Gene Expression Data
Assay Details
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Assay
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Reference:
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J:56184
North T, et al., Cbfa2 is required for the formation of intra-aortic hematopoietic clusters. Development. 1999 Jun;126(11):2563-75
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|
Assay type:
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In situ reporter (knock in)
|
|
MGI Accession ID:
|
MGI:5514253
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|
Gene symbol:
|
Runx1
|
|
Gene name:
|
runt related transcription factor 1
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|
Reporter:
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lacZ
|
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|
Direct Detection
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|
Assay notes:
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Semi-thin sections from AGM regions of E10.5 embryos were fixed in 3% glutaraldehyde. Fixative for other specimens was not specified.
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Results
|
Specimen
2A:
embryonic day 7.5; Runx1tm2Spe/Runx1 +
(more  )
Specimen
2A:
(close  )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
|
Age:
|
embryonic day 7.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
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Fixation:
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Other - See Notes
|
Specimen
not shown E8.0:
embryonic day 8.0; Runx1tm2Spe/Runx1 +
(more )
Specimen
not shown E8.0:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: yolk sac |
Present |
Regionally restricted |
|
Expression is in newly emerging primitive erythrocytes in the yolk sac.
|
Specimen
not shown E8.5:
embryonic day 8.5; Runx1tm2Spe/Runx1 +
(more )
Specimen
not shown E8.5:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
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Type:
|
whole mount
|
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: allantois |
Present |
Regionally restricted |
|
Expression is in mesenchymal cells in the distal portion of the allantois at the point where it joins the chorion.
|
Specimen
2B:
embryonic day 8.5; Runx1tm2Spe/Runx1 +
(more )
Specimen
2B:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
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|
Type:
|
whole mount
|
|
Fixation:
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Other - See Notes
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: yolk sac |
Present |
Regionally restricted |
2B
|
Expression is in the blood islands as a tight band encircling the yolk sac.
|
Specimen
2C:
embryonic day 8.5; Runx1tm2Spe/Runx1 +
(more )
Specimen
2C:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
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Other - See Notes
|
|
Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: yolk sac |
Present |
Regionally restricted |
2C
|
Expression is in endothelial cells, and in hematopoietic cells closely associated with the endothelium, primitive erythrocytes express low levels.
|
| TS13: yolk sac endoderm |
Present |
Punctate |
2C
|
|
Specimen
2D:
embryonic day 8.5; Runx1tm2Spe/Runx1 +
(more )
Specimen
2D:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
2E:
embryonic day 10.5; Runx1tm2Spe/Runx1 +
(more )
Specimen
2E:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
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Other - See Notes
|
Specimen
2F:
embryonic day 10.5; Runx1tm2Spe/Runx1 +
(more )
Specimen
2F:
(close )
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Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
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Other - See Notes
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Embedding:
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Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: vitelline artery |
Present |
Regionally restricted |
2F
|
Expression is in a grape-like cluster of hematopoietic cells.
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Specimen
3A:
embryonic day 10.5; Runx1tm2Spe/Runx1 +
(more )
Specimen
3A:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
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Fixation:
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Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: dorsal aorta |
Present |
Regionally restricted |
3A
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Expression is in the ventral wall within the AGM region.
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| TS17: liver |
Present |
Regionally restricted |
3A
|
Expression is in hematopoietic cells.
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Specimen
3B:
embryonic day 10.5; Runx1tm2Spe/Runx1 +
(more )
Specimen
3B:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
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Other - See Notes
|
|
Embedding:
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Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: aorta-gonad-mesonephros |
Present |
Regionally restricted |
3B
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Expression is in the ventral para-aortic mesenchyme within the AGM region.
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Specimen
3C:
embryonic day 10.5; Runx1tm2Spe/Runx1 +
(more )
Specimen
3C:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: dorsal aorta |
Present |
Regionally restricted |
3C
|
Expression is in some endothelial cells, para-aortic mesenchyme, and in circulating hematopoietic cells. Absent in most circulating blood cells (primitive erythrocytes).
|
Specimen
3D:
embryonic day 14.5; Runx1tm2Spe/Runx1 +
(more )
Specimen
3D:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: dorsal aorta |
Present |
Regionally restricted |
3D
|
Expression is in the para-aortic mesenchyme, absent in the endothelium.
|
Specimen
not shown E17.5:
embryonic day 17.5; Runx1tm2Spe/Runx1 +
(more )
Specimen
not shown E17.5:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
|
Age:
|
embryonic day 17.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: umbilical cord |
Present |
Regionally restricted |
|
Expression is in the mesenchyme.
