Gene Expression Data
Assay Details
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Assay
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Reference:
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J:98304
Bonnin MA, et al., Six1 is not involved in limb tendon development, but is expressed in limb connective tissue under Shh regulation. Mech Dev. 2005 Apr;122(4):573-85
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5520096
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Gene symbol:
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Six1
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Gene name:
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sine oculis-related homeobox 1
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Probe:
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Six1 cDNA6
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1A:
embryonic day 12.5
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: foot mesenchyme |
Present |
Regionally restricted |
1A
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Expressed more closely to cartilage elements.
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Specimen
1D:
embryonic day 12.5
(more )
Specimen
1D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindlimb mesenchyme |
Present |
Regionally restricted |
1D
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Strongly expressed in the posterior mesenchyme at the ankle level. Expressed in myogenic cells of dorsal and ventral muscle masses.
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Specimen
1G:
embryonic day 12.5
(more )
Specimen
1G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: lower leg mesenchyme |
Present |
Regionally restricted |
1G
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Strongly expressed in the posterior mesenchyme. Less intense expression in the anterior. Expressed in myogenic cells of dorsal and ventral muscle masses.
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Specimen
3A/B:
embryonic day 13.5
(more )
Specimen
3A/B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: foot mesenchyme |
Present |
Regionally restricted |
3A
,
3B
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Expression was located adjacent to digit tendons.
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Specimen
3E:
embryonic day 13.5
(more )
Specimen
3E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: foot mesenchyme |
Present |
Regionally restricted |
3E
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Expression was located near cartilage regions that could correspond to ligaments in proximal autopods.
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Specimen
3H/I:
embryonic day 13.5
(more )
Specimen
3H/I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: lower leg mesenchyme |
Present |
Regionally restricted |
3H
,
3I
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Expression was located both in and around individual muscles, but not in tendons. Expression was less intense in posterior connective tissue.
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Specimen
5A:
(close )
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Genetic Background:
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involves: C57BL
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Mutant Allele(s):
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Pax3Sp/Pax3Sp
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: foot mesenchyme |
Present |
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5A
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The myogenic expression domain was clearly absent, due to the absence of myogenic cells. The non-myogenic expression domain was detected normally.
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Specimen
5C:
(close )
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Genetic Background:
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involves: C57BL
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Mutant Allele(s):
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Pax3Sp/Pax3Sp
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: foot mesenchyme |
Present |
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5C
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The myogenic expression domain was clearly absent, due to the absence of myogenic cells. The non-myogenic expression domain was detected normally.
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Specimen
5E:
(close )
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Genetic Background:
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involves: C57BL
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Mutant Allele(s):
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Pax3Sp/Pax3Sp
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: lower leg mesenchyme |
Present |
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5E
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The myogenic expression domain was clearly absent, due to the absence of myogenic cells. The non-myogenic expression domain was detected normally.
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Specimen
5G:
(close )
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Genetic Background:
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involves: C57BL
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Mutant Allele(s):
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Pax3Sp/Pax3Sp
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: lower leg mesenchyme |
Present |
Regionally restricted |
5G
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The myogenic expression domain was clearly absent, due to the absence of myogenic cells. The non-myogenic expression domain was detected normally.
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Specimen
5I:
embryonic day 13.5
(more )
Specimen
5I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
7F:
embryonic day 11.5
(more )
Specimen
7F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindlimb bud mesenchyme |
Present |
Regionally restricted |
7F
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Expressed in the posterior limb regions. Expression domain does not cover all of the posterior regions.
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Specimen
not shown:
embryonic day 18.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: limb mesenchyme |
Present |
Regionally restricted |
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No expression in limb tendons. Expression sites were close to digit tendons.
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Analysis Tools