Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS13: heart	Weak	Scattered	1A
TS13: somite	Strong		1A
TS13: future brain	Weak		1A
TS13: neural tube	Present		1A

Structure	Level	Pattern	Image	Note
TS13: heart	Present		1B	Expression is stronger and covers larger area than that of E8.25.

Structure	Level	Pattern	Image	Note
TS14: outflow tract	Present	Regionally restricted	1C	Expressed in the caudal wall.
TS14: primitive ventricle	Present	Regionally restricted	1C	Expressed in the cranial wall of the distal part of the ventricular loop.
TS14: future brain	Strong		1C
TS14: somite	Strong		1C

Structure	Level	Pattern	Image	Note
TS14: heart	Present	Regionally restricted	2A	Expressed in the inner curvature.

Structure	Level	Pattern	Image	Note
TS14: common atrial chamber	Present	Regionally restricted	2B	Expressed in the dorsal wall of the right atrium. Expressed in both the inner and outer myocardial wall.
TS14: outflow tract	Present	Regionally restricted	2B	Expressed in the dorsal wall of the outflow tract from the right ventricle. Expressed in the endothelial cells in the cushions within the outflow tract.

Structure	Level	Pattern	Image	Note
TS15: outflow tract	Strong	Regionally restricted	3A	Expressed in the caudal wall.
TS15: atrioventricular canal	Strong	Regionally restricted	3A	Expressed in the right.
TS15: primitive ventricle	Strong	Regionally restricted	3A	Expressed in the interventricular groove.

Structure	Level	Pattern	Image	Note
TS15: atrioventricular canal	Present	Regionally restricted	3B	Expressed in the superior wall.

Structure	Level	Pattern	Image	Note
TS15: heart atrium	Present	Regionally restricted	3C	Expressed in the right and dorsal wall of the right atrium.
TS15: primitive ventricle	Present	Regionally restricted	3C	Expressed in the dorsal wall of the right ventricle and the developing trabeculations.

Structure	Level	Pattern	Image	Note
TS15: primitive ventricle	Present	Regionally restricted	3D	Expressed in the superior portion of the interventricular groove.
TS15: outflow tract	Present	Regionally restricted	3D	Expressed in the dorsal wall.
TS15: atrioventricular canal	Present	Regionally restricted	3D	Expressed in the right wall at the anterior end close to the ventricle.

Structure	Level	Pattern	Image	Note
TS17: common atrial chamber right part	Present	Regionally restricted	4A	Expressed in the sinuatrial ring.
TS17: outflow tract	Present	Widespread	4A

Structure	Level	Pattern	Image	Note
TS21: atrioventricular canal	Present	Regionally restricted	4B	Expressed in the atrioventricular ring and the developing atrioventricular node.

Structure	Level	Pattern	Image	Note
TS17: atrioventricular canal	Present	Regionally restricted	5A	Expressed in the muscle just inferior to the atrioventricular cushion and along the junction between the atrioventricular cushion and the ventricle.

Structure	Level	Pattern	Image	Note
TS17: atrioventricular canal	Strong	Regionally restricted	5B	Strong expression in the right wall and weak staining in the left wall.
TS17: primitive ventricle	Strong	Regionally restricted	5B	Expressed in the crest of the muscular ventricular septum.

Structure	Level	Pattern	Image	Note
TS17: atrioventricular canal	Very strong	Regionally restricted	5C	Expressed in the junction between the atrioventricular canal and the right ventricle.
TS17: primitive ventricle	Very strong	Regionally restricted	5C	Expressed in the right side of the ventricular septum. The right ventricular trabeculations are extensively stained.

Structure	Level	Pattern	Image	Note
TS17: atrioventricular canal	Strong	Regionally restricted	5D	Expressed in the junction between the atrioventricular canal and the right ventricle.
TS17: primitive ventricle	Strong	Regionally restricted	5D	Expressed in the right side of the ventricular septum. The right ventricular trabeculations are extensively stained.
TS17: endocardial cushion	Present	Regionally restricted	5D	Expressed in the musculature along the superior atrioventricular cushion.

Structure	Level	Pattern	Image	Note
TS17: outflow tract	Present	Regionally restricted	5E	Expressed throughout the caudal wall of the outflow tract adjacent to the atrioventricular canal.
TS17: primitive ventricle	Present	Regionally restricted	5E	Expression bends around the ventricular groove.

