Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present		2A
TS19: inner ear	Present		2A
TS19: mammary placode	Present		2A
TS19: surface ectoderm	Weak	Regionally restricted	2A	Faint expression was detected in vibrissae buds.
TS19: limb mesenchyme	Weak	Regionally restricted	2A	Faint expression was detected in mesenchymal condensations of future endochondral bones in the upper extremity.
TS19: head mesenchyme	Weak	Regionally restricted	2A	Faint expression was detected in mesenchymal condensations of the basicranium.

Structure	Level	Pattern	Image	Note
TS21: olfactory epithelium	Present		2B
TS21: vibrissa follicle	Present		2B
TS21: limb mesenchyme	Present	Regionally restricted	2B	Expression was detected in the mesenchymal condensations of future long bones.

Structure	Level	Pattern	Image	Note
TS22: foot	Present		2D
TS22: inner ear	Present		2D
TS22: lower jaw incisor	Present		2D
TS22: rib cartilage condensation	Present		2D

Structure	Level	Pattern	Image	Note
TS23: hair follicle	Present		2F , 2E
TS23: integumental system	Present	Regionally restricted	2F , 2E	Expression was detected in hair follicle buds, but the interfollicular periderm was not stained.

Structure	Level	Pattern	Image	Note
TS24: skeleton	Present	Regionally restricted	2G	Expression was detected in the endochondral skeleton.
TS24: lower jaw incisor	Present		2G , 2H
TS24: semicircular canal	Present		2G , 2H
TS24: arterial system	Present	Regionally restricted	2H , 2G	Staining was noted in several crisscrossing arteries in front of the ear.

Structure	Level	Pattern	Image	Note
TS27: skeletal system	Present	Regionally restricted	3A	Expression was detected in the endochondral skeleton.
TS27: cartilage	Present	Regionally restricted	3A
TS27: rib	Present		3A
TS27: forelimb long bone	Present	Regionally restricted	3A	Expression was detected in well-defined zones of activity at each end of the proximal and distal long bones.
TS27: hindlimb long bone	Present	Regionally restricted	3A	Expression was detected in well-defined zones of activity at each end of the proximal and distal long bones.
TS27: pelvic region of trunk	Present	Regionally restricted	3A	Expression was detected in the forming growth plate.
TS27: scapula	Present	Regionally restricted	3A	Expression was detected in the forming growth plate.
TS27: tail vertebra	Present	Regionally restricted	3A	Expression was detected in the individual cartilaginous components of the vertebrae.
TS27: vertebra	Present	Regionally restricted	3A	Expression was detected in the individual cartilaginous components of the vertebrae.

Structure	Level	Pattern	Image	Note
TS27: rib	Present	Regionally restricted	3B	Expression was in the proximal portions of the forming growth plates.

Structure	Level	Pattern	Image	Note
TS27: cranium	Present		3C

Structure	Level	Pattern	Image	Note
TS28: femur	Present	Regionally restricted	3D	Expression was detected in the head of the femur.

Structure	Level	Pattern	Image	Note
TS23: rib	Present	Regionally restricted	3E	Expression was detected throughout the length of the costal cartilage.

Structure	Level	Pattern	Image	Note
TS22: tibia cartilage condensation	Present	Regionally restricted	4A	Expression is polarized at the proximal end.

Structure	Level	Pattern	Image	Note
TS24: tibia	Present	Regionally restricted	4B	Expression in the proximal tibia remains concentrated in the subarticular region. It is not uniform in its horizontal distribution. Staining does not extend to the more distal proliferative zone or into the columnar proliferative chondrocyte population.

Structure	Level	Pattern	Image	Note
TS28: tibia	Present	Regionally restricted	4F , 4E , 4C	Two separate and distinct chondrocyte populations were noted. The upper proliferative chondrocytes (higher mag in 4E), and the subarticular chondrocytes (higher mag in 4F). The periosteum was stained, but the primary spongiosa was not.

Structure	Level	Pattern	Image	Note
TS28: tibia	Present	Regionally restricted	4D , 4G , 4H	Staining in the two chondrocyte populations continued. The growth plate proliferative chondrocytes (higher mag in 4G), and the subarticular chondrocytes (higher mag in 4H).

Structure	Level	Pattern	Image	Note
TS28: connective tissue	Strong	Regionally restricted	5A	Expression was detected at the fibrous tendon insertion sites in the femur.

Structure	Level	Pattern	Image	Note
TS28: connective tissue	Strong	Regionally restricted	5B	Expression was detected at fibrous sites of periosteal type muscle insertions along the posterolateral side of the tibia.
TS28: periosteum	Present	Regionally restricted	5B	Expression was detected in the periosteal flap.

Structure	Level	Pattern	Image	Note
TS28: connective tissue	Present	Regionally restricted	5C	Expression was detected in an intermediate zone between the dense connective tissue of the tendon and the connective tissue that interdigitates with the mineralized cortical bone.

Structure	Level	Pattern	Image	Note
TS28: connective tissue	Present	Regionally restricted	5D	Expression was detected in an intermediate layer of cells between the muscle cells and the cortical bone.

Structure	Level	Pattern	Image	Note
TS28: periosteum	Present	Regionally restricted	5E	Expression was detected in the most peripheral or fibrous layer of periosteal cells. No staining was detected in the inner or cambial layer.

Structure	Level	Pattern	Image	Note
TS26: knee	Present		6B
TS26: rib	Present	Regionally restricted	6B	Expression was detected in costal cartilage.

Structure	Level	Pattern	Image	Note
TS26: knee	Present		6C	Staining was more extensive in the homozygous embryo compared to the heterozygous embryo.
TS26: rib	Present	Regionally restricted	6C	Staining was more extensive in the costal cartilage of the homozygous embryo compared to the heterozygous embryo.

Structure	Level	Pattern	Image	Note
TS26: tibia	Present	Regionally restricted	6E	Expression was detected in subarticular chondrocytes.

Structure	Level	Pattern	Image	Note
TS26: tibia	Present	Regionally restricted	6F	There was a dramatic expansion of the beta-gal+ population of subarticular chondrocytes in the homozygous embryo.