Gene Expression Data
Assay Details
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Assay
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Reference:
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J:136995
Tashiro Y, et al., Development and migration of GABAergic neurons in the mouse myelencephalon. J Comp Neurol. 2007 Jul 10;503(2):260-9
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5515342
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Gene symbol:
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Gad1
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Gene name:
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glutamate decarboxylase 1
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Reporter:
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GFP
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Antibody:
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anti-GFP
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Detection system:
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Secondary antibody coupled to Cy2
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Results
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Specimen
1E,I,J:
(close  )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Gad1tm1.1Tama/Gad1+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
1J
,
1I
,
1E
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Expression is in the ventral and intermediate neural tube mostly beneath the pial surface.
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Specimen
1F:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Gad1tm1.1Tama/Gad1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: myelencephalon |
Present |
Regionally restricted |
1F
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Expression is in the ventral and intermediate neural tube. Absent in the dorsalmost region and in a narrow region between the ventral and dorsal part of the neural tube.
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Specimen
1G:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Gad1tm1.1Tama/Gad1+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: medulla oblongata |
Present |
Regionally restricted |
1G
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Expression is in a wide area except for a narrow area between the ventral and dorsal neural tube.
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Specimen
1H:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Gad1tm1.1Tama/Gad1+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: medulla oblongata |
Strong |
Regionally restricted |
1H
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Expression is mainly dorsal except in the ventrolateral area.
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Specimen
2A:
embryonic day 10.5; Gad1tm1.1Tama/Gad1 +
(more  )
Note: Flat mount.
Specimen
2A:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Gad1tm1.1Tama/Gad1+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Flat mount.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
2A
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Expression is in two longitudinal columns separated by a thin column with weak signals along the entire length of the ventral myelencephalon.
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Specimen
2B:
embryonic day 11.5; Gad1tm1.1Tama/Gad1 +
(more )
Note: Flat mount.
Specimen
2B:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Gad1tm1.1Tama/Gad1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Flat mount.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindbrain |
Strong |
Regionally restricted |
2B
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Expression is in two longitudinal columnbs.
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Specimen
2C:
embryonic day 12.5; Gad1tm1.1Tama/Gad1 +
(more )
Note: Flat mount.
Specimen
2C:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Gad1tm1.1Tama/Gad1+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Flat mount.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindbrain |
Present |
Regionally restricted |
2C
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Expression is in all areas except for a narrow area between the ventral and dorsal neural tube.
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Analysis Tools
