Gene Expression Data
Assay Details
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Assay
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Reference:
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J:73289
Estivill-Torrus G, et al., The transcription factor Pax6 is required for development of the diencephalic dorsal midline secretory radial glia that form the subcommissural organ. Mech Dev. 2001 Dec;109(2):215-24
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5508802
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Gene symbol:
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Cdh4
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Gene name:
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cadherin 4
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Antibody:
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R-cadherin (H-66): sc-7941
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
4D:
embryonic day 18.5
(more  )
Specimen
4D:
(close  )
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Genetic Background:
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involves: Swiss
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: midbrain |
Strong |
Regionally restricted |
4D
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Expression was detected in the subcommissural organ.
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Specimen
4E:
embryonic day 18.5; Pax6Sey/Pax6 +
(more )
Specimen
4E:
(close )
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Genetic Background:
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involves: Swiss
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Mutant Allele(s):
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Pax6Sey/Pax6+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: midbrain |
Present |
Regionally restricted |
4E
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Expression was detected in the subcommissural organ perikarya, but there was a drastic reduction of the label in the basal processes of subcommissural organ cells.
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Specimen
4F:
(close )
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Genetic Background:
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involves: Swiss
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: midbrain |
Weak |
Regionally restricted |
4F
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Only weak immunoreactivity was detected in the ependyma.
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Analysis Tools