Reporter:	lacZ
	Direct Detection
Assay notes:	Whole embryos, newborn mice, and bone tissues from post-natal mice were fixed in 2% paraformaldehyde, 0.2% glutaraldehyde. Soft tissues from adult mice were fixed in 1% formalin.

Structure	Level	Pattern	Image	Note
TS27: chondrocranium	Present	Regionally restricted	2a	Expression is in the calvarial suture tissues separating the frontal and parietal bone areas.
TS27: hair follicle	Present		2a
TS27: jaw	Present		2a
TS27: long bone epiphysis	Present		2a
TS27: scapula	Present		2a
TS27: vibrissa	Present		2a
TS27: vertebra	Present		2b , 2a
TS27: vertebral column	Present		2b , 2a
TS27: rib	Strong		2b , 2c , 2a

Structure	Level	Pattern	Image	Note
TS27: thymus	Strong		2d
TS27: appendicular skeleton	Present		2d
TS27: axial skeleton	Present		2d
TS27: lung	Present		2d
TS27: mandible	Present		2d
TS27: premaxilla	Present		2d
TS27: trachea	Present		2d
TS27: nose	Present	Regionally restricted	2d	Expression is in the soft tissues of the nasal area.
TS27: brain	Present	Regionally restricted	2d
TS27: heart	Absent		2d

Structure	Level	Pattern	Image	Note
TS27: bone marrow	Strong		2e
TS27: skin	Strong		2e
TS27: perichondrium	Strong	Regionally restricted	2e	Expression is in the perichondrium surrounding the digits and bone.
TS27: cartilage	Absent		2e	Absent in mature cartilage.

Structure	Level	Pattern	Image	Note
TS27: thyroid gland	Present

Structure	Level	Pattern	Image	Note
TS20: rib pre-cartilage condensation	Present	Regionally restricted	3a	Expression coincides with chondroprogenitors forming the cartilage anlagen of the axial and appendicular skeleton and it declined in regions of tissues undergoing endochondral bone formation.

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Very strong		3c
TS22: vertebral pre-cartilage condensation	Present	Regionally restricted	3c	Expression coincides with chondroprogenitors forming the cartilage anlagen of the axial and appendicular skeleton and it declined in regions of tissues undergoing endochondral bone formation.

Structure	Level	Pattern	Image	Note
TS24: rib	Present	Regionally restricted	3e	Expression coincides with chondroprogenitors forming the cartilage anlagen of the axial and appendicular skeleton and it declined in regions of tissues undergoing endochondral bone formation.

Structure	Level	Pattern	Image	Note
TS24: vertebra	Present	Regionally restricted	3g	Expression is in the perichondrium or periosteum of the maturing vertebral bodies.
TS24: vertebral cartilage condensation	Absent		3g	Absent in hyaline cartilage.

Structure	Level	Pattern	Image	Note
TS28: rib	Present		4a
TS28: periosteum	Present		4f , 4a
TS28: perichondrium	Present		4a , 4d	Expression is in differentiating chondroblasts from the perichondrium and in chondrocytes in lacunae in resting cartilage, absent in chondrocytes in the proliferating zone.
TS28: endochondral bone	Present	Regionally restricted	4a , 4f , 4d	Expression is in chondroprogenitors cells and early stage differentiating chondrocytes, absent in chondrocytes in the proliferating zone.
TS28: bone marrow	Present		4a
TS28: hypertrophic cartilage zone	Absent		4e , 4f

Structure	Level	Pattern	Image	Note
TS28: perichondrium	Present		4b	Expression is in differentiating chondroblasts from the perichondrium.

Structure	Level	Pattern	Image	Note
TS27: bone marrow	Present		4g
TS27: rib	Present		4g
TS27: hypertrophic cartilage zone	Absent		4g

Structure	Level	Pattern	Image	Note
TS27: perichondrium	Present		4i	Expression is present in chondrocytes.
TS27: rib	Present		4i	Expression is present in chondrocytes.

Structure	Level	Pattern	Image	Note
TS28: bone marrow	Present		5a
TS28: periosteum	Present		5a
TS28: bone	Present	Regionally restricted	5a	Expression is in early stage osteoblasts.

Structure	Level	Pattern	Image	Note
TS28: bone marrow	Present		5b
TS28: bone	Present	Regionally restricted	5b	Expression is present in osteoblasts but not osteocytes.

Structure	Level	Pattern	Image	Note
TS28: bone	Present	Regionally restricted	5c	Expression is in osteoblasts on the endosteal side of the diaphysis and in some areas within the cortical bone. It is not present in osteocytes.

Structure	Level	Pattern	Image	Note
TS28: chondrocranium	Present	Regionally restricted	6a	Expression is in suture lines of calvaria.

Structure	Level	Pattern	Image	Note
TS28: bone marrow	Present	Regionally restricted	6b	Expression is in the marrow and periosteum of all bones (shown rib).
TS28: periosteum	Present	Regionally restricted	6b	Expression is in the marrow and periosteum of all bones (shown rib).
TS28: rib	Present	Regionally restricted	6b	Expression is in the marrow and periosteum of all bones (shown rib).
TS28: sternum	Present	Regionally restricted	6b	Expression is in xiphoid cartilage.

Structure	Level	Pattern	Image	Note
TS28: bone marrow	Present	Regionally restricted	6c	Expression is in the marrow and periosteum of all bones (shown tail vertebrae).
TS28: periosteum	Present	Regionally restricted	6c	Expression is in the marrow and periosteum of all bones (shown tail vertebrae).
TS28: vertebra	Present	Regionally restricted	6c	Expression is in the marrow and periosteum of all bones (shown tail vertebrae).

Structure	Level	Pattern	Image	Note
TS28: bone marrow	Present		6d
TS28: limb long bone	Present		6d

Structure	Level	Pattern	Image	Note
TS28: periosteum	Present		6e

Structure	Level	Pattern	Image	Note
TS28: trachea cartilage	Present		6g

Structure	Level	Pattern	Image	Note
TS28: trachea cartilage	Present		6h
TS28: trachea epithelium	Present		6h

Structure	Level	Pattern	Image	Note
TS28: bronchus epithelium	Present		6i

Structure	Level	Pattern	Image	Note
TS28: respiratory bronchiole epithelium	Present		6j
TS28: alveolar duct	Absent		6j