About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:56939 Miquerol L, et al., Multiple developmental roles of VEGF suggested by a LacZ-tagged allele. Dev Biol. 1999 Aug 15;212(2):307-22
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:5511003
Gene symbol: Vegfa
Gene name: vascular endothelial growth factor A
Reporter: lacZ
Direct Detection
Assay notes: Fixative was 0.2% glutaraldehyde, 2% paraformaldehyde and stained embryos (<E10.5) were postfixed in 4% paraformaldehyde and embedded in paraffin. Later stage embryos (>E10.5) were fixed 4% paraformaldehyde, embedded in Tissue Tek (OCT compound), frozen and cryosectioned.
Results
Specimen 2A: embryonic day 3.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS4: blastocyst Absent 2A
Specimen 2B: embryonic day 4.0; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS6: primitive endoderm Present 2B
Specimen 2C: embryonic day 4.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS6: primitive endoderm Present 2C
Specimen 2D: embryonic day 5.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS8: extraembryonic visceral endoderm Strong 2D
TS8: parietal endoderm Strong 2D
TS8: primary trophoblast giant cell Present 2D
TS8: extraembryonic ectoderm Weak 2D
Specimen 2E: embryonic day 6.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS9: extraembryonic ectoderm Weak 2E
TS9: parietal endoderm Weak 2E
Specimen 2F: embryonic day 7.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS11: extraembryonic component Strong 2F
TS11: embryo Absent 2F
Specimen 2G: embryonic day 7.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS11: extraembryonic visceral endoderm Present 2G
TS11: yolk sac mesoderm Present 2G
TS11: blood island Absent 2G
TS11: chorion Absent 2G
TS11: ectoplacental cone Absent 2G
Specimen 2H: embryonic day 8.0; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS12: allantois Weak Single cells 2H
Specimen 2I: embryonic day 8.0; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS12: extraembryonic visceral endoderm Present 2I
TS12: yolk sac mesenchyme Present 2I
TS12: blood island Absent 2I
Specimen 3D: embryonic day 8.0; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS12: foregut diverticulum Present Regionally restricted 3D Expression is in the definitive endoderm that forms the foregut pocket.
TS12: hindgut diverticulum Present Regionally restricted 3D Expression is in the definitive endoderm that forms the hindgut diverticulum.
Specimen 3E: embryonic day 8.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS13: future brain neural fold Present 3E
TS13: gut Present 3E
TS13: heart Present 3E
Specimen 3F: embryonic day 9.0; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS14: embryo Present Widespread 3F
TS14: neural tube Present 3F
Specimen 3G: embryonic day 8.0; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS12: foregut diverticulum Present Regionally restricted 3G Expression is in the definitive endoderm that forms the foregut pocket.
TS12: hindgut diverticulum Present Regionally restricted 3G Expression is in the definitive endoderm that forms the hindgut diverticulum.
Specimen 3H: embryonic day 8.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS13: gut endoderm Strong 3H
TS13: heart Moderate Regionally restricted 3H Expression is in the endocardium.
TS13: cardiac muscle tissue Moderate 3H
TS13: head mesenchyme Moderate 3H
Specimen 3I: embryonic day 9.0; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS14: neural tube Present 3I
Specimen 4A: embryonic day 8.0; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS12: cardiogenic plate Trace 4A
Specimen 4B: embryonic day 8.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS13: cardiac muscle tissue Strong 4B
TS13: heart Strong Regionally restricted 4B Expression is in part of the endocardium.
Specimen 4C: embryonic day 9.0; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS14: bulbus cordis Present 4C
TS14: endocardial tube Present 4C
TS14: sinus venosus Present 4C
TS14: arterial system Present Regionally restricted 4C Expression is in the ventral aorta and in the aortic sac.
Specimen 4D: embryonic day 9.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS15: atrioventricular canal Present Regionally restricted 4D Expression is in endocardial cells.
TS15: outflow tract Present Regionally restricted 4D Expression is in endocardial cells.
Specimen 4E: embryonic day 9.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS15: atrioventricular canal Present Regionally restricted 4E Expression is in endocardial cells.
TS15: outflow tract Present Regionally restricted 4E Expression is in endocardial cells.
Specimen 4F: embryonic day 9.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS15: atrioventricular canal Present Regionally restricted 4F Expression is in endocardial cells.
TS15: outflow tract Present Regionally restricted 4F Expression is in endocardial cells.
TS15: cardiac muscle tissue Present Regionally restricted 4F Expression is in compact and trabecular layers, and there a higher level of expression is seen in the septum transversum surrounding the atrioventricular canal.
Specimen 4G: embryonic day 9.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS15: arterial system Strong Regionally restricted 4G Expression is in endocardial cells lining the aortic sac.
TS15: endocardial cushion Strong 4G Expression is in cells lining the cushions and the endocardial cells that have undergone mesenchymal transition to form the cushion are negative for expression.
Specimen 4H: embryonic day 9.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS15: endocardial cushion Present Regionally restricted 4H Expression is in the cushion borders of the truncus arteriosis.
Specimen 4I: embryonic day 9.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS15: atrioventricular canal Present 4I
Specimen 4J: embryonic day 14.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS22: heart ventricle Strong 4J
TS22: heart atrium Weak 4J
Specimen 4K: embryonic day 14.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS22: aortico-pulmonary spiral septum Strong 4K
TS22: cardiac muscle tissue Strong 4K
TS22: interventricular septum Strong 4K
TS22: outflow tract Present Regionally restricted 4K Expression is in the endothelium.
