Probe:	S8 cDNA
Probe preparation:	Antisense labelled with radioactivity RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	2B	Expressed in mesoderm posterior from and lateral to the primitive streak.
TS28: decidua	Present	Regionally restricted	2B	Expressed in a characteristic pattern.
TS11: embryo ectoderm	Absent		2B
TS11: future brain	Absent		2B

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Present		3A right
TS15: mesenchyme derived from somatopleure	Present		3A right

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Present		3C
TS17: body wall	Present	Regionally restricted	3C	Expressed in the somatopleure.

Structure	Level	Pattern	Image	Note
TS22: heart valve	Present		6G	Expressed in the developing semilunar and atrioventricular valves.
TS22: interventricular septum muscular part	Present		6G
TS22: ductus arteriosus	Strong		6G

Structure	Level	Pattern	Image	Note
TS22: heart valve	Present		6H	Expressed in the developing semilunar and atrioventricular valves.
TS22: interventricular septum muscular part	Present		6H

Structure	Level	Pattern	Image	Note
TS22: heart valve	Present		6I	Expressed in the developing semilunar and atrioventricular valves.
TS22: interventricular septum muscular part	Present		6I
TS22: heart atrium	Strong	Regionally restricted	6I	Expressed in the posterior wall of the atrium, which is of sinus venosus origin.

Structure	Level	Pattern	Image	Note
TS25: heart valve	Present		7D	Expressed in the semilunar and atrioventricular valve areas.
TS25: heart mesentery	Present	Regionally restricted	7D	Expressed in the future fibrous heart skeleton including the fibrous trigone.
TS25: cardiac muscle tissue	Present	Regionally restricted	7D	Expressed in the myocardium which may be the developing chordae of the atrioventricular valves.

Structure	Level	Pattern	Image	Note
TS25: heart valve	Present		7E	Expressed in the semilunar and atrioventricular valve areas.
TS25: heart mesentery	Present	Regionally restricted	7E	Expressed in the future fibrous heart skeleton including the fibrous trigone.
TS25: cardiac muscle tissue	Present	Regionally restricted	7E	Expressed in the myocardium which may be the developing chordae of the atrioventricular valves.

Structure	Level	Pattern	Image	Note
TS25: heart valve	Present		7F	Expressed in the semilunar and atrioventricular valve areas.
TS25: heart mesentery	Present	Regionally restricted	7F	Expressed in the future fibrous heart skeleton including the fibrous trigone.
TS25: cardiac muscle tissue	Present	Regionally restricted	7F	Expressed in the myocardium which may be the developing chordae of the atrioventricular valves.

Structure	Level	Pattern	Image	Note
TS25: humerus	Absent		9C
TS25: ulna	Absent		9C
TS25: forelimb mesenchyme	Present	Regionally restricted	9C	Expressed in a thin layer of one or two cells around the chondrogenic centers, in or near the perichondrium.

Structure	Level	Pattern	Image	Note
TS25: palmar pad	Present		9D
TS25: hand mesenchyme	Present	Regionally restricted	9D	Expressed in tendons and near perichondrium.
TS25: forelimb digit 3 metacarpal	Absent		9D
TS25: forelimb digit 4 metacarpal	Absent		9D

Structure	Level	Pattern	Image	Note
TS9: embryo mesoderm	Present	Regionally restricted		Expressed in mesoderm posterior from and lateral to the primitive streak.
TS28: decidua	Present	Regionally restricted		Expressed in a characteristic pattern.

Structure	Level	Pattern	Image	Note
TS13: 1st branchial arch	Strong
TS13: yolk sac mesenchyme	Present
TS13: cardiac muscle tissue	Present	Diffuse

Structure	Level	Pattern	Image	Note
TS20: cardiac muscle tissue	Present	Diffuse

Structure	Level	Pattern	Image	Note
TS28: brain meninges	Present