm1Bhr/Twist1tm1Bhr (more )
Structure Level Pattern Image Note
TS18: cranial ganglion Present 1N There are abnormal cranial ganglia and nerve patterns in this mutant.
Specimen 1O: embryonic day 11.0; Twist1tm1Bhr/Twist1tm1Bhr (more )
Structure Level Pattern Image Note
TS18: cranial ganglion Present 1O There are abnormal cranial ganglia and nerve patterns in this mutant.
Specimen 1P: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: glossopharyngeal ganglion Present 1P
TS18: vagus ganglion Present 1P
Specimen 1Q: embryonic day 11.0; Twist1tm1Bhr/Twist1tm1Bhr (more )
Structure Level Pattern Image Note
TS18: cranial ganglion Present 1Q There are abnormal cranial ganglia and nerve patterns in this mutant.
Specimen 1R: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: spinal ganglion Present 1R
TS18: spinal nerve Present 1R
Specimen 1S: embryonic day 11.0; Twist1tm1Bhr/Twist1tm1Bhr (more )
Structure Level Pattern Image Note
TS18: spinal ganglion Present 1S Pattern is the same as in wild-ype embryos.
TS18: spinal nerve Present 1S Pattern is the same as in wild-ype embryos.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory