Gene Expression Data
Assay Details
Assay
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Reference:
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J:151374
Teichert AM, et al., Endothelial nitric oxide synthase gene expression during murine embryogenesis: commencement of expression in the embryo occurs with the establishment of a unidirectional circulatory system. Circ Res. 2008 Jul 3;103(1):24-33
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5524970
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Gene symbol:
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Nos3
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Gene name:
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nitric oxide synthase 3, endothelial cell
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Probe:
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eNOS probe
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Assay notes:
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Probess were alkali hydrolyzed to a mean size of
70 nucleotides.
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Results
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Specimen
6A:
embryonic day 9.5
(more )
Specimen
6A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS15: blood vessel |
Present |
Regionally restricted |
6A
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Expression is in the endothelium of developing blood vessels, including the arteries of the aortic arch, intersomitic arteries, vessels of the limb bud, and vessels at the periphery of the neural tube and the brain.
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TS15: endocardial lining |
Present |
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6A
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Specimen
6B:
embryonic day 11.5
(more )
Specimen
6B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6C:
embryonic day 14.5
(more )
Specimen
6C:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS22: blood vessel |
Present |
Regionally restricted |
6C
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Expression is restricted to medium- to large-sized blood vessels and include vessels of the hindlimb, vessels of the stomach, intestine, lung, and in endothelial cells of the blood vessels surrounding the forebrain and hindbrain.
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Specimen
6D:
embryonic day 14.5
(more )
Specimen
6D:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
|
Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: blood vessel |
Present |
Regionally restricted |
6D
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Expression is restricted to medium- to large-sized blood vessels and include vessels of the hindlimb, vessels of the stomach, intestine, lung, and in endothelial cells of the blood vessels surrounding the forebrain and hindbrain.
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Specimen
not shown E17.5:
embryonic day 17.5
(more )
Specimen
not shown E17.5:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
not shown E11.5:
embryonic day 11.5
(more )
Specimen
not shown E11.5:
(close )
Genetic Background:
|
Not Specified
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Age:
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embryonic day 11.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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