Gene Expression Data
Assay Details
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Assay
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Reference:
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J:98304
Bonnin MA, et al., Six1 is not involved in limb tendon development, but is expressed in limb connective tissue under Shh regulation. Mech Dev. 2005 Apr;122(4):573-85
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5520179
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Gene symbol:
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Scx
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Gene name:
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scleraxis scleraxis bHLH transcription factor
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Probe:
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Scleraxis cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1B:
embryonic day 12.5
(more  )
Specimen
1B:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: foot mesenchyme |
Present |
Regionally restricted |
1B
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Expressed in the tendon primordia, underneath the ectoderm.
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Specimen
1E:
embryonic day 12.5
(more )
Specimen
1E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
1H:
embryonic day 12.5
(more )
Specimen
1H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3C/D:
embryonic day 13.5
(more )
Specimen
3C/D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3G:
embryonic day 13.5
(more )
Specimen
3G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: foot mesenchyme |
Present |
Regionally restricted |
3G
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Expressed in the tendons.
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Specimen
3J/K:
embryonic day 13.5
(more )
Specimen
3J/K:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5B:
(close )
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Genetic Background:
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involves: C57BL
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Mutant Allele(s):
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Pax3Sp/Pax3Sp
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: foot mesenchyme |
Present |
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5B
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Expression was similar to that in normal limbs.
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Specimen
5D:
(close )
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Genetic Background:
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involves: C57BL
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Mutant Allele(s):
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Pax3Sp/Pax3Sp
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
|
Embedding:
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Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: foot mesenchyme |
Present |
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5D
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Expression was similar to that in normal limbs.
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Specimen
5F:
(close )
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Genetic Background:
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involves: C57BL
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Mutant Allele(s):
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Pax3Sp/Pax3Sp
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5H:
(close )
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Genetic Background:
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involves: C57BL
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Mutant Allele(s):
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Pax3Sp/Pax3Sp
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: lower leg mesenchyme |
Weak |
Regionally restricted |
5H
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Reduced expression in tendons. Faintly expressed in a ventral tendon.
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Specimen
5J:
embryonic day 13.5
(more )
Specimen
5J:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6A:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Six1tm1Mair/Six1tm1Mair
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: foot mesenchyme |
Present |
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6A
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Expression was similar to that in normal limbs.
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Specimen
6C:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Six1tm1Mair/Six1tm1Mair
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6E:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Six1tm1Mair/Six1tm1Mair
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
not shown:
embryonic day 13.5; Pax3Sp/ Pax3Sp
(more )
Specimen
not shown:
(close )
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Genetic Background:
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involves: C57BL
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Mutant Allele(s):
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Pax3Sp/Pax3Sp
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: foot mesenchyme |
Present |
Regionally restricted |
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Expressed in distal digit tendons.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Six1tm1Mair/Six1tm1Mair
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Analysis Tools