|
Specimen
5A:
embryonic day 10.5; Runx1tm2Spe/Runx1 +
(more )
Specimen
5A:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: blood |
Present |
Regionally restricted |
5A
|
Expression is in hematopoietic cells within the liver sinusoids and in the liver capsule mesoderm.
|
| TS17: liver |
Present |
Regionally restricted |
5A
|
Expression is in hematopoietic cells within the liver sinusoids and in the liver capsule mesoderm.
|
Specimen
5B:
(close )
|
Genetic Background:
|
either: (involves: 129S4/SvJae * BALB/c) or (involves: 129S4/SvJae * C57BL/6)
|
|
Mutant Allele(s):
|
Runx1tm1Spe/Runx1tm2Spe
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: liver |
Present |
Regionally restricted |
5B
|
Expression is in the liver capsule mesoderm and is absent in hematopoietic cells within the liver sinusoids.
|
| TS17: blood |
Absent |
|
5B
|
Expression is absent in hematopoietic cells within the liver sinusoids
|
Specimen
5C:
embryonic day 9.5; Runx1tm2Spe/Runx1 +
(more )
Specimen
5C:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: vitelline artery |
Present |
Regionally restricted |
5C
|
Expression is in endothelial cells.
|
Specimen
5D:
(close )
|
Genetic Background:
|
either: (involves: 129S4/SvJae * BALB/c) or (involves: 129S4/SvJae * C57BL/6)
|
|
Mutant Allele(s):
|
Runx1tm1Spe/Runx1tm2Spe
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: vitelline artery |
Present |
Regionally restricted |
5D
|
Expression is in endothelial cells.
|
Specimen
5E:
embryonic day 10.0; Runx1tm2Spe/Runx1 +
(more )
Specimen
5E:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
|
Age:
|
embryonic day 10.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: vitelline artery |
Strong |
Regionally restricted |
5E
|
Expression in in both endothelial cells and hematopoietic clusters.
|
Specimen
5F:
(close )
|
Genetic Background:
|
either: (involves: 129S4/SvJae * BALB/c) or (involves: 129S4/SvJae * C57BL/6)
|
|
Mutant Allele(s):
|
Runx1tm1Spe/Runx1tm2Spe
|
|
Age:
|
embryonic day 10.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: vitelline artery |
Trace |
Regionally restricted |
5F
|
Expression in in endothelial cells and is absent in hematopoietic clusters.
|
Specimen
5G:
embryonic day 9.5; Runx1tm2Spe/Runx1 +
(more )
Specimen
5G:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: yolk sac |
Strong |
Regionally restricted |
5G
|
Expression is in endothelial and hematopoietic cells.
|
Specimen
5H:
(close )
|
Genetic Background:
|
either: (involves: 129S4/SvJae * BALB/c) or (involves: 129S4/SvJae * C57BL/6)
|
|
Mutant Allele(s):
|
Runx1tm1Spe/Runx1tm2Spe
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
Specimen
7A:
embryonic day 9.5; Runx1tm2Spe/Runx1 +
(more )
Specimen
7A:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: dorsal aorta |
Present |
Regionally restricted |
7A
|
Expression is in endothelial cells in the floor of the dorsal aorta.
|
Specimen
7B:
(close )
|
Genetic Background:
|
either: (involves: 129S4/SvJae * BALB/c) or (involves: 129S4/SvJae * C57BL/6)
|
|
Mutant Allele(s):
|
Runx1tm1Spe/Runx1tm2Spe
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: dorsal aorta |
Present |
Regionally restricted |
7B
|
Expression is in endothelial cells in the floor of the dorsal aorta.
|
Specimen
7C:
embryonic day 10.0; Runx1tm2Spe/Runx1 +
(more )
Specimen
7C:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
|
Age:
|
embryonic day 10.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: dorsal aorta |
Present |
Regionally restricted |
7C
|
Expression is in the paraaortic mesenchyme and in endothelial cells.
|
Specimen
7D:
(close )
|
Genetic Background:
|
either: (involves: 129S4/SvJae * BALB/c) or (involves: 129S4/SvJae * C57BL/6)
|
|
Mutant Allele(s):
|
Runx1tm1Spe/Runx1tm2Spe
|
|
Age:
|
embryonic day 10.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: dorsal aorta |
Present |
Regionally restricted |
7D
|
Expression is in the paraaortic mesenchyme and is weak in endothelial cells.
|
|
Analysis Tools
