Probe:	NeuroD cDNA7
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS21: olfactory lobe	Present	Regionally restricted	2A'	Expression was detected in the olfactory bulb.
TS21: cerebral cortex	Present	Widespread	2A'
TS21: diencephalon	Present	Regionally restricted	2A'	Expression was detected in the eminentia thalami. The basal plate posterior diencephalon showed expression in prosomeres 2 and 3, but not prosomere 1.
TS21: preoptic area	Absent		2A'
TS21: telencephalon	Present	Regionally restricted	2A'	Expression was detected in the cortex, but not in the septum.
TS21: lateral ganglionic eminence	Absent		2A'
TS21: medial ganglionic eminence	Absent		2A'
TS21: thalamus	Strong	Regionally restricted	2A'	There was strong expression in the dorsal thalamus alar plate, but no signal in the ventral thalamus. Expression was detected in the zona limitans intrathalamica.
TS21: midbrain	Strong	Regionally restricted	2A'	Strong expression was in the pretectum. Signals in the precommissural and commissural parts of the pretectum were separated by an expression-free gap. Expression was in the basal plate in the tegmentum, and longitudinally in the superior colliculus.
TS21: hypothalamus	Ambiguous		2A'	The hypothalamus was mostly free of expression.
TS21: midbrain roof plate	Present	Regionally restricted	2A'	There was a longitudinal expression domain in the midline roof plate.
TS21: midbrain-hindbrain junction	Absent		2A'
TS21: hindbrain alar plate	Present	Regionally restricted	2A'	Expression in the rhombencephalic alar plate started as a broad band in the isthmic region and thinned out more posteriorly.
TS21: hindbrain basal plate	Present	Regionally restricted	2A'	There was a longitudinal stripe of expression in the rhombencephalic basal plate.
TS21: cerebellum	Strong		2A'

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Strong		4A
TS21: midbrain roof plate	Strong	Regionally restricted	4A	The longitudinal expression domain in the midbrain roof plate was strong in the most posterior part of the midbrain.
TS21: hindbrain alar plate	Present		4A	Expression in the rhombencephalic alar plate started as a broad band in the isthmic region and thinned out more posteriorly.

Structure	Level	Pattern	Image	Note
TS21: hindbrain alar plate	Present		4B
TS21: hindbrain basal plate	Present		4B
TS21: cerebellum	Strong		4B
TS21: rhombic lip	Absent		4B

Structure	Level	Pattern	Image	Note
TS21: caudal ganglionic eminence	Absent		6F
TS21: midbrain	Strong	Regionally restricted	6F	Strong expression was in the pretectum. Signal in the precommissural and commissural parts of the pretectum were separated by an expression-free gap.
TS21: hypothalamus	Present	Regionally restricted	6F	Expression was detected in a ventriculopially extending band in the alar hypothalamus.