Structure	Level	Pattern	Image	Note
TS17: outflow tract	Present		5F	Expressed in the walls.

Structure	Level	Pattern	Image	Note
TS19: outflow tract	Present		6A	Expressed in the wall forming an almost complete ring.
TS19: atrioventricular canal	Present	Regionally restricted	6A	Expressed in the walls producing the atrioventricular ring. Expressed in the developing atrioventricular node. Expressed in the trabeculations from both the superior and inferior myocardial walls along the canal.
TS19: interventricular septum	Present	Regionally restricted	6A	Expressed in the walls producing the interventricular ring.

Structure	Level	Pattern	Image	Note
TS19: heart right ventricle	Present	Non-Uniform	6C , 6B	Expressed in right ventricular trabeculations.
TS19: right atrium	Weak	Regionally restricted	6B	Thin staining forms a partial sinuatrial ring.

Structure	Level	Pattern	Image	Note
TS20: outflow tract	Present		6D	Expressed in the wall forming a complete ring.
TS20: heart right ventricle	Present		6D	Expressed in the right ventricular trabeculations, right atrioventricular junction, and right ventricle.
TS20: atrioventricular endocardial cushion	Present		6D	Expressed in the right atrioventricular junction.

Structure	Level	Pattern	Image	Note
TS20: outflow tract	Present		6E	Expressed in the wall forming a complete ring.
TS20: atrioventricular canal	Present		6E	Expressed in the wall forming a complete ring. Expressed in the developing atrioventricular node.

Structure	Level	Pattern	Image	Note
TS21: heart ventricle	Present	Regionally restricted	7A	Expressed in the superior portion of the ventricular wall.

Structure	Level	Pattern	Image	Note
TS21: interventricular septum	Present	Regionally restricted	7B	Expressed along the crest of the ventricular septum, forming the primordial branching bundles.

Structure	Level	Pattern	Image	Note
TS21: outflow tract	Strong	Regionally restricted	7C	Strong retroaortic staining.
TS21: tricuspid valve	Strong	Regionally restricted	7C	Expressed in the musculature surrounding the developing tricuspid valve.

Structure	Level	Pattern	Image	Note
TS21: atrioventricular canal	Present	Regionally restricted	7D	Expressed in the developing atrioventricular conduction axis and in the right and left atrioventricular rings.

Structure	Level	Pattern	Image	Note
TS27: pulmonary trunk	Present	Regionally restricted	8A	Expressed in the anterior portion of the ring around the pulmonary outflow.

Structure	Level	Pattern	Image	Note
TS27: aorta	Present	Regionally restricted	8B	Expressed in the aorta as a ring.
TS27: mitral valve	Present	Regionally restricted	8B	Expressed in the musculature.
TS27: atrioventricular bundle	Present	Regionally restricted	8B	Expressed along the left branching bundle.
TS27: heart	Present	Regionally restricted	8B	Expressed as an atrioventricular ring.

Structure	Level	Pattern	Image	Note
TS27: atrioventricular bundle	Present		8C	His bundle.
TS27: outflow tract	Present	Regionally restricted	8C	Expressed in the retroaortic tissue.

Structure	Level	Pattern	Image	Note
TS27: tricuspid valve	Present	Regionally restricted	8D	Expressed along the musculature.
TS27: impulse conducting system	Present	Regionally restricted	8D	Expressed in the conduction axis, which has penetrated into the atrial septum.
TS27: heart	Present	Regionally restricted	8D	Expressed as an atrioventricular ring.

Structure	Level	Pattern	Image	Note
TS27: impulse conducting system	Present	Regionally restricted	8E	Expressed in the atrioventricular conduction axis.
TS27: heart	Present	Regionally restricted	8E	Expressed as an atrioventricular ring.

Structure	Level	Pattern	Image	Note
TS27: vena cava	Strong	Regionally restricted	8F	Expressed in the musculature at the boundary between the systemic venous tributaries and the right atrium.
TS27: atrium endocardial lining	Present	Scattered	8F	Scattered staining along the endocardial side of the right atrial wall.
TS27: right atrium	Present	Scattered	8F	Scattered staining along the endocardial side of the right atrial wall.

Structure	Level	Pattern	Image	Note
TS19: heart left ventricle	Present

Structure	Level	Pattern	Image	Note
TS21: ascending aorta	Present	Regionally restricted		Expressed in the endothelial linings.
TS21: pulmonary artery	Present	Regionally restricted		Expressed in the endothelial linings.