Probe:	Neuronatin cDNA1
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS16: future hindbrain ventricular layer	Strong	Regionally restricted	5A	Expression is in the ventral ventricular zone.
TS16: branchial arch mesenchyme derived from neural crest	Strong		5A
TS16: diencephalon lamina terminalis	Present		5A
TS16: head mesenchyme derived from neural crest	Present		5A
TS16: neural tube lateral wall	Present	Regionally restricted	5A	Expression is in the ventral part of the undifferentiated ventricular zone.
TS16: surface ectoderm	Present	Regionally restricted	5A	Expression is in the ventral midline surface ectoderm.
TS16: future midbrain	Absent		5A

Structure	Level	Pattern	Image	Note
TS16: branchial arch mesenchyme derived from neural crest	Strong		5B
TS16: Rathke's pouch	Present		5B
TS16: diencephalon lamina terminalis	Present		5B
TS16: head mesenchyme derived from neural crest	Present		5B
TS16: surface ectoderm	Present	Regionally restricted	5B	Expression is in the ventral midline surface ectoderm.
TS16: mesenchyme derived from splanchnopleure	Present	Regionally restricted	5B	Expression is in splanchnic mesoderm of the hindgut.
TS16: future midbrain	Absent		5B

Structure	Level	Pattern	Image	Note
TS16: Rathke's pouch	Present	Regionally restricted	5C	Expression is in the intermediate zone.
TS16: future hindbrain	Present	Regionally restricted	5C	Expression is in the intermediate zone.
TS16: diencephalon lamina terminalis	Present		5C
TS16: facio-acoustic ganglion complex	Present		5C
TS16: trigeminal ganglion	Present		5C
TS16: future midbrain	Absent		5C

Structure	Level	Pattern	Image	Note
TS16: branchial arch mesenchyme derived from neural crest	Strong		5D
TS16: diencephalon lamina terminalis	Present		5D
TS16: facio-acoustic ganglion complex	Present		5D
TS16: trigeminal ganglion	Present		5D
TS16: future hindbrain	Present	Regionally restricted	5D	Expression is in the intermediate zone.
TS16: future midbrain	Absent		5D

Structure	Level	Pattern	Image	Note
TS16: paraxial mesenchyme	Present		5E
TS16: somite	Present		5E

Structure	Level	Pattern	Image	Note
TS17: rhombomere ventricular layer	Strong	Regionally restricted	6A/B	Expression is in the ventral ventricular zone.
TS17: hindbrain lateral wall	Present	Regionally restricted	6A/B	Expression is in the intermediate zone.
TS17: diencephalon	Present	Regionally restricted	6A/B	Expression is ventral.
TS17: eye	Present	Regionally restricted	6A/B	Expression is in the optic chiasma.
TS17: telencephalon	Present	Regionally restricted	6A/B	Expression is in the intermediate zone.

Structure	Level	Pattern	Image	Note
TS17: rhombomere ventricular layer	Strong	Regionally restricted	6C/D	Expression is in the ventral ventricular zone.
TS17: hindbrain lateral wall	Present	Regionally restricted	6C/D	Expression is in the intermediate zone.
TS17: diencephalon	Present	Regionally restricted	6C/D	Expression is ventral.
TS17: eye	Present	Regionally restricted	6C/D	Expression is in the optic chiasma and the preoccular muscle mass.
TS17: telencephalon	Present	Regionally restricted	6C/D	Expression is in the intermediate zone.
TS17: Rathke's pouch	Present		6C/D

Structure	Level	Pattern	Image	Note
TS17: rhombomere ventricular layer	Strong	Regionally restricted	6E/F	Expression is in the ventral ventricular zone.
TS17: hindbrain lateral wall	Present	Regionally restricted	6E/F	Expression is in the intermediate zone.

Structure	Level	Pattern	Image	Note
TS21: medulla oblongata	Strong		7A/D
TS21: metencephalon	Strong		7A/D
TS21: choroid plexus	Absent		7A/D

Structure	Level	Pattern	Image	Note
TS21: medulla oblongata	Strong		7B/E
TS21: metencephalon	Strong		7B/E
TS21: facial ganglion	Present		7B/E
TS21: pinna mesenchyme	Present		7B/E
TS21: remnant of Rathke's pouch	Present		7B/E
TS21: trigeminal ganglion	Present		7B/E
TS21: vestibulo-cochlear ganglion	Present		7B/E
TS21: retina	Present	Regionally restricted	7B/E	Expression is in the inner neural layer.
TS21: choroid plexus	Absent		7B/E

Structure	Level	Pattern	Image	Note
TS21: facial ganglion	Present		7C/F
TS21: pinna mesenchyme	Present		7C/F
TS21: trigeminal ganglion	Present		7C/F
TS21: vestibulo-cochlear ganglion	Present		7C/F
TS21: retina	Present	Regionally restricted	7C/F	Expression is in the inner neural layer.
TS21: cochlear duct mesenchyme	Present	Regionally restricted	7C/F	Expression is in the mesenchyme surrunding the neural epithelium in the cochlea.
TS21: spinal cord	Very strong		7C/F	Expression is in the intermediate zone.
TS21: choroid plexus	Absent		7C/F

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Very strong		7G/I	Expression is in the intermediate zone.
TS23: medulla oblongata	Strong		7G/I
TS23: metencephalon	Strong		7G/I
TS23: dorsal root ganglion	Present		7G/I
TS23: peripheral nerve trunk	Present		7G/I
TS23: choroid plexus	Absent		7G/I

Structure	Level	Pattern	Image	Note
TS23: medulla oblongata	Strong		7H/J
TS23: metencephalon	Strong		7H/J
TS23: dorsal root ganglion	Present		7H/J
TS23: choroid plexus	Absent		7H/J

Structure	Level	Pattern	Image	Note
TS23: cochlea epithelium	Present		7K/L
TS23: dorsal root ganglion	Present		7K/L
TS23: facial ganglion	Present		7K/L
TS23: limb mesenchyme	Present		7K/L
TS23: mandible	Present		7K/L
TS23: maxilla	Present		7K/L
TS23: tongue mesenchyme	Present		7K/L
TS23: trigeminal ganglion	Present		7K/L
TS23: vestibulo-cochlear ganglion	Present		7K/L
TS23: cochlear duct mesenchyme	Present	Regionally restricted	7K/L	Expression is in the mesenchyme surrunding the neural epithelium in the cochlea.

Structure	Level	Pattern	Image	Note
TS16: foregut	Absent

Structure	Level	Pattern	Image	Note
TS17: otocyst	Present	Regionally restricted		Expression is in the lateral epithelium of the otic vesicle.
TS17: acoustic ganglion	Present
TS17: facial ganglion	Present
TS17: glossopharyngeal ganglion	Present
TS17: trigeminal ganglion	Present
TS17: vagus ganglion	Present

Structure	Level	Pattern	Image	Note
TS21: peripheral nerve trunk	Present

Structure	Level	Pattern	Image	Note
TS23: remnant of Rathke's pouch	Present

Structure	Level	Pattern	Image	Note
TS25: medulla oblongata	Strong
TS25: metencephalon	Strong
TS25: remnant of Rathke's pouch	Present
TS25: retina inner nuclear layer	Present