Summary | Transgene origin | Transgene description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Tg(Pdpn-icre)14Biat
Name:	transgene insertion 14, Biomodels Austria
MGI ID:	MGI:5526988
Synonyms:	Pdpn-Cre, transgene insertion 14, Thomas Ruelicke
Transgene:	Tg(Pdpn-icre)14Biat  Location: unknown
Alliance:	Tg(Pdpn-icre)14Biat page

Strain of Origin:

C57BL/6N

Transgene Type:    Transgenic (Recombinase) Mutation:    Insertion   Tg(Pdpn-icre)14Biat expression driven by 1 gene Transgene expression driven by: Organism Driver Gene Note Not Specified Pdpn   Mutation details: A BAC containing the podoplanin locus was modified, replacing exon 1 with a codon-optimized cre sequence and polyadenylation site. The cre sequence was integrated downstream of the start codon. Microinjection of the construct into pronuclei of zygotes produced 17 founders with 3 (406-408) positive for cre recombinase and the 5' ATG region by southern blot. These founders were designated 6, 12, and 14. When crossed to EYFP reporter mice, line 12 offspring did not have detectable cre expression, while line 6 offspring had some EYFP expression in the CD45+ve non-stromal cell compartment. Thus line 6 was excluded from further analysis. Offspring from line 14 had no cre activity in non-stromal cells and substantial activity in CD45-ve LN (lymph node) cells (in 15% in LEC and FRC fractions and very low expression in BECs or absent in the double-negative fractions.) (J:203412)

Activity:	Tissue activity of this recombinase allele
Driver:	Pdpn (species not specified) Summary of all recombinase alleles driven by Pdpn.

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:203412 Onder L, et al., A novel bacterial artificial chromosome-transgenic podoplanin-cre mouse targets lymphoid organ stromal cells in vivo. Front Immunol. 2011;2:50
All:	2 reference(s)