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Gene Expression Data
Assay Details
Assay
Reference: J:69362 Koushik SV, et al., Targeted inactivation of the sodium-calcium exchanger (Ncx1) results in the lack of a heartbeat and abnormal myofibrillar organization. FASEB J. 2001 May;15(7):1209-11
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:5523261
Gene symbol: Slc8a1
Gene name: solute carrier family 8 (sodium/calcium exchanger), member 1
Reporter: lacZ
Direct Detection
Results
Specimen 2a: embryonic day 7.5; Slc8a1tm1Sjc/Slc8a1+ (more )
Structure Level Pattern Image Note
TS11: embryo Absent 2a
TS11: extraembryonic component Absent 2a

Specimen 2b/c: embryonic day 8.0; Slc8a1tm1Sjc/Slc8a1+ (more )
Structure Level Pattern Image Note
TS12: heart Present 2b , 2c

Specimen 2d: embryonic day 8.5; Slc8a1tm1Sjc/Slc8a1+ (more )
Structure Level Pattern Image Note
TS13: heart Present 2d
TS13: ectoplacental cone Absent 2d
TS13: yolk sac Absent 2d

Specimen 2e: embryonic day 11.0; Slc8a1tm1Sjc/Slc8a1+ (more )
Structure Level Pattern Image Note
TS18: heart Present 2e

Specimen 2f: embryonic day 14.0; Slc8a1tm1Sjc/Slc8a1+ (more )
Structure Level Pattern Image Note
TS22: heart Present 2f
TS22: embryo Present Regionally restricted 2f Expression was detected in the heart and several other tissues.

Specimen 2g: embryonic day 14.0; Slc8a1tm1Sjc/Slc8a1+ (more )
Structure Level Pattern Image Note
TS22: spinal cord Present Regionally restricted 2g Expression was detected in the ventral part of the neural tube.
TS22: dorsal root ganglion Present 2g
TS22: forelimb interdigital region Present 2g
TS22: limb musculature Present 2g

Specimen 2h: embryonic day 14.0; Slc8a1tm1Sjc/Slc8a1+ (more )
Structure Level Pattern Image Note
TS22: heart left ventricle Present Uniform 2h
TS22: heart right ventricle Present Uniform 2h
TS22: left atrium Present Uniform 2h
TS22: right atrium Present Uniform 2h
TS22: interventricular septum Strong Non-Uniform 2h Staining within the interventricular septum is particularly strong where the muscular component meets the atrio-ventricular bulbar cushion tissue.

Specimen 2i: postnatal month 3; Slc8a1tm1Sjc/Slc8a1+ (more )
Structure Level Pattern Image Note
TS28: heart Present Uniform 2i
TS28: lung vascular element Present Regionally restricted 2i Expression was detected within the skeletal muscle of the lung arterial vessels.
TS28: lung Present Regionally restricted 2i Expression was detected within the skeletal muscle of the lung arterial vessels, but the rest of the lung does not have staining.

Specimen 2k: postnatal adult; Slc8a1tm1Sjc/Slc8a1+ (more )
Structure Level Pattern Image Note
TS28: heart right ventricle Present 2k

Specimen 3a +/-: embryonic day 9.0; Slc8a1tm1Sjc/Slc8a1tm1Sjc (more )
Structure Level Pattern Image Note
TS14: heart Present 3a +/-

Specimen 3a -/-: embryonic day 9.0; Slc8a1tm1Sjc/Slc8a1+ (more )
Structure Level Pattern Image Note
TS14: heart Present 3a -/- Expression was stronger in the null embryo because of the presence of two copies of the reporter gene.

Specimen 3b +/-: embryonic day 9.0; Slc8a1tm1Sjc/Slc8a1tm1Sjc (more )
Structure Level Pattern Image Note
TS14: heart Present 3b +/-

Specimen 3b -/-: embryonic day 9.0; Slc8a1tm1Sjc/Slc8a1+ (more )
Structure Level Pattern Image Note
TS14: heart Present 3b -/- Expression was stronger in the null embryo because of the presence of two copies of the reporter gene.

Specimen 3c +/-: embryonic day 10.0; Slc8a1tm1Sjc/Slc8a1tm1Sjc (more )
Note: 29 somites.
Structure Level Pattern Image Note
TS16: heart Present 3c +/-

Specimen 3c -/-: embryonic day 10.0; Slc8a1tm1Sjc/Slc8a1+ (more )
Note: 29 somites.
Structure Level Pattern Image Note
TS16: heart Present 3c -/-

Specimen 3e/f +/-: embryonic day 11.0; Slc8a1tm1Sjc/Slc8a1tm1Sjc (more )
Note: 37 somites.
Structure Level Pattern Image Note
TS18: heart Present 3f , 3e +/-

Specimen 3e/g -/-: embryonic day 11.0; Slc8a1tm1Sjc/Slc8a1+ (more )
Note: 37 somites.
Structure Level Pattern Image Note
TS18: heart Present 3g , 3e -/-


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory