Antibody:	TROMA 1
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to Cy3

Structure	Level	Pattern	Image	Note
TS21: tongue epithelium	Present	Non-Uniform	1C	More intensely staining in the local thickenings of the fungiform epithelial placodes than the surrounding epithelium.

Structure	Level	Pattern	Image	Note
TS21: tongue epithelium	Present	Non-Uniform	1D	Expressed in the borders between elongated cells in the anterior tongue. Expression was particularly intense at the basal membrane and peridermal cells capping the fungiform eminence.

Structure	Level	Pattern	Image	Note
TS21: tongue epithelium	Present	Non-Uniform	1E	The circumvallate papilla placode was homogeneously and intensely labled in the posterior tongue.

Structure	Level	Pattern	Image	Note
TS21: tongue epithelium	Strong	Clusters	2A	Embryonic taste buds are intensely labeled as clusters and are organized in rows on both sides of the midline sulcus in the anterior tongue.

Structure	Level	Pattern	Image	Note
TS22: fungiform papillae	Strong	Regionally restricted	2B Top	Embryonic taste buds are more intensely labeled than the neighboring epithelial cells.

Structure	Level	Pattern	Image	Note
TS22: fungiform papillae	Strong	Regionally restricted	2B second	Embryonic taste buds are more intensely labeled than the neighboring epithelial cells.

Structure	Level	Pattern	Image	Note
TS22: fungiform papillae	Strong	Regionally restricted	2B third	Embryonic taste buds are more intensely labeled than the neighboring epithelial cells.

Structure	Level	Pattern	Image	Note
TS22: fungiform papillae	Strong	Regionally restricted	2B Bottom	Embryonic taste buds are more intensely labeled than the neighboring epithelial cells.

Structure	Level	Pattern	Image	Note
TS21: tongue epithelium	Strong	Regionally restricted	2C top	Embryonic taste buds were seen in the middle and intensely labeled, in contrast to the surrounding lingual epithelium.

Structure	Level	Pattern	Image	Note
TS21: tongue epithelium	Strong	Regionally restricted	2C middle	Embryonic taste buds were seen in the middle and intensely labeled, in contrast to the surrounding lingual epithelium.

Structure	Level	Pattern	Image	Note
TS21: tongue epithelium	Strong	Regionally restricted	2C bottom	Embryonic taste buds were seen in the middle and intensely labeled, in contrast to the surrounding lingual epithelium.

Structure	Level	Pattern	Image	Note
TS22: fungiform papillae	Strong	Regionally restricted	2D top	Embryonic taste buds were found at the apex of the dermal core and intensely labeled, in contrast to the surrounding lingual epithelium.

Structure	Level	Pattern	Image	Note
TS22: fungiform papillae	Strong	Regionally restricted	2D middle	Embryonic taste buds were found at the apex of the dermal core and intensely labeled, in contrast to the surrounding lingual epithelium.

Structure	Level	Pattern	Image	Note
TS22: fungiform papillae	Strong	Regionally restricted	2D bottom	Embryonic taste buds were found at the apex of the dermal core and intensely labeled, in contrast to the surrounding lingual epithelium.

Structure	Level	Pattern	Image	Note
TS23: fungiform papillae	Strong	Regionally restricted	2E top	Embryonic taste buds were found at the apex of the dermal core and intensely labeled, in contrast to the surrounding lingual epithelium.

Structure	Level	Pattern	Image	Note
TS23: fungiform papillae	Strong	Regionally restricted	2E middle	Embryonic taste buds were found at the apex of the dermal core and intensely labeled, in contrast to the surrounding lingual epithelium.

Structure	Level	Pattern	Image	Note
TS23: fungiform papillae	Strong	Regionally restricted	2E bottom	Embryonic taste buds were found at the apex of the dermal core and intensely labeled, in contrast to the surrounding lingual epithelium.

Structure	Level	Pattern	Image	Note
TS23: circumvallate papillae	Present	Regionally restricted	3A , 3B	Expressed in taste buds, which labeled as collections of elongated cells.

Structure	Level	Pattern	Image	Note
TS25: circumvallate papillae	Present	Regionally restricted	3C	Expressed in taste buds, which labeled as collections of elongated cells.

Structure	Level	Pattern	Image	Note
TS21: tongue epithelium	Strong	Regionally restricted	5B	Embryonic taste buds were intensely labeled, in contrast to the surrounding lingual epithelium. They are clusters of nondividing epithelial cells.

Structure	Level	Pattern	Image	Note
TS21: tongue epithelium	Strong	Regionally restricted	5E	Embryonic taste buds were intensely labeled, in contrast to the surrounding lingual epithelium. They are clusters of nondividing epithelial cells.

Structure	Level	Pattern	Image	Note
TS21: tongue epithelium	Strong	Regionally restricted	5H	Expressed throughout the entire circumvallate palate primordium in the posterior tongue. They are clusters of nondividing epithelial cells.

Structure	Level	Pattern	Image	Note
TS28: salivary gland	Present