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Gene Expression Data
Assay Details
Assay
Reference: J:81673 Mbiene JP, et al., Distribution of keratin 8-containing cell clusters in mouse embryonic tongue: Evidence for a prepattern for taste bud development. J Comp Neurol. 2003 Mar 3;457(2):111-22
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5521502
Gene symbol: Krt8
Gene name: keratin 8
Antibody: TROMA 1
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to Cy3
Assay notes: Data was summarized in Figure 4 and Table 1.
Results
Specimen 13.5 d.p.c.: embryonic day 13.5 (more )
Note: Fixed by either acid alcohol fixative (70% ethanol and 10% acetic acid) or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS21: tongue epithelium Present Regionally restricted 4_13.5 37.3% of neonatal taste buds were already present in the developing anterior tongue, if taste bud numbers normalized to 100% at birth.

Specimen 14.5 d.p.c.: embryonic day 14.5 (more )
Note: Fixed by either acid alcohol fixative (70% ethanol and 10% acetic acid) or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: fungiform papillae Present 4_14.5 69.5% of neonatal taste buds were already present in the developing anterior tongue, if taste bud numbers normalized to 100% at birth.

Specimen 15.5 d.p.c.: embryonic day 15.5 (more )
Note: Fixed by either acid alcohol fixative (70% ethanol and 10% acetic acid) or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS23: fungiform papillae Present 4_15.5 76.4% of neonatal taste buds were already present in the developing anterior tongue, if taste bud numbers normalized to 100% at birth.

Specimen 16.5 d.p.c.: embryonic day 16.5 (more )
Note: Fixed by either acid alcohol fixative (70% ethanol and 10% acetic acid) or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS24: fungiform papillae Present 4_16.5 A very slow addition of new embryonic taste bud.

Specimen 17.5 d.p.c.: embryonic day 17.5 (more )
Note: Fixed by either acid alcohol fixative (70% ethanol and 10% acetic acid) or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS25: fungiform papillae Present 4_17.5 A very slow addition of new embryonic taste bud.

Specimen 18.5 d.p.c.: embryonic day 18.5 (more )
Note: Fixed by either acid alcohol fixative (70% ethanol and 10% acetic acid) or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS26: fungiform papillae Present 4_18.5 A very slow addition of new embryonic taste bud.

Specimen birth: postnatal day 0.5 (more )
Note: Fixed by either acid alcohol fixative (70% ethanol and 10% acetic acid) or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS27: fungiform papillae Present 4_birth


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory