Gene Expression Data
Assay Details
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Assay
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Reference:
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J:81673
Mbiene JP, et al., Distribution of keratin 8-containing cell clusters in mouse embryonic tongue: Evidence for a prepattern for taste bud development. J Comp Neurol. 2003 Mar 3;457(2):111-22
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5521519
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Gene symbol:
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Nefm
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Gene name:
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neurofilament, medium polypeptide
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Antibody:
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anti-neurofilament clone 2H3
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to Cy3
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Assay notes:
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Data was summarized in Figure 4 and Table 1.
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Results
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Specimen
6A:
embryonic day 11.5
(more  )
Note: Fixed by either acid alcohol fixative (70% ethanol and 10% acetic acid) or 4% paraformaldehyde.
Specimen
6A:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Fixed by either acid alcohol fixative (70% ethanol and 10% acetic acid) or 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: cranial nerve |
Present |
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6A
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Expressed in the lingual nerve. Expressed in the major sensory nerve branches in the tongue rudiment.
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Specimen
6B:
embryonic day 12.5
(more )
Note: Fixed by either acid alcohol fixative (70% ethanol and 10% acetic acid) or 4% paraformaldehyde.
Specimen
6B:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Fixed by either acid alcohol fixative (70% ethanol and 10% acetic acid) or 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: trigeminal nerve |
Present |
Regionally restricted |
6B
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Expressed in the chorda-lingual nerve bundle, which enters the tongue base, then grow toward the dorsal epithelium. The nerve endings was restricted to two narrow bands that extended longitudinally on both sides of the midline region.
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Specimen
6C:
embryonic day 13.5
(more )
Note: Fixed by either acid alcohol fixative (70% ethanol and 10% acetic acid) or 4% paraformaldehyde.
Specimen
6C:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Fixed by either acid alcohol fixative (70% ethanol and 10% acetic acid) or 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: trigeminal nerve mandibular division |
Present |
Regionally restricted |
6C
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Expressed in the lingual nerve. Widened nerve endings to include more lateral areas. The midline region remained devoid of innervation.
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Specimen
6D:
embryonic day 13.0
(more )
Note: Fixed by either acid alcohol fixative (70% ethanol and 10% acetic acid) or 4% paraformaldehyde.
Specimen
6D:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Agarose
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Note:
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Fixed by either acid alcohol fixative (70% ethanol and 10% acetic acid) or 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: trigeminal nerve mandibular division |
Present |
Regionally restricted |
6D
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Expressed in the lingual nerve. Taste bud placodes with nerve terminals uniformly spread in the underlying connective tissue layer.
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Specimen
6E:
embryonic day 13.5
(more )
Note: Fixed by either acid alcohol fixative (70% ethanol and 10% acetic acid) or 4% paraformaldehyde.
Specimen
6E:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Agarose
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Note:
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Fixed by either acid alcohol fixative (70% ethanol and 10% acetic acid) or 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: trigeminal nerve mandibular division |
Present |
Regionally restricted |
6E
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Expressed in the lingual nerve. Numerous nerve terminals can be seen approaching the basal membrane but not contacting the taste bud placode.
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Specimen
6F:
embryonic day 14.5
(more )
Note: Fixed by either acid alcohol fixative (70% ethanol and 10% acetic acid) or 4% paraformaldehyde.
Specimen
6F:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Agarose
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Note:
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Fixed by either acid alcohol fixative (70% ethanol and 10% acetic acid) or 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: trigeminal nerve mandibular division |
Present |
Regionally restricted |
6F
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Expressed in the lingual nerve. Nerve fascicles are directed toward the gustatory papilla and terminate in a nerve plexus at the base of the developing papilla core. A few nerve fibers make contacts with the apex of the fungiform papilla.
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Specimen
6G:
embryonic day 15.5
(more )
Note: Fixed by either acid alcohol fixative (70% ethanol and 10% acetic acid) or 4% paraformaldehyde.
Specimen
6G:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Agarose
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Note:
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Fixed by either acid alcohol fixative (70% ethanol and 10% acetic acid) or 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: trigeminal nerve mandibular division |
Present |
Regionally restricted |
6G
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Expressed in the lingual nerve. Nerve fascicle is present in the papilla mesenchymal core. Nerve terminals extend into the apex and the entire developing fungiform papilla. A few nerves are seen at the base of developing filiform papillae.
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