Gene Expression Data
Assay Details
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Assay
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Reference:
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J:183957
Wu F, et al., Onecut 1 and Onecut 2 are potential regulators of mouse retinal development. J Comp Neurol. 2012 Apr 1;520(5):952-69
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5509358
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Gene symbol:
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Onecut2
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Gene name:
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one cut domain, family member 2
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Results
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Specimen
5H,I:
postnatal day 16
(more  )
Note: Double labeled: magenta - Onecut2; green - Calb1 (assay MGI:5509356).
Specimen
5H,I:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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postnatal day 16
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: magenta - Onecut2; green - Calb1 (assay MGI:5509356).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina inner nuclear layer |
Present |
Regionally restricted |
5H
,
5I
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Expression was detected in horizontal cells. There was overlap with the expression of the horizontal cell marker Calbindin.
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Specimen
5K,L:
postnatal day 16
(more )
Note: Double labeled: magenta - Onecut2; green - Lhx1 (assay MGI:5509361).
Specimen
5K,L:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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postnatal day 16
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: magenta - Onecut2; green - Lhx1 (assay MGI:5509361).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina inner nuclear layer |
Present |
Regionally restricted |
5K
,
5L
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Expression was detected in horizontal cells. There was overlap with the expression of the horizontal cell marker Lim1.
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Specimen
5N,N',N''':
embryonic day 14.5
(more )
Note: Double labeled: magenta - Onecut2; green - Lhx1 (assay MGI:5509361).
Specimen
5N,N',N''':
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: magenta - Onecut2; green - Lhx1 (assay MGI:5509361).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Present |
Regionally restricted |
5N'''
,
5N
,
5N'
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Expression was detected in Lim1-positive horizontal cell precursors.
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Specimen
8G:
embryonic day 14.5; Atoh7tm1Gan/Atoh7 +
(more )
Specimen
8G:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Atoh7tm1Gan/Atoh7+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Strong |
Regionally restricted |
8G
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Expression was detected in a narrow band in the inner most regions of the retina.
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Specimen
8H:
embryonic day 14.5; Pou4f2tm2Whk/Pou4f2 +
(more )
Specimen
8H:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Pou4f2tm2Whk/Pou4f2+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
8I:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCr
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Mutant Allele(s):
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Isl1tm1.2Whk/Isl1+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
8J:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Atoh7tm1Gan/Atoh7tm1Gan
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Strong |
Regionally restricted |
8J
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Expression was detected in a narrow band in the inner most regions of the retina as in wild type. There was no change in the level or pattern of expression in the mutant.
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Specimen
8K:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Pou4f2tm2Whk/Pou4f2tm2Whk
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Present |
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8K
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There was no change in the expression pattern or level in the mutant compared to the control.
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Specimen
8L:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCr
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Mutant Allele(s):
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Isl1tm1.2Whk/Isl1tm1.2Whk
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Present |
Regionally restricted |
8L
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The expression pattern did not change in the mutnat, but the level was markedly higher in both the neuroblast layer and the ganglion cell layer.
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Analysis Tools