TS22: dorsal aorta Present Regionally restricted 4K Expression is at the site of emergence from the heart and declines with increasing distance from the heart.
TS22: atrium endocardial lining Absent 4K
TS22: bulbar cushion Absent 4K
TS22: ventricle endocardial lining Absent 4K
Specimen 5A: embryonic day 14.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS22: embryo Present Widespread 5A
Specimen 5B: embryonic day 14.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS22: embryo Strong Regionally restricted 5B Strong staining in some organs and no staining in others.
TS22: intestine Strong 5B
TS22: stomach Strong 5B
Specimen 5C: embryonic day 14.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS22: embryo Strong Regionally restricted 5C Strong staining in some organs and no staining in others.
Specimen 5D: embryonic day 14.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS22: collecting duct Present Regionally restricted 5D Expression is in the epithelium of the uerteric bud.
TS22: mesenchyme of metanephros Present Regionally restricted 5D Expression is in the mesenchyme surrounding the uerteric bud.
TS22: maturing glomerular tuft Present Regionally restricted 5D Expression is in podocytes.
TS22: renal cortex Absent 5D Absent in the outer cortex.
Specimen 5F: embryonic day 14.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS22: brain Moderate Regionally restricted 5F Expression is in the ventral layer of the neuroepithelium.
Specimen 5G: embryonic day 14.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS22: blood vessel Strong Regionally restricted 5G Expression is in newly formed blood vessels that penetrate the neuroepithelium perpendicularly and grow forward towards the strongly-positive ventral layer.
TS22: brain Strong Regionally restricted 5G Expression is in newly formed blood vessels that penetrate the neuroepithelium perpendicularly and grow forward towards the strongly-positive ventral layer.
Specimen 5H: embryonic day 14.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS22: lung Strong Uniform 5H
Specimen 5J: embryonic day 14.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS22: spleen primordium Strong Uniform 5J
Specimen 6A: embryonic day 14.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS22: adrenal gland Present 6A
Specimen 6B: embryonic day 14.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS22: choroid plexus Present Regionally restricted 6B Expression is in the epithelial cells.
Specimen 6C: embryonic day 14.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS22: maturing glomerular tuft Present Regionally restricted 6C Expression is in podocytes.
Specimen 6D: embryonic day 14.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS22: stomach Present 6D
Specimen 6I: embryonic day 14.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS22: vibrissa dermal component Present 6I
TS22: vibrissa epidermal component Absent 6I Absent in the hair bulb.
Specimen 6J: embryonic day 14.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS22: mammary gland primordium epithelium Present 6J
TS22: mammary mesenchyme Absent 6J
Specimen 6K: embryonic day 14.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS22: neurohypophysis Present 6K
TS22: adenohypophysis Present 6K Expression is in the pars distalis.
TS22: adenohypophysis pars intermedia Absent 6K
Specimen 6L: embryonic day 14.5; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS22: enamel organ Strong 6L
TS22: dental lamina Present 6L
TS22: dental sac Present 6L
TS22: dental papilla Absent 6L
Specimen 7A: postnatal; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS28: ovary stratum granulosum Present 7A
Specimen 7B: postnatal; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS28: ovary stratum granulosum Present 7B
Specimen 7C: postnatal; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS28: ovary stratum granulosum Present 7C
Specimen 7D: postnatal; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS28: ovary stratum granulosum Present 7D
Specimen 7E: postnatal; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS28: corpus luteum Strong 7E
Specimen 7F: postnatal; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS28: corpus luteum Strong 7F
Specimen 7G: postnatal; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS28: corpus luteum Strong 7G
Specimen 7H: postnatal; Vegfatm1.1Nagy/Vegfa+ (more )
Structure Level Pattern Image Note
TS28: corpus luteum Strong 7H
Specimen 7I: postnatal; Vegfatm1.1Nagy/Vegfa+ (more )
Note: Staining of decidual tissue of gestational day E7.5.
Structure Level Pattern Image Note
TS28: blood vessel Present Regionally restricted 7I Expression is in newly formed blood vessels.
TS28: decidua Present Regionally restricted 7I Expression is in newly formed blood vessels.
Specimen 7J: postnatal; Vegfatm1.1Nagy/Vegfa+ (more )
Note: Staining of decidual tissue of gestational day E9.5.
Structure Level Pattern Image Note
TS28: blood vessel Present Regionally restricted 7J Expression is in newly formed blood vessels.
TS28: decidua Present Regionally restricted 7J Expression is in newly formed blood vessels.
Specimen 7K: postnatal; Vegfatm1.1Nagy/Vegfa+ (more )
Note: Staining of decidual tissue of gestational day E7.5.
Structure Level Pattern Image Note
TS28: decidua Strong 7K
TS28: endometrium Absent 7K
Specimen 7L: postnatal; Vegfatm1.1Nagy/Vegfa+ (more )
Note: Staining of decidual tissue of gestational day E7.5.
Structure Level Pattern Image Note
TS28: decidua Strong 7L
TS28: endometrium Absent 7L
TS28: myometrium Absent 7L
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory