Probe:	Prnp cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex ventricular layer	Present		1B
TS22: cortical plate	Present		1B
TS22: cerebral cortex	Present	Regionally restricted	1B	Expression is underneath the marginal zone.
TS22: cerebral cortex subventricular zone	Weak		1B
TS22: cortical subplate	Weak		1B

Structure	Level	Pattern	Image	Note
TS27: cortical layer II	Present		1D
TS27: cortical layer III	Present		1D
TS27: cortical layer IV	Present		1D
TS27: cortical layer V	Present		1D
TS27: cortical layer VI	Present		1D

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		1F	Expression progressively decreases in the subventricular/ependymal zone and in the subplate zone.
TS28: cortical layer III	Present		1F	Expression progressively decreases in the subventricular/ependymal zone and in the subplate zone.
TS28: cortical layer IV	Present		1F	Expression progressively decreases in the subventricular/ependymal zone and in the subplate zone.
TS28: cortical layer V	Present		1F	Expression progressively decreases in the subventricular/ependymal zone and in the subplate zone.
TS28: cortical layer VI	Present		1F	Expression progressively decreases in the subventricular/ependymal zone and in the subplate zone.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	1J	Expression is in the neuroepithelia of the third ventricle.

Structure	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	Strong		2D , 2B
TS28: dentate gyrus	Strong		2D , 2B
TS28: hippocampus CA1	Strong		2D , 2B

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Present	Widespread	3A
TS28: main olfactory bulb glomerular layer	Strong		3B , 3A
TS28: main olfactory bulb mitral cell layer	Strong		3B , 3A

Structure	Level	Pattern	Image	Note
TS24: choroid plexus	Very strong		3D

Structure	Level	Pattern	Image	Note
TS28: choroid plexus	Very strong		3E